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Joanna Gerszon, Eligiusz Serafin, Adam Buczkowski, Sylwia Michlewska, Jakub Antoni Bielnicki, Aleksandra Rodacka
Functional consequences of piceatannol binding to glyceraldehyde-3-phosphate dehydrogenase
www.plosone.org
www.plosone.org
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-35.0586 -1.2982 Td
(seladin-1)Tj
3.855 0 Td
(mRNA)Tj
3.0784 0 Td
(on)Tj
1.2756 0 Td
(constant)Tj
3.6397 0 Td
(level)Tj
2.0126 0 Td
([)Tj
0.83 0.64 0.02 0 k
(17)Tj
0 g
(].)Tj
-12.6651 -1.2983 Td
(Therefore,)Tj
4.3936 0 Td
(the)Tj
1.4684 0 Td
(main)Tj
2.2904 0 Td
(aim)Tj
1.7404 0 Td
(of)Tj
1.0148 0 Td
(this)Tj
1.6781 0 Td
(research)Tj
3.5546 0 Td
(was)Tj
1.6951 0 Td
(to)Tj
1.0262 0 Td
(establish)Tj
3.6453 0 Td
(if)Tj
0.7767 0 Td
(piceatannol)Tj
4.8699 0 Td
(binds)Tj
2.4321 0 Td
(to)Tj
1.0205 0 Td
(GAPDH)Tj
-32.8023 -1.2983 Td
(and)Tj
1.7291 0 Td
(whether)Tj
3.4752 0 Td
(this)Tj
1.6725 0 Td
(interaction)Tj
4.6091 0 Td
(affects)Tj
2.7382 0 Td
(protein's)Tj
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(functional)Tj
4.3086 0 Td
(properties)Tj
4.2746 0 Td
(and)Tj
1.7292 0 Td
(inhibits)Tj
3.2654 0 Td
(GAPDH)Tj
-31.5323 -1.3039 Td
(aggregation.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 616.1385 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Materials)Tj
4.5071 0 Td
(and)Tj
1.989 0 Td
(methods)Tj
/F0 1 Tf
-6.4961 -1.5023 Td
(Materials)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 581.1023 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Glyceraldehyde-3-phosphate)Tj
11.707 0 Td
(dehydrogenase)Tj
6.2191 0 Td
(\(GAPDH\))Tj
4.3597 0 Td
(from)Tj
2.2053 0 Td
(rabbit)Tj
2.6079 0 Td
(muscle,)Tj
3.2428 0 Td
(trans-3,5,3',4'-tet-)Tj
-30.3418 -1.2982 Td
(rahydroxistilbene)Tj
7.2226 0 Td
(\(piceatannol,)Tj
5.4311 0 Td
(PIC\),)Tj
2.3471 0 Td
(trans-3,5,4)Tj
ET
Q
q
1 j
1 J
0 w
6.9998 0 0 6.9998 392.4283 571.748 cm
BT
/F13 1 Tf
1 TL
0 0 Td
(0)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 394.8094 568.1196 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(-trihydroxystilbene)Tj
7.8916 0 Td
(\(resveratrol,)Tj
5.0513 0 Td
(RSV\))Tj
2.3527 0 Td
(and)Tj
1.7291 0 Td
(all)Tj
-36.5042 -1.2982 Td
(other)Tj
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(chemicals)Tj
4.1442 0 Td
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2.126 0 Td
(purchased)Tj
4.3313 0 Td
(from)Tj
2.2053 0 Td
(Sigma-Aldrich)Tj
6.083 0 Td
(chemical)Tj
3.7814 0 Td
(Co)Tj
1.389 0 Td
(\(St.)Tj
1.5647 0 Td
(Louis,)Tj
2.6419 0 Td
(MO,)Tj
2.0863 0 Td
(USA\),)Tj
-32.7002 -1.3039 Td
(unless)Tj
2.6872 0 Td
(otherwise)Tj
4.0819 0 Td
(stated.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 515.8488 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
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5.3244 0 Td
(of)Tj
1.0441 0 Td
(protein)Tj
3.2363 0 Td
(and)Tj
1.8661 0 Td
(polyphenol)Tj
4.9843 0 Td
(solutions)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 498.8409 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Protein)Tj
3.1917 0 Td
(solution)Tj
3.4866 0 Td
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1.6951 0 Td
(prepared)Tj
3.8098 0 Td
(by)Tj
1.1792 0 Td
(dissolving)Tj
