%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Sonia González Medina, Carolyne Hyde, Imogen Lovera, Richard J. Piercy
Detection of equine atypical myopathy-associated hypoglycin A in plant material: Optimisation and validation of a novel LC-MS based method without derivatisation
www.plosone.org
www.plosone.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 15 0 R/Annots 16 0 R/Contents 17 0 R/TrimBox[0 0 612 792]>>
endobj
16 0 obj
[18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R 33 0 R]
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref008)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref018)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref019)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref020)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref021)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref022)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref023)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref024)>>
endobj
26 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref025)>>
endobj
27 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref002)>>
endobj
28 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref009)>>
endobj
29 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref026)>>
endobj
30 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref027)>>
endobj
31 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref029)>>
endobj
32 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref027)>>
endobj
33 0 obj
<>/Border[0 0 0]/A 34 0 R>>
endobj
34 0 obj
<>
endobj
17 0 obj
[35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R 43 0 R 44 0 R 45 0 R 46 0 R 47 0 R 48 0 R 49 0 R 50 0 R 51 0 R 52 0 R]
endobj
35 0 obj
<>stream
q
0.83 0.64 0.02 0 k
203.4142 693.2976 m
208.1764 693.2976 l
h
f*
212.5984 693.2976 m
222.1228 693.2976 l
h
f*
226.6016 693.2976 m
236.0693 693.2976 l
h
f*
422.1921 693.2976 m
431.6598 693.2976 l
h
f*
221.7827 628.3276 m
231.3071 628.3276 l
h
f*
235.7858 628.3276 m
245.3102 628.3276 l
h
f*
546.0661 615.3449 m
555.5906 615.3449 l
h
f*
535.1811 589.3228 m
544.6488 589.3228 l
h
f*
549.1276 589.3228 m
558.652 589.3228 l
h
f*
342.7087 563.3008 m
347.4709 563.3008 l
h
f*
548.1071 524.2961 m
552.8693 524.2961 l
h
f*
557.348 524.2961 m
566.8724 524.2961 l
h
f*
481.663 485.348 m
491.1874 485.348 l
h
f*
496.3465 485.348 m
505.8709 485.348 l
h
f*
487.9559 472.3087 m
497.4803 472.3087 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 211.9748 707.4141 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Early)Tj
2.296 0 Td
(techniques)Tj
4.5297 0 Td
(used)Tj
2.058 0 Td
(for)Tj
1.3889 0 Td
(HGA)Tj
2.3868 0 Td
(detection)Tj
3.9401 0 Td
(in)Tj
1.0318 0 Td
(plant)Tj
2.2677 0 Td
(tissues)Tj
2.8177 0 Td
(relied)Tj
2.4774 0 Td
(on)Tj
1.2756 0 Td
(paper)Tj
2.4831 0 Td
(chromatography)Tj
-30.149 -1.3039 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2585 0 Td
(18)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.4003 0 Td
(19)Tj
0 g
(])Tj
1.508 0 Td
(and)Tj
1.7292 0 Td
(later,)Tj
2.2223 0 Td
(on)Tj
1.2756 0 Td
(ion-exchange)Tj
5.6012 0 Td
(chromatography)Tj
6.8825 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(].)Tj
2.0749 0 Td
(Both)Tj
2.1317 0 Td
(techniques)Tj
4.5297 0 Td
(presented)Tj
4.1159 0 Td
(several)Tj
-34.7298 -1.2982 Td
(problems)Tj
3.9741 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.4683 0 Td
(separation)Tj
4.3824 0 Td
(of)Tj
1.0148 0 Td
(HGA)Tj
2.3867 0 Td
(from)Tj
2.211 0 Td
(similar)Tj
2.9821 0 Td
(amino)Tj
2.8006 0 Td
(acids)Tj
2.228 0 Td
(such)Tj
2.0636 0 Td
(as)Tj
1.0148 0 Td
(leucine)Tj
3.0784 0 Td
(and)Tj
1.7291 0 Td
(isoleucine)Tj
-32.3657 -1.2983 Td
(that)Tj
1.7744 0 Td
(were)Tj
2.126 0 Td
(invariably)Tj
4.2066 0 Td
(present)Tj
3.1634 0 Td
(in)Tj
1.0318 0 Td
(samples,)Tj
3.6113 0 Td
(leading)Tj
3.1295 0 Td
(to)Tj
1.0261 0 Td
(compromised)Tj
5.7656 0 Td
(precision)Tj
3.9062 0 Td
(and)Tj
1.7291 0 Td
(accuracy.)Tj
3.9401 0 Td
(The)Tj
-35.4101 -1.2983 Td
(use)Tj
1.5306 0 Td
(of)Tj
1.0148 0 Td
(reverse)Tj
3.0501 0 Td
(phase,)Tj
2.7099 0 Td
(liquid)Tj
2.5625 0 Td
(chromatography)Tj
6.8768 0 Td
(and)Tj
1.7291 0 Td
(pre-column)Tj
4.955 0 Td
(derivatisation)Tj
5.6806 0 Td
(with)Tj
1.9955 0 Td
(o-phthaldial-)Tj
-32.1049 -1.3039 Td
(dehyde)Tj
3.101 0 Td
(\(OPA\))Tj
2.8913 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4684 0 Td
(presence)Tj
3.7133 0 Td
(of)Tj
1.0148 0 Td
(2-mercaptoethanol)Tj
7.8236 0 Td
(slightly)Tj
3.0897 0 Td
(improved)Tj
4.1046 0 Td
(the)Tj
1.474 0 Td
(technique's)Tj
4.7395 0 Td
(resolu-)Tj
-34.4577 -1.2982 Td
(tion)Tj
1.8424 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(22)Tj
0 g
(],)Tj
1.7348 0 Td
(however)Tj
3.6113 0 Td
(it)Tj
0.7881 0 Td
(was)Tj
1.7007 0 Td
(the)Tj
1.4684 0 Td
(use)Tj
1.5307 0 Td
(of)Tj
1.0148 0 Td
(phenylisothiocyanate)Tj
8.691 0 Td
(\(PITC\))Tj
3.0727 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.6519 0 Td
(derivatising)Tj
4.9096 0 Td
(agent)Tj
-33.766 -1.2983 Td
(that)Tj
1.7744 0 Td
(yielded)Tj
3.0954 0 Td
(the)Tj
1.4684 0 Td
(best)Tj
1.8085 0 Td
(results)Tj
2.8119 0 Td
(allowing)Tj
3.617 0 Td
(optimal)Tj
3.3108 0 Td
(separation)Tj
4.3767 0 Td
(of)Tj
1.0148 0 Td
(closely-related)Tj
5.9584 0 Td
(amino)Tj
2.7949 0 Td
(acids)Tj
2.2337 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
-34.2649 -1.3039 Td
(Further)Tj
3.2654 0 Td
(optimisation)Tj
5.3121 0 Td
(and)Tj
1.7291 0 Td
(validation)Tj
4.2066 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(proposed)Tj
3.9514 0 Td
(method)Tj
3.3279 0 Td
(by)Tj
1.1792 0 Td
(Ware)Tj
2.4151 0 Td
(\(2001\))Tj
2.8006 0 Td
(determined)Tj
4.8529 0 Td
(the)Tj
-35.5235 -1.2983 Td
(official)Tj
2.9366 0 Td
(use)Tj
1.5364 0 Td
(of)Tj
1.0148 0 Td
(this)Tj
1.6724 0 Td
(chromatographic)Tj
7.1149 0 Td
(method)Tj
3.3222 0 Td
(for)Tj
1.389 0 Td
(analysis)Tj
3.3278 0 Td
(of)Tj
1.0148 0 Td
(HGA)Tj
2.3868 0 Td
(in)Tj
1.0318 0 Td
(Ackee)Tj
2.6702 0 Td
(products)Tj
3.7587 0 Td
([)Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(25)Tj
0 g
(].)Tj
-34.9112 -1.2983 Td
(However,)Tj
4.0761 0 Td
(this)Tj
1.6725 0 Td
(method)Tj
3.3278 0 Td
(still)Tj
1.6384 0 Td
(had)Tj
1.7065 0 Td
(a)Tj
0.6519 0 Td
(high)Tj
2.0126 0 Td
(limit)Tj
2.1147 0 Td
(of)Tj
1.0148 0 Td
(quantification)Tj
5.8393 0 Td
(\(1.4)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(g/ml\).)Tj
4.6658 0 Td
(Since)Tj
2.3584 0 Td
(HGA)Tj
2.3868 0 Td
(is)Tj
0.8503 0 Td
(poten-)Tj
-34.3159 -1.3039 Td
