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Hirofumi Morihara, Masanori Obana, Shota Tanaka, Ikki Kawakatsu, Daisuke Tsuchiyama, Shota Mori, Hiroshi Suizu, Akiko Ishida, Rumi Kimura, Izuru Tsuchimochi, Makiko Maeda, Takehiko Yoshimitsu, Yasushi Fujio, Hiroyuki Nakayama
2-aminoethoxydiphenyl borate provides an anti-oxidative effect and mediates cardioprotection during ischemia reperfusion in mice
www.plosone.org
www.plosone.org
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2.3583 0 Td
(xestospongin)Tj
5.4935 0 Td
(C)Tj
0.8788 0 Td
(\(Wako)Tj
2.965 0 Td
(Pure)Tj
2.1033 0 Td
(Chemical)Tj
4.0252 0 Td
(Indus-)Tj
-34.1459 -1.3039 Td
(tries,)Tj
2.1657 0 Td
(Japan\),)Tj
3.0501 0 Td
(SKF-96365)Tj
4.6261 0 Td
(\(Focus)Tj
2.9026 0 Td
(Biomolecules,)Tj
5.7769 0 Td
(Plymouth)Tj
4.1612 0 Td
(Meeting,)Tj
3.7644 0 Td
(PA\),)Tj
2.0296 0 Td
(mefenamic)Tj
4.6658 0 Td
(acid)Tj
-33.1424 -1.2983 Td
(\(Tokyo)Tj
3.1351 0 Td
(Chemical)Tj
4.0252 0 Td
(Industry)Tj
3.651 0 Td
(Co,)Tj
1.6157 0 Td
(Japan\),)Tj
3.0444 0 Td
(2-APB,)Tj
3.0953 0 Td
(AA-861)Tj
3.3676 0 Td
(and)Tj
1.7291 0 Td
(phenylephrine)Tj
6.0208 0 Td
(\(Sigma-Aldrich,)Tj
-29.6842 -1.2983 Td
(St.)Tj
1.2189 0 Td
(Louis,)Tj
2.6476 0 Td
(MO\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 516.1889 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Neonatal)Tj
4.1669 0 Td
(rat)Tj
1.3748 0 Td
(cardiomyocyte)Tj
6.6001 0 Td
(\(NRCM\))Tj
3.8551 0 Td
(isolation)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 499.1811 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Neonatal)Tj
3.8437 0 Td
(rats)Tj
1.6894 0 Td
(were)Tj
2.1203 0 Td
(purchased)Tj
4.3314 0 Td
(from)Tj
2.211 0 Td
(Kiwa)Tj
2.279 0 Td
(Laboratory)Tj
4.6375 0 Td
(Animals)Tj
3.5829 0 Td
(Co.Ltd.)Tj
3.2032 0 Td
(NRCMs)Tj
3.4979 0 Td
(were)Tj
2.1203 0 Td
(isolated)Tj
-33.5166 -1.2982 Td
(and)Tj
1.7291 0 Td
(cultured)Tj
3.5659 0 Td
(as)Tj
1.0148 0 Td
(previously)Tj
4.3484 0 Td
(described)Tj
4.0535 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(].)Tj
2.0806 0 Td
(In)Tj
1.1055 0 Td
(brief,)Tj
2.3017 0 Td
(hearts)Tj
2.6362 0 Td
(excised)Tj
3.1124 0 Td
(from)Tj
2.2054 0 Td
(neonatal)Tj
3.6623 0 Td
(rats)Tj
1.6838 0 Td
(were)Tj
-33.4996 -1.2983 Td
(minced)Tj
3.2314 0 Td
(and)Tj
1.7291 0 Td
(digested)Tj
3.515 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
0.6519 0 Td
(solution)Tj
3.4866 0 Td
(containing)Tj
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(0.1%)Tj
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(collagenase)Tj
4.7338 0 Td
(type)Tj
1.9106 0 Td
(IV)Tj
1.2585 0 Td