4.2236 0 Td
(GAPDH)Tj
3.685 0 Td
(in)Tj
1.0375 0 Td
(0.02)Tj
1.8708 0 Td
(M)Tj
1.1112 0 Td
(sodium)Tj
3.2315 0 Td
(phosphate)Tj
4.3313 0 Td
(buffer)Tj
2.6249 0 Td
(at)Tj
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(pH)Tj
1.5023 0 Td
(7.4.)Tj
1.6214 0 Td
(Protein)Tj
3.1975 0 Td
(concentration)Tj
5.8166 0 Td
(was)Tj
1.7008 0 Td
(determined)Tj
4.8529 0 Td
(spectrophotometrically)Tj
9.462 0 Td
(at)Tj
0.9524 0 Td
(280)Tj
1.6441 0 Td
(nm,)Tj
1.8142 0 Td
(using)Tj
2.3924 0 Td
(the)Tj
-34.9566 -1.3039 Td
(extinction)Tj
4.2632 0 Td
(coefficient)Tj
4.3824 0 Td
(E)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 292.1385 471.1748 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(1%)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 303.5905 472.8188 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(=)Tj
0.7993 0 Td
(10)Tj
1.1679 0 Td
(\(GAPDH,)Tj
4.2519 0 Td
(MW)Tj
2.075 0 Td
(=)Tj
0.7993 0 Td
(143,000\).)Tj
3.8665 0 Td
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1.7801 0 Td
(stock)Tj
2.3074 0 Td
(solutions)Tj
3.8438 0 Td
(of)Tj
1.0148 0 Td
(piceatannol)Tj
-32.2637 -1.2982 Td
(and)Tj
1.7291 0 Td
(resveratrol)Tj
4.4844 0 Td
(were)Tj
2.1203 0 Td
(freshly)Tj
2.8912 0 Td
(prepared)Tj
3.8098 0 Td
(by)Tj
1.1792 0 Td
(dissolving)Tj
4.2236 0 Td
(the)Tj
1.474 0 Td
(commercial)Tj
4.9549 0 Td
(powders)Tj
3.6113 0 Td
(in)Tj
1.0318 0 Td
(phosphate)Tj
-31.5096 -1.2983 Td
(buffer,)Tj
2.8517 0 Td
(in)Tj
1.0318 0 Td
(dark.)Tj
2.279 0 Td
(The)Tj
1.7801 0 Td
(molar)Tj
2.5965 0 Td
(extinction)Tj
4.2689 0 Td
(coefficient)Tj
4.3767 0 Td
(for)Tj
1.3889 0 Td
(piceatannol)Tj
4.8643 0 Td
(was)Tj
1.7007 0 Td
(33100)Tj
2.5966 0 Td
(M)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 506.3244 451.2755 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 512.5039 446.8535 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(cm)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 524.9196 451.2755 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 533.3102 446.8535 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(at)Tj
0.9524 0 Td
(the)Tj
-34.2819 -1.3039 Td
(maximal)Tj
3.702 0 Td
(wavelength)Tj
4.7282 0 Td
(of)Tj
1.0148 0 Td
(326)Tj
1.6497 0 Td
(nm)Tj
1.5874 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(],)Tj
2.075 0 Td
(and)Tj
1.7291 0 Td
(30335)Tj
2.6022 0 Td
(M)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 399.7984 438.2929 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 405.9779 433.8141 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(cm)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 418.3937 438.2929 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 426.7842 433.8141 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(for)Tj
1.3832 0 Td
(resveratrol)Tj
4.4844 0 Td
(at)Tj
0.9525 0 Td
(the)Tj
1.4683 0 Td
(wavelength)Tj
4.7282 0 Td
(of)Tj
-35.6936 -1.2982 Td
(304)Tj
1.6441 0 Td
(nm)Tj
1.5874 0 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 394.5826 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Assay)Tj
2.9197 0 Td
(of)Tj
1.0441 0 Td
(GAPDH)Tj
3.7512 0 Td
(activity)Tj
3.2457 0 Td
(in)Tj
0.9827 0 Td
(the)Tj
1.5921 0 Td
(presence)Tj
4.2851 0 Td
(of)Tj
1.0441 0 Td