(tially)Tj
2.1656 0 Td
(toxic)Tj
2.1827 0 Td
(at)Tj
0.9524 0 Td
(lower)Tj
2.4491 0 Td
(concentrations)Tj
6.1795 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(],)Tj
1.6044 0 Td
(several)Tj
2.931 0 Td
(authors)Tj
3.2485 0 Td
(have)Tj
2.0579 0 Td
(described)Tj
4.0535 0 Td
(new)Tj
1.8766 0 Td
(methodologies)Tj
6.1001 0 Td
(that)Tj
-35.8013 -1.2982 Td
(led)Tj
1.4173 0 Td
(to)Tj
1.0261 0 Td
(reductions)Tj
4.473 0 Td
(to)Tj
1.0262 0 Td
(this)Tj
1.6724 0 Td
(limit,)Tj
2.3471 0 Td
(by)Tj
1.1792 0 Td
(using)Tj
2.3924 0 Td
(liquid)Tj
2.5625 0 Td
(chromatography)Tj
6.8768 0 Td
(coupled)Tj
3.3902 0 Td
(with)Tj
2.0013 0 Td
(mass)Tj
2.194 0 Td
(spectrome-)Tj
-32.5585 -1.2983 Td
(try)Tj
1.3436 0 Td
(\(LC-MS\))Tj
3.821 0 Td
(technology,)Tj
4.8472 0 Td
(and)Tj
1.7292 0 Td
(derivatisation)Tj
5.6806 0 Td
(with)Tj
1.9955 0 Td
(dansyl)Tj
2.795 0 Td
(chloride)Tj
3.5149 0 Td
(to)Tj
1.0205 0 Td
(measure)Tj
3.583 0 Td
(HGA,)Tj
2.6135 0 Td
(as)Tj
1.0148 0 Td
(well)Tj
1.8198 0 Td
(as)Tj
-35.7786 -1.3039 Td
(other)Tj
2.347 0 Td
(downstream)Tj
5.193 0 Td
(metabolic)Tj
4.1499 0 Td
(analogues)Tj
4.1669 0 Td
(such)Tj
2.058 0 Td
(as)Tj
1.0148 0 Td
(methylenecyclopropyl-glyc)Tj
10.7772 0 Td
(in)Tj
1.0318 0 Td
(\(MCPG\))Tj
3.7304 0 Td
([)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2643 0 Td
(26)Tj
0 g
(].)Tj
-35.7333 -1.2983 Td
(Derivatisation)Tj
5.8846 0 Td
(is)Tj
0.8504 0 Td
(commonly)Tj
4.5468 0 Td
(used)Tj
2.0579 0 Td
(in)Tj
1.0375 0 Td
(chromatographic)Tj
7.1149 0 Td
(methods)Tj
3.685 0 Td
(as)Tj
1.0148 0 Td
(it)Tj
0.7824 0 Td
(can)Tj
1.627 0 Td
(reduce)Tj
2.9197 0 Td
(selectivity)Tj
-31.521 -1.2982 Td
(issues)Tj
2.5171 0 Td
(and)Tj
1.7348 0 Td
(improve)Tj
3.5773 0 Td
(stability)Tj
3.3562 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(separation)Tj
4.3824 0 Td
(from)Tj
2.2053 0 Td
(other)Tj
2.3471 0 Td
(closely-related)Tj
5.9584 0 Td
(compounds.)Tj
5.1873 0 Td
(Fur-)Tj
-34.4633 -1.2983 Td
(ther,)Tj
2.0635 0 Td
(it)Tj
0.7881 0 Td
(improves)Tj
3.9401 0 Td
(detection,)Tj
4.1612 0 Td
(by)Tj
1.1849 0 Td
(increasing)Tj
4.3313 0 Td
(the)Tj
1.4684 0 Td
(signal-to-noise)Tj
6.1511 0 Td
(response)Tj
3.736 0 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(29)Tj
0 g
(].)Tj
3.5433 0 Td
(However,)Tj
4.0762 0 Td
(it)Tj
-35.4441 -1.3039 Td
(also)Tj
1.7687 0 Td
(increases)Tj
3.8325 0 Td
(cost)Tj
1.8085 0 Td
(and)Tj
1.7291 0 Td
(considerably)Tj
5.2951 0 Td
(lengthens)Tj
4.0591 0 Td
(sample)Tj
3.0218 0 Td
(preparation)Tj
4.9095 0 Td
(time)Tj
2.0296 0 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
2.075 0 Td
(Here)Tj
2.2053 0 Td
(we)Tj
1.3323 0 Td
(describe)Tj
-34.0665 -1.2983 Td
(a)Tj
0.652 0 Td
(novel)Tj
2.398 0 Td
(method)Tj
3.3278 0 Td
(using)Tj
2.3868 0 Td
(LC-MS)Tj
3.1408 0 Td
(that)Tj
1.7801 0 Td
(yields)Tj
2.5002 0 Td
(good)Tj
2.228 0 Td
(results)Tj
2.8063 0 Td
(when)Tj
2.4037 0 Td
(analysing)Tj
3.9798 0 Td
(plant)Tj
2.2677 0 Td
(material)Tj
3.5093 0 Td
(without)Tj
-33.3805 -1.2983 Td
(the)Tj
1.4683 0 Td
(use)Tj
1.5307 0 Td
(of)Tj
1.0148 0 Td
(any)Tj
1.6611 0 Td
(derivatising)Tj
4.9039 0 Td
(agent)Tj
2.3867 0 Td
(and)Tj
1.7348 0 Td
(with)Tj
1.9956 0 Td
(an)Tj
1.2019 0 Td
(improved)Tj
4.1102 0 Td
(quantification)Tj
5.8337 0 Td
(limit)Tj
2.1203 0 Td
(of)Tj
1.0147 0 Td
(0.5)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(g/g.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 414.7086 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Methods)Tj
/F13 1 Tf
0 -1.5023 Td
(Materials)Tj
4.1527 0 Td
(and)Tj
1.7953 0 Td
(standards)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 379.6724 cm
BT
/F7 1 Tf
1 TL
0.01 Tc
0 0 Td
(Hypoglycin)Tj
5.0116 0 Td
(A)Tj
0.9184 0 Td
(\(purity)Tj
/F14 1 Tf
0 Tc
3.0954 0 Td
(>)Tj
/F7 1 Tf
0.01 Tc
(95%\))Tj
3.0954 0 Td
(was)Tj
1.7405 0 Td
(obtained)Tj
3.8437 0 Td
(from)Tj
2.2621 0 Td
(Toronto)Tj
3.668 0 Td
(Chemicals)Tj
4.5127 0 Td
(\(Toronto,)Tj
4.2576 0 Td
(Canada\))Tj
-32.4054 -1.2982 Td
(while)Tj
2.4377 0 Td
(3-fluorovaline)Tj
6.0605 0 Td
(was)Tj
1.7404 0 Td
(obtained)Tj
3.8495 0 Td
(from)Tj
2.262 0 Td
(Apollo)Tj
3.0104 0 Td
(Chemicals)Tj
4.5127 0 Td
(\(Stockport,)Tj
4.8302 0 Td
(UK\).)Tj
2.2507 0 Td
(LCMS)Tj
2.846 0 Td
(Optima)Tj
-33.8001 -1.2983 Td
(grade)Tj
2.5114 0 Td
(solvents)Tj
3.532 0 Td
(used)Tj
2.1146 0 Td
(for)Tj
1.4287 0 Td
(buffers)Tj
3.084 0 Td
(were)Tj
2.177 0 Td
(purchased)Tj
4.4617 0 Td
(from)Tj
2.2621 0 Td
(Fisher)Tj
2.7836 0 Td
(Scientific)Tj
4.0365 0 Td
(\(Loughborough,)Tj
6.9618 0 Td
(UK\).)Tj
-35.3534 -1.2982 Td
(HPLC)Tj
2.8006 0 Td
(grade)Tj
2.5114 0 Td
(water)Tj
2.5059 0 Td
(for)Tj
1.4229 0 Td
(dissolution)Tj
4.7962 0 Td
(and)Tj
1.7688 0 Td
(methanol)Tj
4.1386 0 Td
(for)Tj
1.4286 0 Td
(extraction)Tj
4.3937 0 Td
(was)Tj
1.7461 0 Td
(purchased)Tj
4.4561 0 Td
(from)Tj
2.262 0 Td
(VWR)Tj
-34.2309 -1.304 Td
(\(Lutterworth,)Tj
5.8109 0 Td
(UK\).)Tj
2.2507 0 Td
(Individual)Tj
4.5014 0 Td
(stock)Tj
2.3754 0 Td
(solutions)Tj
3.9685 0 Td
(\(1mg/ml\))Tj
4.1726 0 Td
(of)Tj
1.0431 0 Td
(HGA)Tj
2.4265 0 Td
(and)Tj
1.7745 0 Td
(3-fluorovaline)Tj
6.0604 0 Td
(were)Tj
-34.384 -1.2982 Td
(prepared)Tj
3.9174 0 Td
(by)Tj
1.2076 0 Td
(dissolving)Tj
4.3653 0 Td
(1mg)Tj
2.0239 0 Td
(of)Tj
1.0432 0 Td
(each)Tj
2.0862 0 Td
(compound)Tj
4.7055 0 Td
(in)Tj
1.0658 0 Td
(1ml)Tj
1.8029 0 Td
(of)Tj
1.0431 0 Td
(HPLC-grade)Tj
5.4595 0 Td
(water.)Tj
2.7439 0 Td
(The)Tj
1.8198 0 Td
(standard)Tj
-33.2841 -1.2983 Td
(working)Tj
3.6566 0 Td
(solutions)Tj
3.9685 0 Td
(were)Tj
2.177 0 Td
(obtained)Tj
3.8494 0 Td
(by)Tj
1.2076 0 Td
(diluting)Tj
3.4809 0 Td
(the)Tj
1.508 0 Td
(stock)Tj
2.3754 0 Td
(solution)Tj
3.5943 0 Td
(at)Tj
0.9808 0 Td
(the)Tj
1.5137 0 Td
(concentration)Tj
6.0038 0 Td
(needed)Tj
-34.316 -1.3039 Td
(with)Tj
2.0522 0 Td
(water)Tj
2.5002 0 Td
(and)Tj
1.7744 0 Td
(stored)Tj
2.7893 0 Td
(at)Tj
0.9808 0 Td
(4C.)Tj
2.0353 0 Td
(Natural)Tj
3.3618 0 Td
(hazelnuts)Tj
4.1272 0 Td
(\()Tj
/F8 1 Tf
[( )]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.4514 0 Td
[()22(\n)3()15()4()4()16()6()]TJ
/F7 1 Tf
(\),)Tj
4.2066 0 Td
(obtained)Tj
3.8437 0 Td
(from)Tj
2.262 0 Td
(a)Tj
0.669 0 Td
(local)Tj
2.1487 0 Td
(market,)Tj
-34.2026 -1.2983 Td
(were)Tj
2.1769 0 Td
(used)Tj
2.1147 0 Td
(as)Tj
1.0431 0 Td
(a)Tj
0.669 0 Td
(matrix-matched)Tj
6.8768 0 Td
(plant)Tj
2.3357 0 Td
(material)Tj
3.6227 0 Td
(to)Tj
1.0545 0 Td
(perform)Tj
3.617 0 Td
(the)Tj
1.508 0 Td
(validation.)Tj
4.5864 0 Td
(Seeds)Tj
2.5001 0 Td
(and)Tj
1.7745 0 Td
(plant)Tj
-33.8794 -1.2982 Td