(\(Sigma-Aldrich,)Tj
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(St.)Tj
1.2188 0 Td
(Louis,)Tj
2.6476 0 Td
(MO\))Tj
2.1997 0 Td
(and)Tj
1.7291 0 Td
(0.1%)Tj
2.1543 0 Td
(trypsin)Tj
3.0387 0 Td
(\(Thermo)Tj
3.8211 0 Td
(Fisher)Tj
2.6929 0 Td
(Scientific,)Tj
4.1329 0 Td
(Waltham,)Tj
4.1669 0 Td
(MA\))Tj
2.1373 0 Td
(to)Tj
1.0261 0 Td
(obtain)Tj
2.7836 0 Td
(a)Tj
0.652 0 Td
(suspen-)Tj
-34.401 -1.2983 Td
(sion)Tj
1.9048 0 Td
(of)Tj
1.0148 0 Td
(single)Tj
2.5285 0 Td
(cells.)Tj
2.1487 0 Td
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2.2847 0 Td
(incubating)Tj
4.507 0 Td
(the)Tj
1.4684 0 Td
(cells)Tj
1.9218 0 Td
(in)Tj
1.0318 0 Td
(a)Tj
0.6577 0 Td
(culture)Tj
3.0387 0 Td
(dish)Tj
1.8992 0 Td
(for)Tj
1.389 0 Td
(6090)Tj
2.6418 0 Td
(min)Tj
1.8539 0 Td
(at)Tj
0.9524 0 Td
(37C)Tj
2.228 0 Td
(in)Tj
1.0318 0 Td
(5%)Tj
-34.503 -1.2982 Td
(CO)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 214.0724 432.5102 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 219.8551 434.211 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(atmosphere,)Tj
5.1306 0 Td
(floating)Tj
3.2712 0 Td
(cells)Tj
1.9219 0 Td
(were)Tj
2.1203 0 Td
(collected)Tj
3.7417 0 Td
(as)Tj
1.0148 0 Td
(NRCMs.)Tj
3.719 0 Td
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3.3562 0 Td
(NRCMs)Tj
3.4979 0 Td
(were)Tj
2.1203 0 Td
(cultured)Tj
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(in)Tj
-35.4441 -1.2982 Td
(Dulbecco's)Tj
4.5863 0 Td
(Modified)Tj
3.9232 0 Td
(Eagles)Tj
2.7212 0 Td
(Medium)Tj
3.6794 0 Td
(\(DMEM;)Tj
3.8721 0 Td
(Sigma-Aldrich,)Tj
6.3042 0 Td
(St.)Tj
1.2245 0 Td
(Louis,)Tj
2.6419 0 Td
(MO\))Tj
2.1997 0 Td
(containing)Tj
4.5297 0 Td
(high)Tj
-35.6822 -1.3039 Td
(glucose)Tj
3.1747 0 Td
(\(4.5)Tj
1.7348 0 Td
(g/L\))Tj
1.8935 0 Td
(with)Tj
1.9956 0 Td
(L-glutamine)Tj
5.142 0 Td
(\(4)Tj
1.0318 0 Td
(mM\))Tj
2.2734 0 Td
(and)Tj
1.7291 0 Td
(sodium)Tj
3.2315 0 Td
(bicarbonate)Tj
4.9436 0 Td
(\(3.7)Tj
1.7348 0 Td
(g/L\),)Tj
2.1203 0 Td
(supplemented)Tj
-31.0051 -1.2983 Td
(with)Tj
1.9955 0 Td
(10%)Tj
1.9275 0 Td
(fetal)Tj
1.9106 0 Td
(bovine)Tj
2.931 0 Td
(serum)Tj
2.7212 0 Td
(\(FBS\))Tj
2.4832 0 Td
(and)Tj
1.7291 0 Td
(bromodeoxyuridine)Tj
8.2431 0 Td
(\(0.1)Tj
/F12 1 Tf
1.7348 0 Td
[()]TJ
/F8 1 Tf
(g/mL;)Tj
3.1067 0 Td
(Sigma-Aldrich,)Tj
-28.7827 -1.2983 Td
(St.)Tj
1.2189 0 Td
(Louis,)Tj
2.6474 0 Td
(MO\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 353.5937 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Cell)Tj
1.9134 0 Td
(viability)Tj
3.4488 0 Td