(piceatannol)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 377.5748 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(activity)Tj
3.1181 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(GAPDH)Tj
3.685 0 Td
(\(2)Tj
/F14 1 Tf
1.0375 0 Td
[()]TJ
/F7 1 Tf
(M\))Tj
1.9559 0 Td
(and)Tj
1.7291 0 Td
(LDH)Tj
2.2621 0 Td
(\(2)Tj
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1.0374 0 Td
[()]TJ
/F7 1 Tf
(M\))Tj
1.9616 0 Td
(was)Tj
1.6951 0 Td
(determined)Tj
4.8529 0 Td
(spectrophotometrically)Tj
-27.5979 -1.3039 Td
(by)Tj
1.1792 0 Td
(measuring)Tj
4.439 0 Td
(the)Tj
1.4683 0 Td
(rate)Tj
1.7462 0 Td
(of)Tj
1.0147 0 Td
(reduction)Tj
4.1159 0 Td
(of)Tj
1.0148 0 Td
(NAD)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 371.3952 369.0141 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 377.9149 364.5354 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(to)Tj
1.0261 0 Td
(NADH)Tj
3.1408 0 Td
(\(in)Tj
1.3776 0 Td
(case)Tj
1.8652 0 Td
(of)Tj
1.0148 0 Td
(GAPDH\))Tj
4.0195 0 Td
(or)Tj
1.0998 0 Td
(oxidation)Tj
4.0422 0 Td
(of)Tj
-35.3761 -1.2982 Td
(NADH)Tj
3.1408 0 Td
(to)Tj
1.0261 0 Td
(NAD)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 263.2818 356.0314 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 269.8015 351.5527 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\(for)Tj
1.7234 0 Td
(LDH\),)Tj
2.829 0 Td
(what)Tj
2.1656 0 Td
(has)Tj
1.5421 0 Td
(been)Tj
2.1203 0 Td
(described)Tj
4.0535 0 Td
(in)Tj
1.0374 0 Td
(details)Tj
2.778 0 Td
(previously)Tj
4.3483 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(].)Tj
2.0806 0 Td
(The)Tj
1.7802 0 Td
(relative)Tj
-33.4372 -1.2982 Td
(activity)Tj
3.1181 0 Td
(of)Tj
1.0148 0 Td
(enzymes)Tj
3.6622 0 Td
(incubating)Tj
4.5071 0 Td
(with)Tj
1.9956 0 Td
(various)Tj
3.1464 0 Td
(concentrations)Tj
6.1795 0 Td
(of)Tj
1.0148 0 Td
(piceatannol)Tj
4.8642 0 Td
(\(0;)Tj
1.2642 0 Td
(3.2;)Tj
1.6215 0 Td
(16;)Tj
1.3946 0 Td
(32;)Tj
-33.783 -1.2983 Td
(64)Tj
/F14 1 Tf
1.1679 0 Td
[()]TJ
/F7 1 Tf
(M\))Tj
1.9615 0 Td
(was)Tj
1.7008 0 Td
(expressed)Tj
4.0931 0 Td
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(a)Tj
0.652 0 Td
(percentage)Tj
4.5524 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(activity)Tj
3.1181 0 Td
(of)Tj
1.0148 0 Td
(control)Tj
3.1181 0 Td
(\(enzyme)Tj
3.6453 0 Td
(without)Tj
3.3279 0 Td
(piceatannol\))Tj
-31.8498 -1.3039 Td
(and)Tj
1.7291 0 Td
(it)Tj
0.788 0 Td
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1.6952 0 Td
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4.8527 0 Td
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2.9254 0 Td
(120)Tj
1.6497 0 Td
(min)Tj
1.8539 0 Td
(of)Tj
1.0148 0 Td
(incubation.)Tj
4.7735 0 Td
(Since)Tj
2.3584 0 Td
(the)Tj
1.4683 0 Td
(effect)Tj
2.3698 0 Td
(of)Tj
1.0148 0 Td
(piceatannol)Tj
4.8642 0 Td
(on)Tj
1.2756 0 Td
(LDH)Tj
-34.6334 -1.2983 Td
(activity)Tj
3.1181 0 Td
(was)Tj
1.6951 0 Td
(not)Tj
1.576 0 Td
(observed,)Tj
4.0194 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4684 0 Td
(figure)Tj
2.5625 0 Td
(there)Tj
2.2677 0 Td
(are)Tj
1.4456 0 Td
(presented)Tj
4.1159 0 Td
(data)Tj
1.9162 0 Td
(only)Tj
1.9729 0 Td
(for)Tj
1.389 0 Td
(LDH)Tj
2.262 0 Td
(treated)Tj