(materials)Tj
3.9968 0 Td
(tested)Tj
2.6362 0 Td
(from)Tj
2.262 0 Td
(other)Tj
2.4208 0 Td
(species)Tj
3.0954 0 Td
(were)Tj
2.177 0 Td
(obtained)Tj
3.8437 0 Td
(from)Tj
2.2621 0 Td
(local)Tj
2.1486 0 Td
(pastures)Tj
3.6227 0 Td
(and)Tj
1.7745 0 Td
(parkland)Tj
3.9061 0 Td
(by)Tj
1.2132 0 Td
(the)Tj
-35.3591 -1.304 Td
(researchers.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 217.0204 cm
BT
/F13 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Sample)Tj
3.2787 0 Td
(preparation)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 200.0125 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(T)Tj
0.01 Tc
(wenty-five)Tj
5.1646 0 Td
(grams)Tj
2.7383 0 Td
(of)Tj
1.0431 0 Td
(hazelnuts)Tj
4.1273 0 Td
(were)Tj
2.1769 0 Td
(homogenised)Tj
5.7543 0 Td
(to)Tj
1.0489 0 Td
(a)Tj
0.6689 0 Td
(fine)Tj
1.8028 0 Td
(powder)Tj
3.3336 0 Td
(with)Tj
2.0579 0 Td
(a)Tj
0.6633 0 Td
(500W)Tj
2.6702 0 Td
(grinder)Tj
-33.2501 -1.3039 Td
(device)Tj
2.8232 0 Td
(\(Tefal-Minipro\).)Tj
7.0753 0 Td
(Then)Tj
2.3867 0 Td
(HGA)Tj
2.4265 0 Td
(was)Tj
1.7404 0 Td
(added)Tj
2.7326 0 Td
(to)Tj
1.0545 0 Td
(exactly)Tj
3.0614 0 Td
(1g)Tj
1.1905 0 Td
(of)Tj
1.0432 0 Td
(seed)Tj
2.0069 0 Td
(homogenate)Tj
5.3178 0 Td
(at)Tj
0.9807 0 Td
(the)Tj
-33.8397 -1.2982 Td
(appropriate)Tj
5.0286 0 Td
(concentration)Tj
6.0037 0 Td
(\(calibration)Tj
5.0287 0 Td
(curve)Tj
2.4944 0 Td
(standards)Tj
4.2066 0 Td
(0.525)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(g;)Tj
4.1896 0 Td
(validation)Tj
4.3426 0 Td
(controls)Tj
-31.2942 -1.2983 Td
(0.8;8;24)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(g;)Tj
4.6261 0 Td
(blanks)Tj
2.897 0 Td
(0)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(g\).)Tj
2.3017 0 Td
(Validation)Tj
4.5807 0 Td
(control)Tj
3.2202 0 Td
(samples)Tj
3.4809 0 Td
(were)Tj
2.1827 0 Td
(prepared)Tj
3.9174 0 Td
(in)Tj
1.0602 0 Td
(triplicate.)Tj
4.1499 0 Td
(4ml)Tj
1.8028 0 Td
(of)Tj
-34.2196 -1.2983 Td
(methanol)Tj
4.1328 0 Td
(\(MeOH\))Tj
3.8154 0 Td
(was)Tj
1.7405 0 Td
(added)Tj
2.7326 0 Td
(to)Tj
1.0545 0 Td
(each)Tj
2.0806 0 Td
(sample)Tj
3.1067 0 Td
(and)Tj
1.7745 0 Td
(heated)Tj
2.9367 0 Td
(for)Tj
1.4286 0 Td
(1h)Tj
1.2529 0 Td
(at)Tj
0.9808 0 Td
(50C.)Tj
2.5228 0 Td
(Samples)Tj
3.5944 0 Td
(were)Tj
2.177 0 Td
(sub-)Tj
-35.3308 -1.3039 Td
(sequently)Tj
4.1328 0 Td
(spun)Tj
2.2337 0 Td
(at)Tj
0.9808 0 Td
(4800G)Tj
2.9083 0 Td
(for)Tj
1.4287 0 Td
(15)Tj
1.1962 0 Td
(min)Tj
1.8992 0 Td
(and)Tj
1.7688 0 Td
(the)Tj
1.5137 0 Td
(supernatant)Tj
5.1363 0 Td
(transferred)Tj
4.7906 0 Td
(to)Tj
1.0544 0 Td
(clean)Tj
2.3641 0 Td
(tubes)Tj
2.4095 0 Td
(and)Tj
-33.8171 -1.2982 Td
(evaporated)Tj
4.7508 0 Td
(at)Tj
0.9808 0 Td
(50C)Tj
2.279 0 Td
(under)Tj
2.6929 0 Td
(a)Tj
0.6633 0 Td
(nitrogen)Tj
3.7644 0 Td
(stream.)Tj
3.2485 0 Td
(The)Tj
1.8255 0 Td
(pellet)Tj
2.4604 0 Td
(was)Tj
1.7405 0 Td
(reconstituted)Tj
5.6749 0 Td
(with)Tj
2.058 0 Td
(100)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(l)Tj
2.4661 0 Td
(inter-)Tj
-34.6051 -1.2983 Td
(nal)Tj
1.491 0 Td
(standard)Tj
3.8324 0 Td
(solution)Tj
3.5943 0 Td
(\(3-fluorovaline)Tj
6.4176 0 Td
(at)Tj
0.9808 0 Td
(5)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(g/ml)Tj
3.1464 0 Td
(in)Tj
1.0658 0 Td
(H)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 413.1212 107.3196 cm
BT
/F7 1 Tf
1 TL
0.0133 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 416.8062 109.0204 cm
BT
/F7 1 Tf
1 TL
0.01 Tc
0 0 Td
(O\))Tj
1.3322 0 Td
(and)Tj
1.7745 0 Td
(900ul)Tj
2.4888 0 Td
(of)Tj
1.0431 0 Td
(deionised)Tj
4.201 0 Td
(water)Tj
2.5001 0 Td
(\(DI\))Tj
-35.0189 -1.3039 Td
(of)Tj
1.0432 0 Td
(LC-MS)Tj
3.2088 0 Td
(grade.)Tj
2.7495 0 Td
(The)Tj
1.8255 0 Td
(samples)Tj
3.481 0 Td
(were)Tj
2.177 0 Td
(then)Tj
2.0749 0 Td
(mixed)Tj
2.795 0 Td
(by)Tj
1.2075 0 Td
(vortex)Tj
2.8232 0 Td
(for)Tj
1.4286 0 Td
(30)Tj
1.2019 0 Td
(seconds)Tj
3.4696 0 Td
(before)Tj
2.8233 0 Td
(transfer)Tj
3.4242 0 Td
(to)Tj
-35.7332 -1.2982 Td
(glass)Tj
2.1543 0 Td
(HPLC)Tj
2.8063 0 Td
(vials.)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 357.6755 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Detection)Tj
4.3724 0 Td
(of)Tj
1.0418 0 Td
(hypoglyc)Tj
3.8693 0 Td
(in)Tj
0.985 0 Td
(A)Tj
0.8788 0 Td
(in)Tj
0.9779 0 Td
(plant)Tj
2.3457 0 Td
(material)Tj
3.7064 0 Td
(without)Tj
3.3307 0 Td
(derivatisa)Tj
4.1953 0 Td
(tion)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01995)Tj
4.089 0 Td
(21)Tj
0 g
2.0906 0 Td
(July)Tj
1.9701 0 Td
(3,)Tj
1.0417 0 Td
(2018)Tj
35.894 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(11)Tj
/F10 1 Tf
-0.025 Tc
-66.402 84.0904 Td
(design,)Tj
2.8984 0 Td
(data)Tj
1.7929 0 Td
(collection)Tj
3.7418 0 Td
(and)Tj
1.5874 0 Td
(analysis,)Tj
3.4087 0 Td
(decision)Tj
3.2953 0 Td
(to)Tj
-16.7245 -1.3748 Td
(publish,)Tj
3.1464 0 Td
(or)Tj
0.9993 0 Td
(preparation)Tj
4.4433 0 Td
(of)Tj
0.9496 0 Td
(the)Tj
1.3677 0 Td
(manuscript.)Tj
/F9 1 Tf
-10.9063 -2.126 Td
(Competing)Tj
4.3795 0 Td
(interests)Tj
3.3307 0 Td
(:)Tj
/F10 1 Tf
0.4819 0 Td
(The)Tj
1.5875 0 Td
(Comparat)Tj
3.6283 0 Td
(ive)Tj
-13.4079 -1.3749 Td
(Neuromusc)Tj
4.3299 0 Td
(ular)Tj
1.6158 0 Td
(Diseases)Tj
3.5291 0 Td
(Laboratory)Tj
4.252 0 Td
(receives)Tj
-13.7268 -1.3748 Td
(payment)Tj
3.437 0 Td
(for)Tj
1.2614 0 Td
(measureme)Tj
4.3937 0 Td
(nt)Tj
0.9497 0 Td
(of)Tj
0.9496 0 Td
(HGA)Tj
1.9205 0 Td
(in)Tj
0.8858 0 Td
(plant)Tj
2.0339 0 Td
(material)Tj
-15.8316 -1.3748 Td
(and)Tj
1.5874 0 Td
(proceeds)Tj
3.6354 0 Td
(contribute)Tj
3.9686 0 Td
(to)Tj
0.9496 0 Td
(ongoing)Tj
3.2528 0 Td
(laboratory)Tj
-13.3938 -1.3748 Td
(research.)Tj
3.6425 0 Td
(Howev)Tj
2.537 0 Td
(er,)Tj
1.1764 0 Td
(this)Tj
1.5662 0 Td
(does)Tj
2.0055 0 Td
(not)Tj
1.4244 0 Td
(alter)Tj
1.8142 0 Td
(our)Tj
-14.1662 -1.3748 Td
(adherence)Tj
4.0535 0 Td
(to)Tj
0.9496 0 Td
(PLOS)Tj
2.3316 0 Td
(ONE)Tj
1.8708 0 Td
(policies)Tj
3.0119 0 Td
(on)Tj
1.1622 0 Td
(sharing)Tj
2.9835 0 Td
(data)Tj
-16.3631 -1.3748 Td
(and)Tj
1.5874 0 Td
(materials.)Tj
3.8339 0 Td
(Further,)Tj
3.1039 0 Td
(one)Tj
1.5874 0 Td
(or)Tj
0.9992 0 Td
(more)Tj
2.1757 0 Td
(authors)Tj
3.0472 0 Td
(are)Tj
-16.3347 -1.3748 Td
(affiliated)Tj
3.3023 0 Td
(with)Tj
1.7788 0 Td
(the)Tj
1.3748 0 Td
(London)Tj
3.0614 0 Td
(Bioanaly)Tj
3.1182 0 Td
(sis)Tj
1.2614 0 Td
(Centre.)Tj
2.8418 0 Td
(This)Tj
-16.7387 -1.3749 Td
(does)Tj
2.0126 0 Td
(not)Tj
1.4173 0 Td
(alter)Tj
1.8213 0 Td
(our)Tj
1.474 0 Td
(adherence)Tj
4.0536 0 Td
(to)Tj
0.9496 0 Td
(PLOS)Tj