(assays)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 336.5858 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(isolated)Tj
3.2882 0 Td
(NRCMs)Tj
3.4922 0 Td
(were)Tj
2.1203 0 Td
(cultured)Tj
3.566 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
0.6519 0 Td
(serum-free)Tj
4.5808 0 Td
(medium)Tj
3.6056 0 Td
(for)Tj
1.389 0 Td
(24)Tj
1.1679 0 Td
(h)Tj
0.7483 0 Td
(and)Tj
1.7348 0 Td
(were)Tj
2.1203 0 Td
(pretreated)Tj
4.3086 0 Td
(with)Tj
-35.5915 -1.2982 Td
(2-APB,)Tj
3.0954 0 Td
(specific)Tj
3.1861 0 Td
(1,4,5-Inositol)Tj
5.5275 0 Td
(trisphosphate)Tj
5.6296 0 Td
(receptor)Tj
3.5546 0 Td
(blocker)Tj
3.1975 0 Td
(\(xestospongin)Tj
5.828 0 Td
(C\),)Tj
1.4513 0 Td
(TRP)Tj
2.0069 0 Td
(channel)Tj
-33.4769 -1.2983 Td
(blocker)Tj
3.1917 0 Td
(\(SKF-96365\),)Tj
5.5332 0 Td
(TRPM7)Tj
3.3789 0 Td
(blocker)Tj
3.1975 0 Td
(\(AA-861\))Tj
4.0422 0 Td
(or)Tj
1.0998 0 Td
(TRPM3)Tj
3.3789 0 Td
(blocker)Tj
3.1974 0 Td
(\(mefenamic)Tj
5.006 0 Td
(acid\),)Tj
2.4378 0 Td
(1)Tj
0.6973 0 Td
(h)Tj
-35.1607 -1.3039 Td
(before)Tj
2.7382 0 Td
(the)Tj
1.4683 0 Td
(addition)Tj
3.6 0 Td
(of)Tj
1.0148 0 Td
(H)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 295.8803 295.937 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 299.4519 297.5811 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 306.9354 295.937 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 310.4503 297.5811 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(.)Tj
0.4478 0 Td
(An)Tj
1.4514 0 Td
(equal)Tj
2.3584 0 Td
(volume)Tj
3.2031 0 Td
(of)Tj
1.0148 0 Td
(vehicles)Tj
3.3448 0 Td
(\(i.e.,)Tj
1.9276 0 Td
(ethanol)Tj
3.2031 0 Td
(for)Tj
1.389 0 Td
(xestospongin)Tj
5.4878 0 Td
(C)Tj
0.8844 0 Td
(and)Tj
-35.7559 -1.2982 Td
(DMSO)Tj
3.0728 0 Td
(for)Tj
1.3833 0 Td
(SKF-96365,)Tj
4.8529 0 Td
(AA-861,)Tj
3.5885 0 Td
(mefenamic)Tj
4.6658 0 Td
(acid\))Tj
2.2167 0 Td
(was)Tj
1.6951 0 Td
(added)Tj
2.6645 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.652 0 Td
(control)Tj
3.1238 0 Td
(1)Tj
0.6916 0 Td
(h)Tj
0.7483 0 Td
(before)Tj
2.744 0 Td
(the)Tj
1.4683 0 Td
(addi-)Tj
-34.5824 -1.2983 Td
(tion)Tj
1.8425 0 Td
(of)Tj
1.0148 0 Td
(H)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 236.2393 269.9149 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 239.811 271.6157 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 247.2377 269.9149 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 250.8094 271.6157 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(.)Tj
0.4478 0 Td
(Four)Tj
2.1543 0 Td
(hours)Tj
2.5115 0 Td
(after)Tj
2.0353 0 Td
(the)Tj
1.4683 0 Td
(addition)Tj
3.6 0 Td