2.9934 0 Td
(with)Tj
2.0012 0 Td
(the)Tj
-35.8409 -1.2982 Td
(highest)Tj
3.0897 0 Td
(concentration)Tj
5.8166 0 Td
(of)Tj
1.0148 0 Td
(compound)Tj
4.5978 0 Td
(\(64)Tj
/F14 1 Tf
1.5137 0 Td
[()]TJ
/F7 1 Tf
(M\).)Tj
2.1826 0 Td
(All)Tj
1.4003 0 Td
(measurements)Tj
6.0321 0 Td
(were)Tj
2.1203 0 Td
(carried)Tj
3.0387 0 Td
(out)Tj
1.5534 0 Td
(at)Tj
0.9524 0 Td
(24)Tj
/F14 1 Tf
1.1736 0 Td
[()]TJ
/F7 1 Tf
0.788 0 Td
(1C)Tj
-35.274 -1.3039 Td
(using)Tj
2.3868 0 Td
(a)Tj
0.6576 0 Td
(CARY-1)Tj
3.6453 0 Td
(apparatus)Tj
4.1158 0 Td
(\(Varian,)Tj
3.5319 0 Td
(Melbourne,)Tj
4.8926 0 Td
(Australia\).)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 247.2944 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Detection)Tj
4.3937 0 Td
(of)Tj
1.0441 0 Td
(GAPDH)Tj
3.7559 0 Td
(thiols)Tj
2.5182 0 Td
(during)Tj
2.9575 0 Td
(incubation)Tj
4.715 0 Td
(with)Tj
1.9748 0 Td
(piceatannol)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 230.2866 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Detection)Tj
4.1442 0 Td
(of)Tj
1.0148 0 Td
(relative)Tj
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(content)Tj
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(of)Tj
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(protein)Tj
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(SH)Tj
1.4627 0 Td
(groups)Tj
2.9707 0 Td
(was)Tj
1.7008 0 Td
(based)Tj
2.4718 0 Td
(on)Tj
1.2755 0 Td
(the)Tj
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(formation)Tj
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(color)Tj
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(resulting)Tj
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(reaction)Tj
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(of)Tj
1.0148 0 Td
(DTNB)Tj
2.8856 0 Td
(\(Ellman's)Tj
3.9968 0 Td
(reagent\).)Tj
3.7531 0 Td
(Absorbance)Tj
4.9833 0 Td
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0.9524 0 Td
(412)Tj
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(nm)Tj
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(was)Tj
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(measured)Tj
-33.4202 -1.3039 Td
(spectrophotometrically)Tj
9.4619 0 Td
(in)Tj
1.0375 0 Td
(denaturation)Tj
5.4028 0 Td
(condition)Tj
4.1159 0 Td
(\(4)Tj
1.0375 0 Td
(M)Tj
1.1111 0 Td
(guanidine-HCl\).)Tj
6.8258 0 Td
(Relative)Tj
3.3846 0 Td
(changes)Tj
3.4072 0 Td
(in)Tj
-35.7843 -1.2983 Td
(the)Tj
1.4683 0 Td
(content)Tj
3.2655 0 Td
(of)Tj
1.0148 0 Td
(free)Tj
1.7291 0 Td
(SH)Tj
1.4627 0 Td
(groups)Tj
2.9707 0 Td
(in)Tj
1.0318 0 Td
(GAPDH)Tj
3.685 0 Td
(exposed)Tj
3.4469 0 Td
(on)Tj
1.2756 0 Td
(piceatannol)Tj
4.8642 0 Td
(was)Tj
1.7008 0 Td
(presented)Tj
4.1158 0 Td
(as)Tj
1.0148 0 Td
(relative)Tj
-33.046 -1.2983 Td
(intensity)Tj
3.685 0 Td
(of)Tj
1.0148 0 Td
(absorbance.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 151.9937 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Isothermal)Tj
4.8378 0 Td
(titration)Tj
3.4583 0 Td
(calorimetry)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 134.9858 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(thermal)Tj
3.3392 0 Td
(effects)Tj
2.7269 0 Td
(of)Tj
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