2.3315 0 Td
(ONE)Tj
1.8709 0 Td
(policies)Tj
-15.9308 -1.3748 Td
(on)Tj
1.1693 0 Td
(sharing)Tj
2.9834 0 Td
(data)Tj
1.7859 0 Td
(and)Tj
1.5945 0 Td
(material)Tj
ET
endstream
endobj
36 0 obj
<>stream
endstream
endobj
37 0 obj
<>stream
endstream
endobj
38 0 obj
<>stream
endstream
endobj
39 0 obj
<>stream
endstream
endobj
40 0 obj
<>stream
endstream
endobj
41 0 obj
<>stream
endstream
endobj
42 0 obj
<>stream
endstream
endobj
43 0 obj
<>stream
endstream
endobj
44 0 obj
<>stream
endstream
endobj
45 0 obj
<>stream
endstream
endobj
46 0 obj
<>stream
endstream
endobj
47 0 obj
<>stream
endstream
endobj
48 0 obj
<>stream
endstream
endobj
49 0 obj
<>stream
endstream
endobj
50 0 obj
<>stream
endstream
endobj
51 0 obj
<>stream
endstream
endobj
52 0 obj
<>stream
BT
10.4953 68.2162 Td
(s.)Tj
ET
Q
endstream
endobj
53 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 15 0 R/Annots 56 0 R/Contents 57 0 R/TrimBox[0 0 612 792]>>
endobj
56 0 obj
[58 0 R 59 0 R 60 0 R 61 0 R 62 0 R 63 0 R 64 0 R]
endobj
58 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref009)>>
endobj
59 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref026)>>
endobj
60 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref030)>>
endobj
61 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref031)>>
endobj
62 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref032)>>
endobj
63 0 obj
<>/Border[0 0 0]/Dest(Rpone.0199521.ref033)>>
endobj
64 0 obj
<>/Border[0 0 0]/A 65 0 R>>
endobj
65 0 obj
<>
endobj
57 0 obj
[66 0 R 67 0 R 68 0 R 69 0 R 70 0 R 71 0 R 72 0 R 73 0 R 74 0 R 75 0 R 76 0 R]
endobj
66 0 obj
<>stream
q
0.83 0.64 0.02 0 k
283.6913 410.0031 m
288.4535 410.0031 l
h
f*
292.9323 410.0031 m
302.4 410.0031 l
h
f*
306.8787 410.0031 m
316.4031 410.0031 l
h
f*
320.8252 410.0031 m
330.3496 410.0031 l
h
f*
273.8835 152.9008 m
283.4079 152.9008 l
h
f*
441.3543 152.9008 m
450.8787 152.9008 l
h
f*
0 g
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 706.0535 cm
BT
/F13 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(LC)Tj
/F15 1 Tf
[()]TJ
/F13 1 Tf
(MS/MS)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 689.1023 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(HGA)Tj
2.3867 0 Td
(concentrations)Tj
6.1795 0 Td
(in)Tj
1.0375 0 Td
(spiked)Tj
2.8119 0 Td
(hazelnut)Tj
3.6397 0 Td
(homogenates)Tj
5.5332 0 Td
(were)Tj
2.1259 0 Td
(determined)Tj
4.8473 0 Td
(on)Tj
1.2755 0 Td
(an)Tj
1.2019 0 Td
(LCMS-8040)Tj
5.0457 0 Td
(tri-)Tj
-36.0848 -1.3039 Td
(ple)Tj
1.4059 0 Td
(quadrupole)Tj
4.8076 0 Td
(instrument)Tj
4.6884 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
0.652 0 Td
(Nexera)Tj
3.0727 0 Td
(LC)Tj
1.423 0 Td
(front)Tj
2.2337 0 Td
(end)Tj
1.7178 0 Td
(\(Shimadzu)Tj
4.5524 0 Td
(UK,)Tj
1.8595 0 Td
(Milton)Tj
2.9763 0 Td
(Keynes\))Tj
3.447 0 Td
(using)Tj
-34.8319 -1.2982 Td
(positive-mode)Tj
5.9413 0 Td
(ESI.)Tj
1.8312 0 Td
(Samples)Tj
3.4979 0 Td
(were)Tj
2.1203 0 Td
(injected)Tj
3.3902 0 Td
(at)Tj
0.9525 0 Td
(1)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(L)Tj
1.7404 0 Td
(volumes)Tj
3.5603 0 Td
(onto)Tj
2.0807 0 Td
(a)Tj
0.6519 0 Td
(Phenomenex)Tj
5.4708 0 Td
(Kinetex)Tj
3.3166 0 Td
(2.6)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
-34.5541 -1.2983 Td
(HILIC)Tj
2.8686 0 Td
(100A)Tj
2.3301 0 Td
(\(50)Tj
1.5136 0 Td
(x)Tj
0.6804 0 Td
(2.1mm\))Tj
3.3788 0 Td
(column)Tj
3.2939 0 Td
(at)Tj
0.9524 0 Td
(25C)Tj
2.228 0 Td
(temperature.)Tj
5.3518 0 Td
(Mobile)Tj
3.0614 0 Td
(phases)Tj
2.846 0 Td
(consisted)Tj
3.9401 0 Td
(of)Tj
1.0148 0 Td
(2mM)Tj
-33.4599 -1.3039 Td
(ammonium)Tj
4.9605 0 Td
(formate)Tj
3.3619 0 Td
(\(pH3\))Tj
2.6646 0 Td
(in)Tj
1.0318 0 Td
(DI)Tj
1.2982 0 Td
(\(buffer)Tj
2.9651 0 Td
(A\))Tj
1.2415 0 Td
(and)Tj
1.7348 0 Td
(0.1%)Tj
2.1543 0 Td
(Optima)Tj
3.2995 0 Td
(grade)Tj
2.4435 0 Td
(formic)Tj
2.897 0 Td
(acid)Tj
1.8708 0 Td
(in)Tj
1.0375 0 Td
(Optima)Tj
-32.961 -1.2983 Td
(grade)Tj
2.4377 0 Td
(acetonitrile)Tj
4.7339 0 Td
(\(buffer)Tj
2.965 0 Td
(B\).)Tj
1.3606 0 Td
(A)Tj
0.9071 0 Td
(gradient)Tj
3.5546 0 Td
(was)Tj
1.7008 0 Td
(delivered)Tj
3.8834 0 Td
(at)Tj
0.9525 0 Td
(0.4ml/min)Tj
4.4276 0 Td
(starting)Tj
3.2655 0 Td
(from)Tj
2.2054 0 Td
(95%)Tj
1.9275 0 Td
(buffer)Tj
2.6249 0 Td
(B)Tj
-36.9465 -1.2982 Td
(for)Tj
1.3832 0 Td
(0.)Tj
0.9241 0 Td
(5)Tj
0.6917 0 Td
(min,)Tj
2.0806 0 Td
(followed)Tj
3.651 0 Td
(by)Tj
1.1849 0 Td
(a)Tj
0.6519 0 Td
(decrease)Tj
3.6057 0 Td
(to)Tj
1.0261 0 Td
(50%)Tj
1.9276 0 Td
(over)Tj
1.9785 0 Td
(2)Tj
0.6974 0 Td
(mins,)Tj
2.4377 0 Td
(held)Tj
1.9503 0 Td
(at)Tj
0.9524 0 Td
(50%)Tj
1.9275 0 Td
(for)Tj
1.3833 0 Td
(1)Tj
0.6974 0 Td
(min,)Tj
2.0806 0 Td
(then)Tj
2.0182 0 Td
(reduced)Tj
3.4469 0 Td
(to)Tj
-36.697 -1.304 Td
(30%)Tj
1.9275 0 Td
(buffer)Tj
2.6249 0 Td
(B)Tj
0.7993 0 Td
(over)Tj
1.9786 0 Td
(0.1min)Tj
3.033 0 Td
(and)Tj
1.7292 0 Td
(held)Tj
1.9445 0 Td
(at)Tj
0.9525 0 Td
(30%)Tj
1.9275 0 Td
(for)Tj
1.389 0 Td
(1)Tj
0.6916 0 Td
(min)Tj
1.8539 0 Td
(before)Tj
2.7439 0 Td
(returning)Tj
4.0422 0 Td
(to)Tj
1.0261 0 Td
(95%)Tj
1.9276 0 Td
(buffer)Tj
2.6248 0 Td
(B)Tj
0.7994 0 Td
(over)Tj
1.9786 0 Td
(0.1)Tj
-35.9941 -1.2982 Td
(min)Tj
1.8538 0 Td
(for)Tj
1.389 0 Td
(re-equilibration)Tj
6.5366 0 Td
(for)Tj
1.3833 0 Td
(2.3mins.)Tj
3.617 0 Td
(The)Tj
1.7858 0 Td
(following)Tj
3.9798 0 Td
(optimised)Tj
4.2066 0 Td
(instrument)Tj
4.6828 0 Td
(parameters)Tj
4.6772 0 Td
(were)Tj
-34.1119 -1.2983 Td
(applied)Tj
3.1521 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.474 0 Td
(detection)Tj
3.9344 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(analytes:)Tj
3.651 0 Td
(nebulising)Tj
4.3766 0 Td
(gas)Tj
1.4854 0 Td
(at)Tj
0.9467 0 Td
(3L/min;)Tj
3.4243 0 Td
(drying)Tj
2.8573 0 Td
(gas)Tj
1.4796 0 Td
(at)Tj
0.9525 0 Td
(15L/min;)Tj
3.9004 0 Td
(heat)Tj
-35.5008 -1.2983 Td
(block)Tj
2.398 0 Td
(temperature)Tj
5.1307 0 Td
(at)Tj
0.9525 0 Td
(400C;)Tj
2.9253 0 Td
(desolvation)Tj
4.7962 0 Td
(line)Tj
1.7064 0 Td
(temperature)Tj
5.125 0 Td
(at)Tj
0.9525 0 Td
(250C;)Tj
2.931 0 Td
(Column)Tj
3.5319 0 Td
(oven)Tj
2.1543 0 Td
(at)Tj
0.9525 0 Td
(25C;)Tj
-33.5563 -1.3039 Td
(Autosampler)Tj
5.4084 0 Td
(temperature)Tj
5.1307 0 Td
(at)Tj
0.9524 0 Td
(10C.)Tj
2.4491 0 Td
(The)Tj
1.7859 0 Td
(complete)Tj
3.8777 0 Td
(system)Tj
2.9367 0 Td
(was)Tj
1.7008 0 Td
(controlled)Tj
4.32 0 Td
(by)Tj
1.1792 0 Td
(LabSolutions)Tj
5.4368 0 Td
(soft-)Tj
-35.1777 -1.2982 Td
(ware)Tj
2.1259 0 Td
(\(Shimadzu\),)Tj
5.1137 0 Td
(version)Tj
3.1521 0 Td
