• Corpus ID: 10883995

Cytokeratin characterization of human prostatic carcinoma and its derived cell lines.

@article{Nagle1987CytokeratinCO,
  title={Cytokeratin characterization of human prostatic carcinoma and its derived cell lines.},
  author={Raymond B. Nagle and Frederick R. Ahmann and Kathy M. McDaniel and M L Paquin and Virginia A. Clark and Abbie Cheryl Celniker},
  journal={Cancer research},
  year={1987},
  volume={47 1},
  pages={
          281-6
        },
  url={https://api.semanticscholar.org/CorpusID:10883995}
}
It was concluded that during both hyperplasia and neoplastic transformation of the prostate, the luminal cell phenotype is primarily involved and that the basal cell phenotype does not appear to contribute to either intraluminal proliferation or invasive cell populations.

Figures from this paper

Differential expression of specific cytokeratin polypeptides in the basal and luminal epithelia of the human prostate

Results indicate that antibodies to specific cytokeratin polypeptides can be used not only to differentiate between prostatic basal and luminal cells but also to study the biological processes of prostatic organogenesis and carcinogenesis.

Colocalization of basal and luminal cell-type cytokeratins in human prostate cancer.

Keratin antibodies were used to obtain a better insight into the different cell types involved in the etiology and progression of prostatic carcinoma to postulate that on the basis of the keratin expression profile this subpopulation is most likely the target for neoplastic transformation.

Expression of basal cell keratins in human prostate cancer metastases and cell lines

Accumulation of intermediate cells in regressed and hormone‐escaped prostate cancer indicates that for their survival, these cells are androgen‐independent.

Basal cells of H-Dunning tumor are myoepithelial cells

A comparative immunohistochemical and ultrastructural study on the Dunning tumor and rat prostate lobes found that basal cells in human prostatic epithelium are not myoepithelial cells, making this finding difficult to reconcile with the contention of a prostatic origin of the H-Dunning tumor.

Keratin immunoreactivity as an aid to the diagnosis of persistent adenocarcinoma in irradiated human prostates

The interpretation of postirradiation prostatic biopsy specimens may be aided by immunohistochemistry with a specific monoclonal anticytokeratin antibody (KA1) that reacts with the basal cells of normal or hyperplastic glands, but is nonreactive with the lumenal cells or with prostatic carcinoma cells.

Squamous metaplasia of the prostate. An immunohistochemical study.

Findings suggest that although prostatic glandular epithelial cells retain their ability to express some prostate-associated antigens, this ability is greatly reduced, lost, or not developed in cells that undergo metaplasia into squamous cells or that develop intoSquamous cell carcinoma.
...

Establishment and characterization of a human prostatic carcinoma cell line (PC-3).

The establishment, characterization, and tumorigenicity of a new epithelial cell line (PC-3) from a human prostatic adenocarcinoma metastatic to bone is reported, which should be useful in investigating the biochemical changes in advanced prostatic cancer cells and in assessing their response to chemotherapeutic agents.

Keratin immunoreactivity in the benign and neoplastic human prostate.

The findings indicate that keratin immunoreactivity differs among the epithelial cell populations of the human prostate, probably reflecting expression of different keratin proteins.

Characterization of breast carcinomas by two monoclonal antibodies distinguishing myoepithelial from luminal epithelial cells.

Two monoclonal antibodies, KA 1 and KA 4, raised against human epidermis, were biochemically and immunologically characterized and were shown to react with specific cytokeratin polypeptides and distinguish between myoepithelial and luminal epithelial cells on frozen sections of human mammary gland.

Isolation of a human prostate carcinoma cell line (DU 145)

Electron microscopy of the original tumor tissue and of the tissue culture cell line show a remarkable similarity in cell organelle structure.

Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology.

RGE 53 can distinguish adenocarcinomas and mesotheliomas, which stain positively in the indirect immunofluorescence technique from squamous cell carcinomas and nonepithelial tumors, which are negative for this antibody.

Immunohistochemical localization of keratin in normal human tissues.

The ability to identify keratin proteins within fixed, embedded tissue (including those known to lack tonofilament bundles) may prove useful in the study of tissue histogenesis and carcinogenesis, and in the pathologic assessment of poorly differentiated malignant neoplasms and tumors of controversial cellular origin.

Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods.

The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied.

Identification and characterization of epithelial cells in mammalian tissues by immunofluorescence microscopy using antibodies to prekeratin.

The widespread, if not general occurrence of cytokeratin filaments in epithelial cells is emphasized, and it is proposed to use this specific structure as a criterion for true epithelial character or origin.

Cytokeratin polypeptide patterns of different epithelia of the human male urogenital tract: immunofluorescence and gel electrophoretic studies.

The results shows that the wide spectrum of histological differentiation of the diverse epithelia present in the male urogenital tract is accompanied by pronounced changes in the expression of cytokeratin polypeptides and suggest that tumors from different regions of the urogensital tract may be distinguished by their cytokersatin complements.