(5.65,)Tj
2.092 0 Td
(running)Tj
3.4922 0 Td
(on)Tj
1.2699 0 Td
(a)Tj
0.652 0 Td
(HP)Tj
1.5364 0 Td
(Prodesk)Tj
3.4639 0 Td
(computer)Tj
4.1045 0 Td
(with)Tj
1.9899 0 Td
(Windows)Tj
4.0762 0 Td
(7)Tj
0.6917 0 Td
(operating)Tj
-33.7604 -1.2983 Td
(system.)Tj
1.1962 -1.3039 Td
(Quantitation)Tj
5.3971 0 Td
(was)Tj
1.7008 0 Td
(determined)Tj
4.8529 0 Td
(by)Tj
1.1792 0 Td
(multiple)Tj
3.5659 0 Td
(reaction)Tj
3.4923 0 Td
(monitoring)Tj
4.8188 0 Td
(\(HGA)Tj
2.727 0 Td
(quantitation)Tj
5.159 0 Td
(ion)Tj
1.542 0 Td
(m/z)Tj
-35.6312 -1.2983 Td
(142.2)Tj
/F16 1 Tf
0 Tc
2.347 0 Td
(!)Tj
/F7 1 Tf
-0.004 Tc
1.2189 0 Td
(74.05,)Tj
2.5739 0 Td
(Dwell)Tj
2.5568 0 Td
(time)Tj
2.0296 0 Td
(100ms;)Tj
3.05 0 Td
(Q1)Tj
1.4457 0 Td
(Pre-bias)Tj
3.4923 0 Td
(at)Tj
0.9524 0 Td
(-15.0V;)Tj
3.1408 0 Td
(Collision)Tj
3.8324 0 Td
(Energy)Tj
3.0557 0 Td
(at)Tj
0.9524 0 Td
(-10V;)Tj
2.4435 0 Td
(Q3)Tj
1.44 0 Td
(Pre-)Tj
-34.5314 -1.2982 Td
(bias)Tj
1.7858 0 Td
(at)Tj
0.9524 0 Td
(-29.0V.)Tj
3.1464 0 Td
(FVal)Tj
2.1203 0 Td
(quantitation)Tj
5.1534 0 Td
(ion)Tj
1.5477 0 Td
(m/z)Tj
1.7801 0 Td
(136.2)Tj
/F16 1 Tf
0 Tc
2.3471 0 Td
(!)Tj
/F7 1 Tf
-0.004 Tc
1.2189 0 Td
(70.0,)Tj
2.0976 0 Td
(Dwell)Tj
2.5569 0 Td
(time)Tj
2.0296 0 Td
(100ms;)Tj
3.0557 0 Td
(Q1)Tj
1.44 0 Td
(Pre)Tj
1.576 0 Td
(bias)Tj
1.7858 0 Td
(-14.0V;)Tj
-34.5937 -1.304 Td
(Collision)Tj
3.8324 0 Td
(Energy)Tj
3.05 0 Td
(at)Tj
0.9525 0 Td
(-16.0V;)Tj
3.1464 0 Td
(Q3)Tj
1.44 0 Td
(Pre-bias)Tj
3.4923 0 Td
(at)Tj
0.9524 0 Td
(-28.0V.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 441.0708 cm
BT
/F13 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Optimisation)Tj
5.8394 0 Td
(of)Tj
1.0677 0 Td
(extraction)Tj
4.4646 0 Td
(technique)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 424.0629 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Methanol,)Tj
4.3199 0 Td
(aqueous)Tj
3.5093 0 Td
(ethanol)Tj
3.2031 0 Td
(and)Tj
1.7348 0 Td
(water)Tj
2.4321 0 Td
(extraction)Tj
4.252 0 Td
(have)Tj
2.0636 0 Td
(all)Tj
1.1452 0 Td
(been)Tj
2.1203 0 Td
(suggested)Tj
4.0762 0 Td
(as)Tj
1.0148 0 Td
(solvents)Tj
3.4185 0 Td
(of)Tj
1.0148 0 Td
(choice)Tj
-34.3046 -1.2982 Td
(for)Tj
1.3832 0 Td
(HGA)Tj
2.3868 0 Td
(extraction)Tj
4.2576 0 Td
([)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2643 0 Td
(26)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.3946 0 Td
(30)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.3946 0 Td
(31)Tj
0 g
(].)Tj
1.7405 0 Td
(Carlier)Tj
/F8 1 Tf
2.9934 0 Td
[()8()]TJ
0.9127 0 Td
[()11()]TJ
/F7 1 Tf
(.)Tj
1.1679 0 Td
(\(2015\))Tj
2.8006 0 Td
(also)Tj
1.7688 0 Td
(proposed)Tj
3.9515 0 Td
(the)Tj
1.4683 0 Td
(use)Tj
1.5364 0 Td
(of)Tj
1.0148 0 Td
(sonication)Tj
4.3766 0 Td
(to)Tj
-35.8126 -1.3039 Td
(improve)Tj
3.5773 0 Td
(and)Tj
1.7347 0 Td
(perhaps)Tj
3.3676 0 Td
(shorten)Tj
3.2598 0 Td
(the)Tj
1.4683 0 Td
(extraction)Tj
4.2577 0 Td
(time)Tj
2.0295 0 Td
(from)Tj
2.2054 0 Td
(ackee)Tj
2.4208 0 Td
(fruit.)Tj
2.1996 0 Td
(We)Tj
1.6044 0 Td
(tested)Tj
2.5569 0 Td
(3)Tj
0.6916 0 Td
(different)Tj
3.6567 0 Td
(sol-)Tj
-35.0303 -1.2983 Td
(vents)Tj
2.3073 0 Td
(\(methanol,)Tj
4.5865 0 Td
(ethanol)Tj
3.2088 0 Td
(and)Tj
1.7291 0 Td
(water\))Tj
2.7723 0 Td
(at)Tj
0.9524 0 Td
(50C)Tj
2.2223 0 Td
(for)Tj
1.389 0 Td
(1h)Tj
1.2246 0 Td
(on)Tj
1.2755 0 Td
(4)Tj
0.6917 0 Td
(different)Tj
3.6567 0 Td
(sycamore)Tj
4.0138 0 Td
(seed)Tj
1.9559 0 Td
(homogenates)Tj
-31.9859 -1.2983 Td
(and)Tj
1.7291 0 Td
(compared)Tj
4.2462 0 Td
(them)Tj
2.2848 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(extraction)Tj
4.2576 0 Td
(obtained)Tj
3.7361 0 Td
(on)Tj
1.2756 0 Td
(duplicate)Tj
3.8834 0 Td
(samples)Tj
3.3845 0 Td
(with)Tj
1.9956 0 Td
(the)Tj
1.4684 0 Td
(same)Tj
2.262 0 Td
(solvents)Tj
3.4186 0 Td
(at)Tj
-36.4363 -1.3039 Td
(room)Tj
2.4207 0 Td
(temperature)Tj
5.1307 0 Td
(\(20C\))Tj
2.9083 0 Td
(for)Tj
1.3833 0 Td
(24h.)Tj
1.9276 0 Td
(Results)Tj
3.0557 0 Td
(were)Tj
2.126 0 Td
(analysed)Tj
3.651 0 Td
(by)Tj
1.1792 0 Td
(ANOVA)Tj
3.7644 0 Td
(followed)Tj
3.651 0 Td
(by)Tj
1.1792 0 Td
(Fisher's)Tj
3.2768 0 Td
(LSD)Tj
-35.6539 -1.2982 Td
(multiple)Tj
3.5659 0 Td
(comparison)Tj
4.9776 0 Td
(test.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 319.011 cm
BT
/F13 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Evaluation)Tj
4.7244 0 Td
(of)Tj
1.0677 0 Td
(plant)Tj
2.4095 0 Td
(matrix)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 302.0031 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Seed)Tj
2.0692 0 Td
(homogenates)Tj
5.5332 0 Td
(were)Tj
2.126 0 Td
(evaluated)Tj
3.9855 0 Td
(as)Tj
1.0148 0 Td
(matrix-matched)Tj
6.684 0 Td
(material)Tj
3.515 0 Td
(to)Tj
1.0261 0 Td
(produce)Tj
3.5149 0 Td
(the)Tj
1.4684 0 Td
(calibration)Tj
4.524 0 Td
(stan-)Tj
-35.4611 -1.2982 Td
(dards)Tj
2.4377 0 Td
(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
(methodology.)Tj
5.7317 0 Td
(Seeds)Tj
2.4321 0 Td
(from)Tj
2.2053 0 Td
(field)Tj
1.9729 0 Td
(maple)Tj
2.6589 0 Td
(\()Tj
/F8 1 Tf
[(\r)]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.44 0 Td
[()7()16()15()4()8()9()7()20()]TJ
/F7 1 Tf
(,)Tj
4.6488 0 Td
(Norway)Tj
3.4072 0 Td
(maple)Tj
/F8 1 Tf
2.6589 0 Td
[(\r)]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.4286 0 Td
[()10()4()22()11()]TJ
-33.8794 -1.2983 Td
[()6()11()4()4()8()]TJ
/F7 1 Tf
(,)Tj
3.2428 0 Td
(Ash)Tj
1.7914 0 Td
(tree)Tj
/F8 1 Tf
1.7348 0 Td
[()25()20()4()11()-4()]TJ
3.9685 0 Td
[()1()-4()7()15()-4()10()4()11()]TJ
/F7 1 Tf
(,)Tj
4.0479 0 Td
(each)Tj
2.0296 0 Td
(that)Tj
1.7744 0 Td
(have)Tj
2.0636 0 Td
(seeds)Tj
2.3131 0 Td
(that)Tj
1.7802 0 Td
(are)Tj
1.4513 0 Td
(morphologically)Tj
6.7577 0 Td
(similar)Tj
2.9877 0 Td
(to)Tj
-35.943 -1.3039 Td
(those)Tj
2.3357 0 Td
(of)Tj
1.0148 0 Td
(sycamore)Tj
4.0138 0 Td
(trees)Tj
2.0976 0 Td
(\()Tj
/F8 1 Tf
[(\r)]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.44 0 Td
[()7()-3()2()4()4()11()10()4()22()11()16()18()-4()]TJ
/F7 1 Tf
(\))Tj
6.616 0 Td
(were)Tj
2.1204 0 Td
(tested)Tj
2.5511 0 Td
(alongside)Tj
4.0025 0 Td
(hazelnuts)Tj
3.9968 0 Td
(\()Tj
/F8 1 Tf
[( )]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.4117 0 Td
[()22(\n)3()15()4()4()16()6()]TJ
/F7 1 Tf
(\).)Tj
4.0648 0 Td
(Two)Tj
-35.6652 -1.2983 Td
(seed)Tj
1.9558 0 Td
(homogenates)Tj
5.5332 0 Td
(from)Tj
2.2054 0 Td
(2)Tj
0.6973 0 Td
(different)Tj
3.6567 0 Td
(trees)Tj
2.0976 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
2.0296 0 Td
(species,)Tj
3.2201 0 Td
(were)Tj
2.1203 0 Td
(spiked)Tj
2.8176 0 Td
(with)Tj
1.9956 0 Td
(10)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(g)Tj
2.143 0 Td
(HGA,)Tj
2.6135 0 Td
(then)Tj
-34.1005 -1.2982 Td
(extracted)Tj
3.8834 0 Td
(\(as)Tj
1.3549 0 Td
(above\).)Tj
3.1125 0 Td
(Recovery)Tj
3.9004 0 Td
(obtained)Tj
3.7304 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(4)Tj
0.6973 0 Td
(different)Tj
3.6567 0 Td
(seed)Tj
1.9559 0 Td
(matrices)Tj
3.617 0 Td
(was)Tj
1.7007 0 Td
(compared)Tj
4.2406 0 Td
(by)Tj
-34.3556 -1.2983 Td
(ANOVA)Tj
3.7643 0 Td
(using)Tj
2.3925 0 Td
(Dunnett's)Tj
4.1725 0 Td
(multiple)Tj
3.566 0 Td
(comparison)Tj
4.9776 0 Td
(test.)Tj
1.8255 0 Td
(Differences)Tj
4.7735 0 Td
(were)Tj
2.126 0 Td
(considered)Tj
4.5978 0 Td
(statistically)Tj
-32.1957 -1.3039 Td
(significantly)Tj
5.0853 0 Td
(different)Tj
3.6566 0 Td
(when)Tj
2.4038 0 Td
(p)Tj
/F14 1 Tf
0 Tc
(<)Tj
/F7 1 Tf
-0.004 Tc
(0.05.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 184.0251 cm
BT
/F13 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Evaluation)Tj
4.7244 0 Td
(criteria)Tj
3.2646 0 Td
(for)Tj
1.4693 0 Td
(validation)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 167.0173 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Criteria)Tj
3.3165 0 Td
(used)Tj
2.0579 0 Td
(in)Tj
1.0375 0 Td
(this)Tj
1.6724 0 Td
(work)Tj
2.279 0 Td
(followed)Tj
3.651 0 Td
(the)Tj
1.4684 0 Td
(National)Tj
3.6907 0 Td
(Measurement)Tj
5.7429 0 Td
(System)Tj
3.0558 0 Td
(Guidelines)Tj
4.524 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
-33.5109 -1.3039 Td
(United)Tj
3.0217 0 Td
(Kingdom)Tj
4.0251 0 Td
([)Tj
0.83 0.64 0.02 0 k
(32)Tj
0 g
(])Tj
1.8539 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(European)Tj
4.1216 0 Td
(Medicines)Tj
4.354 0 Td
(Agency)Tj
3.2201 0 Td
([)Tj
0.83 0.64 0.02 0 k
(33)Tj
0 g
(])Tj
1.8539 0 Td
(and)Tj
1.7291 0 Td
(are)Tj
1.4456 0 Td
(summarised)Tj
5.1364 0 Td
(below.)Tj
-33.9588 -1.2982 Td
(Validation)Tj
4.4446 0 Td
(was)Tj
1.6952 0 Td
(performed)Tj
4.473 0 Td
(over)Tj
1.9729 0 Td
(5)Tj
0.6973 0 Td
(days)Tj
1.9956 0 Td
(during)Tj
2.931 0 Td
(which)Tj
2.6475 0 Td
(calibration)Tj
4.5241 0 Td
(curves)Tj
2.7836 0 Td
(and)Tj
1.7291 0 Td
(validation)Tj
4.2066 0 Td
(controls)Tj
-34.1005 -1.2983 Td
(were)Tj
2.1203 0 Td
(freshly)Tj
2.8913 0 Td
(prepared)Tj
3.8097 0 Td
(3)Tj
0.6917 0 Td
(times)Tj
2.3924 0 Td
(on)Tj
1.2756 0 Td
(3)Tj
0.6973 0 Td
(different)Tj
3.651 0 Td
(days)Tj
2.0012 0 Td
(by)Tj
1.1792 0 Td
(2)Tj
0.6974 0 Td
(different)Tj
3.6566 0 Td
(analysts.)Tj
-25.0637 -1.8992 Td
(1.)Tj
1.2018 0 Td
(Accuracy:)Tj
4.1839 0 Td
(Calculated)Tj
4.4447 0 Td
(as)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(mean)Tj
2.4435 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(inter-day)Tj
3.8948 0 Td
(theoretical)Tj
4.4447 0 Td
(recovery)Tj
3.6453 0 Td
(at)Tj
0.9525 0 Td
(each)Tj
2.0296 0 Td
(concentra-)Tj
-31.0109 -1.3039 Td
(tion)Tj
1.8369 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(hazelnut)Tj
3.634 0 Td
(homogenate)Tj
5.176 0 Td
(validation)Tj
4.2066 0 Td
(control)Tj
3.1237 0 Td
(\(VC\))Tj
2.2564 0 Td
(samples,)Tj
3.6057 0 Td
(lower)Tj
2.4547 0 Td
(validation)Tj
4.201 0 Td
(control)Tj
-32.9838 -1.2983 Td
(\(LVC\),)Tj
3.0217 0 Td
(middle)Tj
3.0274 0 Td
(validation)Tj
4.2066 0 Td
(control)Tj
3.1238 0 Td
(\(MVC\))Tj
3.1464 0 Td
(and)Tj
1.7348 0 Td
(upper)Tj
2.5738 0 Td
(validation)Tj
4.2066 0 Td
(control)Tj
3.1238 0 Td
(\(UVC\).)Tj
3.2201 0 Td
(Accuracy)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 357.6755 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Detection)Tj
4.3724 0 Td
(of)Tj
1.0418 0 Td
(hypoglyc)Tj
3.8693 0 Td
(in)Tj
0.985 0 Td
(A)Tj
0.8788 0 Td
(in)Tj
0.9779 0 Td
(plant)Tj
2.3457 0 Td
(material)Tj
3.7064 0 Td
(without)Tj
3.3307 0 Td
(derivatisa)Tj
4.1953 0 Td
(tion)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01995)Tj
4.089 0 Td
(21)Tj
0 g
2.0906 0 Td
(July)Tj
1.9701 0 Td
(3,)Tj
1.0417 0 Td
(2018)Tj
35.894 0 Td
(3)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
67 0 obj
<>stream
endstream
endobj
68 0 obj
<>stream
endstream
endobj
69 0 obj
<>stream
endstream
endobj
70 0 obj
<>stream
endstream
endobj
71 0 obj
<>stream
endstream
endobj
72 0 obj
<>stream
endstream
endobj
73 0 obj
<>stream
endstream
endobj
74 0 obj
<>stream
endstream
endobj
75 0 obj
<>stream
endstream
endobj
76 0 obj
<>stream
BT
66.402 0 Td
(11)Tj
ET
Q
endstream
endobj
77 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 15 0 R/Annots 78 0 R/Contents 79 0 R/TrimBox[0 0 612 792]>>
endobj
78 0 obj
[80 0 R]
endobj
80 0 obj
<>/Border[0 0 0]/A 81 0 R>>
endobj
81 0 obj
<>
endobj
79 0 obj
<>stream
q
1 j
1 J
0 w
10 0 0 10 212.0314 707.4141 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(was)Tj
1.6951 0 Td
(considered)Tj
4.6034 0 Td
(acceptable)Tj
4.3483 0 Td
(if)Tj
0.7767 0 Td
(the)Tj
1.4683 0 Td
(recovery)Tj
3.651 0 Td
(was)Tj
1.7008 0 Td
(between)Tj
3.5263 0 Td
(80%)Tj
1.9275 0 Td
(and)Tj
1.7292 0 Td
(110%)Tj
2.4037 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(designated)Tj
-30.3191 -1.3039 Td
(HGA)Tj
2.3867 0 Td
(concentration.)Tj
-3.5885 -1.8992 Td
(2.)Tj
1.2018 0 Td
(Precision:)Tj
4.1725 0 Td
(calculated)Tj
4.2009 0 Td
(as)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(coefficient)Tj
4.3767 0 Td
(of)Tj
1.0148 0 Td
(variation)Tj
3.8041 0 Td
(\(CV\))Tj
2.2563 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(intra-day)Tj
3.9061 0 Td
(accuracy)Tj
3.7134 0 Td
(of)Tj
1.0148 0 Td
(forti-)Tj
-33.4316 -1.2982 Td
(fied)Tj
1.7234 0 Td
(hazelnut)Tj
3.6397 0 Td
(homogenate)Tj
5.176 0 Td
(VC)Tj
1.576 0 Td
(samples.)Tj
3.6057 0 Td
(Precision)Tj
3.9515 0 Td
(was)Tj
1.7007 0 Td
(considered)Tj
4.5978 0 Td
(acceptable)Tj
4.3483 0 Td
(if)Tj
0.7767 0 Td
(the)Tj
1.4684 0 Td
(percent)Tj
-32.5642 -1.2983 Td
(CVs)Tj
1.9388 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.4684 0 Td
(3)Tj
0.6973 0 Td
(repeated)Tj
3.6397 0 Td
(measurements)Tj
6.032 0 Td
(were)Tj
2.1203 0 Td
(less)Tj
1.6158 0 Td
(than)Tj
2.0239 0 Td
(15%,)Tj
2.1543 0 Td
(except)Tj
2.7723 0 Td
(at)Tj
0.9524 0 Td
(the)Tj
1.4684 0 Td
(LVC)Tj
2.1203 0 Td
(concentration)Tj
-30.3872 -1.3039 Td
(where)Tj
2.6475 0 Td
(it)Tj
0.788 0 Td
(could)Tj
2.4491 0 Td
(be)Tj
/F16 1 Tf
0 Tc
1.1509 0 Td
()Tj
/F7 1 Tf
-0.004 Tc
(20%.)Tj
-8.2373 -1.8992 Td
(3.)Tj
1.2018 0 Td
(Reproducibility:)Tj
6.6727 0 Td
(calculated)Tj
4.2009 0 Td
(as)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(CV)Tj
1.5817 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(inter-day)Tj
3.8948 0 Td
(accuracy)Tj
3.7133 0 Td
(of)Tj
1.0148 0 Td
(fortified)Tj
3.4583 0 Td
(hazelnut)Tj
3.6396 0 Td
(homog-)Tj
-33.1424 -1.2983 Td
(enate)Tj
2.347 0 Td
(VC)Tj
1.5761 0 Td
(samples.)Tj
3.6056 0 Td
(Reproducibility)Tj
6.4516 0 Td
(was)Tj
1.6951 0 Td
(considered)Tj
4.6035 0 Td
(acceptable)Tj
4.3483 0 Td
(if)Tj
0.7767 0 Td
(the)Tj
1.4683 0 Td
(percent)Tj
3.2258 0 Td
(CVs)Tj
1.9389 0 Td
(did)Tj
1.5421 0 Td
(not)Tj
-33.579 -1.2982 Td
(exceed)Tj
2.9026 0 Td
(the)Tj
1.474 0 Td
(level)Tj
2.0126 0 Td
(calculated)Tj
4.2066 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.4683 0 Td
(Horwitz)Tj
3.532 0 Td
(equation)Tj
3.7303 0 Td
(\(Hweq\))Tj
3.2825 0 Td
(CV)Tj
1.5761 0 Td
(=)Tj
0.7994 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 478.4314 595.8425 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(\(10.5logC\))Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 513.8078 591.4204 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(,)Tj
0.4478 0 Td
(where)Tj
2.6476 0 Td
(C)Tj
0.8844 0 Td
(is)Tj
0.8504 0 Td
(the)Tj
-35.0076 -1.3039 Td
(concentration)Tj
5.8167 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(analyte)Tj
3.0614 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.652 0 Td
(decimal)Tj
3.3618 0 Td
(fraction.)Tj
-17.5917 -1.8992 Td
(4.)Tj
1.2019 0 Td
(System)Tj
3.0501 0 Td
(Linearity:)Tj
4.0478 0 Td
(determined)Tj
4.8529 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.474 0 Td
(evaluation)Tj
4.3597 0 Td
(of)Tj
1.0148 0 Td
(3)Tj
0.6916 0 Td
(HGA)Tj
2.3924 0 Td
(standard)Tj
3.7191 0 Td
(curves)Tj
2.7836 0 Td
(prepared)Tj
3.8039 0 Td
(and)Tj
-33.3691 -1.2982 Td
(analysed)Tj
3.6453 0 Td
(on)Tj
1.2756 0 Td
(3)Tj
0.6973 0 Td
(separate)Tj
3.481 0 Td
(days.)Tj
2.2223 0 Td
(Each)Tj
2.177 0 Td
(standard)Tj
3.7247 0 Td
(curve)Tj
2.4208 0 Td
(consisted)Tj
3.9344 0 Td
(of)Tj
1.0148 0 Td
(8)Tj
0.6974 0 Td
(standards)Tj
4.0818 0 Td
(ranging)Tj
3.3221 0 Td
(from)Tj
-32.6945 -1.2983 Td
(0.1)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(g/ml)Tj
3.7644 0 Td
(to)Tj
1.0205 0 Td
(25)Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(g/ml)Tj
3.5432 0 Td
(equivalent)Tj
4.3484 0 Td
(concentration.)Tj
6.0434 0 Td
(For)Tj
1.6271 0 Td
(each)Tj
2.0296 0 Td
(validation)Tj
4.2065 0 Td
(run,)Tj
1.8936 0 Td
(the)Tj
1.4683 0 Td
(resulting)Tj
3.7246 0 Td
(HGA)Tj
-33.6696 -1.3039 Td
(standard)Tj
3.719 0 Td
(curve)Tj
2.4208 0 Td
(was)Tj
1.7008 0 Td
(expected)Tj
3.7247 0 Td
(to)Tj
1.0204 0 Td
(meet)Tj
2.1884 0 Td
(the)Tj
1.4683 0 Td
(following)Tj
3.9798 0 Td
(conditions:)Tj
-20.1939 -2.0013 Td
(a.)Tj
1.1622 0 Td
(The)Tj
1.7802 0 Td
(deviation)Tj
3.9458 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(calculated)Tj
4.2009 0 Td
(concentration)Tj
5.8223 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
2.0296 0 Td
(standard)Tj
3.7191 0 Td
(should)Tj
2.9196 0 Td
(be)Tj
1.1508 0 Td
(within)Tj
/F12 1 Tf
2.8119 0 Td
[()]TJ
/F7 1 Tf
(15%)Tj
2.5002 0 Td
(of)Tj
-34.384 -1.2982 Td
(the)Tj
1.4684 0 Td
(theoretical)Tj
4.439 0 Td
(value)Tj
2.3074 0 Td
(\()Tj
/F12 1 Tf
[()]TJ
/F7 1 Tf
(20%)Tj
2.8346 0 Td
(at)Tj
0.9525 0 Td
(LLOQ\).)Tj
-13.2378 -1.8992 Td
(b.)Tj
1.2359 0 Td
(Individual)Tj
4.3597 0 Td
(standards)Tj
4.0819 0 Td
(\()Tj
/F16 1 Tf
0 Tc
()Tj
/F7 1 Tf
-0.004 Tc
(1/3\))Tj
2.9536 0 Td
(could)Tj
2.4548 0 Td
(be)Tj
1.1452 0 Td
(dropped)Tj
3.617 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.4683 0 Td
(curve)Tj
2.4208 0 Td
(if)Tj
0.7767 0 Td
(they)Tj
1.9218 0 Td
(did)Tj
1.5421 0 Td
(not)Tj
1.5759 0 Td
(meet)Tj
2.1827 0 Td
(these)Tj
-32.7059 -1.2983 Td
(criteria,)Tj
3.2996 0 Td
(although)Tj
3.753 0 Td
(no)Tj
1.2756 0 Td
(quantitation)Tj
5.159 0 Td
(should)Tj
2.914 0 Td
(be)Tj
1.1509 0 Td
(extrapolated)Tj
5.1647 0 Td
(outside)Tj
3.1351 0 Td
(the)Tj
1.4683 0 Td
(range)Tj
2.4603 0 Td
(covered)Tj
3.3562 0 Td
(by)Tj
-33.1367 -1.3039 Td
(the)Tj
1.4684 0 Td
(acceptable)Tj
4.3483 0 Td
(standards.)Tj
-8.2091 -1.8992 Td
(5.)Tj
1.2019 0 Td
(The)Tj
1.7801 0 Td
(Lower)Tj
2.744 0 Td
(Limit)Tj
2.4094 0 Td
(of)Tj
1.0148 0 Td
(Detection)Tj
4.1499 0 Td
(\(LOD\):)Tj
3.1464 0 Td
(determined)Tj
4.8472 0 Td
(from)Tj
2.2054 0 Td
(6)Tj
0.6973 0 Td
(blanks)Tj
2.812 0 Td
(according)Tj
4.1895 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(following)Tj
-32.4904 -1.2983 Td
(formula:)Tj
3.6397 0 Td
(LOD)Tj
2.2393 0 Td
(=)Tj
0.7994 0 Td
(mean)Tj
2.4434 0 Td
(concentration)Tj
5.8224 0 Td
(response)Tj
3.7303 0 Td
(+)Tj
0.7994 0 Td
(\(3)Tj
1.0375 0 Td
(x)Tj
0.6859 0 Td
(Std.)Tj
1.7462 0 Td
(Dev.\))Tj
-24.1454 -1.8992 Td
(6.)Tj
1.2019 0 Td
(Limits)Tj
2.7666 0 Td
(of)Tj
1.0148 0 Td
(Quantitation:)Tj
5.6239 0 Td
(The)Tj
1.7801 0 Td
(lower)Tj
2.4548 0 Td
(limit)Tj
2.1203 0 Td
(of)Tj
1.0148 0 Td
(quantitation)Tj
5.1534 0 Td
(\(LLOQ\))Tj
3.4696 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.474 0 Td
(upper)Tj
2.5737 0 Td
(limit)Tj
2.1203 0 Td
(of)Tj
-33.2954 -1.2982 Td
(quantitation)Tj
5.1477 0 Td
(\(ULOQ\))Tj
3.6567 0 Td
(were)Tj
2.1146 0 Td
(established)Tj
4.5864 0 Td
(for)Tj
1.3776 0 Td
(the)Tj
1.4627 0 Td
(method)Tj
3.3165 0 Td
(and)Tj
1.7235 0 Td
(defined)Tj
3.2144 0 Td
(as)Tj
1.0092 0 Td
(the)Tj
1.457 0 Td
(lowest)Tj
2.7325 0 Td
(and)Tj
1.7234 0 Td
(highest)Tj
-33.5222 -1.304 Td
(validated)Tj
3.8324 0 Td
(concentration)Tj
5.8167 0 Td
(that)Tj
1.7801 0 Td
(met)Tj
1.7632 0 Td
(all)Tj
1.1451 0 Td
(acceptance)Tj
4.5695 0 Td
(criteria.)Tj
3.2995 0 Td
(Accuracy)Tj
3.9628 0 Td
(and)Tj
1.7291 0 Td
(Precision)Tj
3.9514 0 Td
(was)Tj
1.6951 0 Td
(estab-)Tj
-33.5449 -1.2982 Td
(lished)Tj
2.5738 0 Td
(at)Tj
0.9468 0 Td
(the)Tj
1.474 0 Td
(LLOQ.)Tj
-6.1965 -1.8992 Td
(7.)Tj
1.2019 0 Td
(Stability)Tj
3.4639 0 Td
(of)Tj
1.0148 0 Td
(Processed)Tj
4.1783 0 Td
(Samples:)Tj
-8.657 -1.304 Td
(4C:)Tj
1.9672 0 Td
(re-injection)Tj
4.9039 0 Td
(stability)Tj
3.3449 0 Td
(was)Tj
1.6951 0 Td
(assessed)Tj
3.4639 0 Td
(by)Tj
1.1792 0 Td
(repeat)Tj
2.6816 0 Td
(injection)Tj
3.753 0 Td
(of)Tj
1.0092 0 Td
(a)Tj
0.6463 0 Td
(complete)Tj
3.8777 0 Td
(set)Tj
1.2926 0 Td
(of)Tj
1.009 0 Td
(aged)Tj
2.0637 0 Td
(standard)Tj
-32.8873 -1.2982 Td
(samples)Tj
3.3789 0 Td
(after)Tj
2.0352 0 Td
(storage)Tj
3.0841 0 Td
(at)Tj
0.9525 0 Td
(10C)Tj
2.2223 0 Td
(for)Tj
1.389 0 Td
(5)Tj
0.6973 0 Td
(days.)Tj
2.2223 0 Td
(The)Tj
1.7802 0 Td
(re-injection)Tj
4.9039 0 Td
(run)Tj
1.6667 0 Td
(was)Tj
1.7008 0 Td
(considered)Tj
4.6034 0 Td
(successful)Tj
4.1612 0 Td
(if)Tj
-34.7978 -1.2983 Td
(the)Tj
1.4683 0 Td
(mean)Tj
2.4435 0 Td
(concentration)Tj
5.8223 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(stability)Tj
3.3506 0 Td
(samples)Tj
3.3845 0 Td
(was)Tj
1.7008 0 Td
(within)Tj
/F12 1 Tf
2.812 0 Td
[()]TJ
/F7 1 Tf
(15%)Tj
2.5001 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(mean)Tj
2.4434 0 Td
(of)Tj
1.0148 0 Td
(freshly-)Tj
-31.9065 -1.2982 Td
(prepared)Tj
3.8041 0 Td
(samples)Tj
3.3845 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(same)Tj
2.2563 0 Td
(theoretical)Tj
4.439 0 Td
(concentration.)Tj
-16.3671 -1.304 Td
(-20C)Tj
2.5738 0 Td
(and)Tj
1.7292 0 Td
(freeze/thaw:)Tj
5.0626 0 Td
(The)Tj
1.7858 0 Td
(stability)Tj
3.3506 0 Td
(was)Tj
1.7007 0 Td
(considered)Tj
4.6035 0 Td
(acceptable)Tj
4.3483 0 Td
(if)Tj
0.7767 0 Td
(the)Tj
1.4683 0 Td
(mean)Tj
2.4435 0 Td
(of)Tj
1.0147 0 Td
(the)Tj
1.4683 0 Td
(freeze/)Tj
-32.326 -1.2982 Td
(thaw)Tj
2.16 0 Td
(samples)Tj
3.3845 0 Td
(\(stored)Tj
3.0501 0 Td
(at)Tj
0.9524 0 Td
(-20C\))Tj
2.914 0 Td
(was)Tj
1.7008 0 Td
(within)Tj
/F12 1 Tf
2.812 0 Td
[()]TJ
/F7 1 Tf
(15%)Tj
2.5001 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(mean)Tj
2.4435 0 Td
(of)Tj
1.0148 0 Td
(freshly-)Tj
3.2428 0 Td
(prepared)Tj
3.8096 0 Td
(samples.)Tj
-32.4677 -1.2983 Td
(Calibration)Tj
4.7622 0 Td
(standards)Tj
4.0818 0 Td
(were)Tj
2.126 0 Td
(evaluated)Tj
3.9855 0 Td
(over)Tj
1.9786 0 Td
(2)Tj
0.6973 0 Td
(months,)Tj
3.5036 0 Td
(at)Tj
0.9524 0 Td
(day)Tj
1.6384 0 Td
(34)Tj
1.1736 0 Td
(and)Tj
1.7291 0 Td
(62)Tj
1.1679 0 Td
(after)Tj
2.0352 0 Td
(preparation.)Tj
-29.8316 -1.3039 Td
(The)Tj
1.7801 0 Td
(samples)Tj
3.3846 0 Td
(went)Tj
2.1713 0 Td
(through)Tj
3.4469 0 Td
(6)Tj
0.6973 0 Td
(freeze-thaw)Tj
4.8529 0 Td
(cycles)Tj
2.5569 0 Td
(between)Tj
3.5262 0 Td
(day)Tj
1.6385 0 Td
(1)Tj
0.6916 0 Td
(and)Tj
1.7348 0 Td
(34)Tj
1.1679 0 Td
(and)Tj
1.7291 0 Td
(3)Tj
0.6973 0 Td
(freeze-thaw)Tj
-30.0754 -1.2983 Td
(cycles)Tj
2.5512 0 Td
(between)Tj
3.5262 0 Td
(day)Tj
1.6385 0 Td
(34)Tj
1.1678 0 Td
(and)Tj
1.7348 0 Td
(day)Tj
1.6328 0 Td
(62.)Tj
1.4003 0 Td
(Values)Tj
2.9026 0 Td
(obtained)Tj
3.7361 0 Td
(were)Tj
2.1203 0 Td
(further)Tj
3.0274 0 Td
(evaluated)Tj
3.9911 0 Td
(by)Tj
1.1848 0 Td
(ANOVA)Tj
3.7644 0 Td
(to)Tj
-34.3783 -1.2982 Td
(detect)Tj
2.6135 0 Td
(any)Tj
1.6555 0 Td
(significant)Tj
4.388 0 Td
(difference)Tj
4.2065 0 Td
(between)Tj
3.5263 0 Td
(measurements.)Tj
-17.5917 -1.8992 Td
(8.)Tj
1.2019 0 Td
(Analysis)Tj
3.5773 0 Td
(of)Tj
1.0148 0 Td
(Other)Tj
2.5852 0 Td
(Plant)Tj
2.3074 0 Td
(Samples:)Tj
-9.4847 -1.304 Td
(Several)Tj
3.0444 0 Td
(plant)Tj
2.2677 0 Td
(samples)Tj
3.3789 0 Td
(were)Tj
2.1259 0 Td
(selected)Tj
3.3449 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.474 0 Td
(basis)Tj
2.1486 0 Td
(of)Tj
1.0148 0 Td
(their)Tj
2.109 0 Td
(common)Tj
3.8438 0 Td
(occurrence)Tj
4.66 0 Td
(in)Tj
1.0375 0 Td
(or)Tj
1.0998 0 Td
(near)Tj
1.9956 0 Td
(UK)Tj
-34.8205 -1.2982 Td
(pastures)Tj
3.5093 0 Td
(and)Tj
1.7348 0 Td
(similarity)Tj
4.0025 0 Td
(or)Tj
1.0998 0 Td
(possible)Tj
3.4129 0 Td
(confusion)Tj
4.1952 0 Td
(with)Tj
1.9956 0 Td
(sycamores,)Tj
4.6034 0 Td
(their)Tj
2.109 0 Td
(species,)Tj
3.2201 0 Td
(or)Tj
1.0998 0 Td
(their)Tj
2.1089 0 Td
(known)Tj
-33.0913 -1.2983 Td
(toxicity)Tj
3.1975 0 Td
(in)Tj
1.0374 0 Td
(horses.)Tj
2.9991 0 Td
(They)Tj
2.2337 0 Td
(included)Tj
3.7133 0 Td
(sycamore)Tj
4.0139 0 Td
(and)Tj
1.7291 0 Td
(other)Tj
/F8 1 Tf
2.347 0 Td
[(\r)30()7()2()]TJ
/F7 1 Tf
2.0013 0 Td
(trees)Tj
2.0976 0 Td
(\()Tj
/F8 1 Tf
[(\r)]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.4343 0 Td
[()7()-3()2()4()4()11()10()4()22()11()16()18()-4()]TJ
/F7 1 Tf
(,)Tj
/F8 1 Tf
6.5026 0 Td
[(\r)]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.0998 0 Td
[()7()16()]TJ
-34.4066 -1.3039 Td
[()4()8()9()7()20()]TJ
/F7 1 Tf
(,)Tj
/F8 1 Tf
2.6872 0 Td
[(\r)]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
[()10()4()22()11()16()6()11()4()4()8()]TJ
/F7 1 Tf
(\))Tj
5.8904 0 Td
(horse)Tj
2.4094 0 Td
(chestnut)Tj
3.6227 0 Td
(\()Tj
/F8 1 Tf
[(\r)]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.4286 0 Td
[()18()10()11()4()4()7()9()11()16()18()12()]TJ
/F7 1 Tf
(,)Tj
6.7578 0 Td
(ash)Tj
1.5363 0 Td
(\()Tj
/F8 1 Tf
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
[()1()-4()7()15()-4()10()4()11()]TJ
/F7 1 Tf
(\),)Tj
5.1421 0 Td
(ragwort)Tj
3.3447 0 Td
(\()Tj
/F8 1 Tf
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.0885 0 Td
[()7()3()15(\n)3()8()]TJ
-33.9077 -1.2983 Td
[()7()-9()-4()]TJ
/F7 1 Tf
(\),)Tj
2.0409 0 Td
(common)Tj
3.8438 0 Td
(mallow)Tj
3.1578 0 Td
(\()Tj
/F8 1 Tf
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.6554 0 Td
[()7()3()15(\n)3()8()9()7()-9()-4()]TJ
/F7 1 Tf
(,)Tj
4.1782 0 Td
(oak)Tj
1.6555 0 Td
(\()Tj
/F8 1 Tf
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.491 0 Td
[()20()13()11()-4()]TJ
/F7 1 Tf
(\),)Tj
2.9423 0 Td
(yew)Tj
1.7858 0 Td
(\()Tj
/F8 1 Tf
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.372 0 Td
[()4()6()7()7()22()11()]TJ
/F7 1 Tf
3.5149 0 Td
(and)Tj
1.7348 0 Td
(beech)Tj
2.5227 0 Td
(\()Tj
/F8 1 Tf
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
-31.8951 -1.2982 Td
[()7()3()15(\n)3()22()7()4()7()]TJ
/F7 1 Tf
(\).)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 357.6755 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Detection)Tj
4.3724 0 Td
(of)Tj
1.0418 0 Td
(hypoglyc)Tj
3.8693 0 Td
(in)Tj
0.985 0 Td
(A)Tj
0.8788 0 Td
(in)Tj
0.9779 0 Td
(plant)Tj
2.3457 0 Td
(material)Tj
3.7064 0 Td
(without)Tj
3.3307 0 Td
(derivatisa)Tj
4.1953 0 Td
(tion)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01995)Tj
4.089 0 Td
(21)Tj
0 g
2.0906 0 Td
(July)Tj
1.9701 0 Td
(3,)Tj
1.0417 0 Td
(2018)Tj
35.894 0 Td
(4)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(11)Tj
ET
Q
endstream
endobj
82 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 15 0 R/Annots 84 0 R/Contents 85 0 R/TrimBox[0 0 612 792]>>
endobj
83 0 obj
<>stream
x!TL.#_D"-H$%%%D"H$rK$?:]֜t2i;i-/$g-Vv.Ꜯ{{t] @ @ @ @`???ϟ?(Y߿_^^u~~~ssN<==]__G1Cu>>>v]\r JNNNuٿ, @ @ @ @ 4'MD(%>+sGia_d~^^^vHe\>;;.'G$RB)[s$" .ϣc @ @ @ @ f'Pv'%Ojc^<+'M0 MJLDL4w @ @ @ @ -Sbٗ[D&n-,m 4mtI+uA#|Pp{{^7: @ @ @ @`F̮l/T;&fݙ<úfoZSalC&! @ @ @ @`E9Eeڴ6r>:MLY)s0w!@ @ @ @ +rwQ-|Ù*ϢΩ>[,\ @ @ @ @?$|m&woiv3u}}n6AlA]8??h @ @ @ @ c1Qu@&'4B8#.Aa&BMt1
&%@ @ @ @ XW MzLy||\qGf]n&C.`~~mbu˳= @ @ @ @8 >999hEnY)3Ƴ @ @ @ @@@Qc&[e=f%XшhbGaQޢ(ׯ_ke~nnnfy%]ߺ[ @ @ @ @ \|Io㩢>QI,k|}}]]]v쭊@r'喻^'QLaC([8˝Z"Ub^RURx^^ @ @ @ @ L%&