(of)Tj
1.0148 0 Td
(H)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 390.7842 269.9149 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 394.3559 271.6157 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 401.8393 269.9149 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 405.3543 271.6157 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(,)Tj
0.4478 0 Td
(cell)Tj
1.5591 0 Td
(viability)Tj
3.4185 0 Td
(was)Tj
1.6951 0 Td
(measured)Tj
4.1102 0 Td
(using)Tj
2.3925 0 Td
(the)Tj
1.4683 0 Td
(Cell-)Tj
-35.6255 -1.3039 Td
(Titer)Tj
2.1883 0 Td
(Blue)Tj
2.0013 0 Td
(assay)Tj
2.262 0 Td
(kit)Tj
1.2813 0 Td
(\(Promega,)Tj
4.3653 0 Td
(Madison,)Tj
3.9855 0 Td
(WI\),)Tj
2.0919 0 Td
(according)Tj
4.1839 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(manufacturer's)Tj
6.2646 0 Td
(protocol.)Tj
3.8267 0 Td
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-34.9452 -1.2982 Td
(assay)Tj
2.262 0 Td
(is)Tj
0.8504 0 Td
(based)Tj
2.4718 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(ability)Tj
2.6929 0 Td
(of)Tj
1.0148 0 Td
(living)Tj
2.4718 0 Td
(cells)Tj
1.9219 0 Td
(to)Tj
1.0261 0 Td
(convert)Tj
3.2428 0 Td
(a)Tj
0.6576 0 Td
(redox)Tj
2.5115 0 Td
(dye)Tj
1.627 0 Td
(\(resazurin\))Tj
4.6205 0 Td
(into)Tj
1.8368 0 Td
(a)Tj
0.6577 0 Td
(fluorescent)Tj
-32.6095 -1.2983 Td
(product)Tj
3.3902 0 Td
(\(resorufin\).)Tj
4.7905 0 Td
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1.7859 0 Td
(cells)Tj
1.9162 0 Td
(in)Tj
1.0374 0 Td
(96-well)Tj
3.1238 0 Td
(culture)Tj
3.0387 0 Td
(plate)Tj
2.1372 0 Td
(were)Tj
2.126 0 Td
(incubated)Tj
4.1726 0 Td
(with)Tj
2.0012 0 Td
(20)Tj
/F12 1 Tf
1.1679 0 Td
[()]TJ
/F8 1 Tf
(L)Tj
1.2642 0 Td
(of)Tj
1.0148 0 Td
(CellTiter)Tj
-32.9666 -1.2983 Td
(Blue)Tj
1.9956 0 Td
(reagent)Tj
3.1861 0 Td
(for)Tj
1.3833 0 Td
(3)Tj
0.6973 0 Td
(h)Tj
0.7484 0 Td
(at)Tj
0.9524 0 Td
(37C)Tj
2.228 0 Td
(in)Tj
1.0318 0 Td
(a)Tj
0.6577 0 Td
(humidified,)Tj
4.8869 0 Td
(5%)Tj
1.4513 0 Td
(CO)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 406.3181 217.9275 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 412.1007 219.6283 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(atmosphere.)Tj
5.125 0 Td
(The)Tj
1.7858 0 Td
(fluorescence)Tj
5.1873 0 Td
(was)Tj
1.6951 0 Td
(mea-)Tj
-35.0018 -1.3039 Td
(sured)Tj
2.4321 0 Td
(using)Tj
2.3867 0 Td
(a)Tj
0.652 0 Td
(96-well)Tj
3.1238 0 Td
(plate)Tj
2.1373 0 Td
(reader)Tj
2.7722 0 Td
(\(SpectraMAX)Tj
5.7373 0 Td
(M5e,)Tj
2.2336 0 Td
(excitation)Tj
4.1556 0 Td
(at)Tj
0.9524 0 Td
(560)Tj
1.6441 0 Td
(nm)Tj
1.5874 0 Td
(and)Tj
1.7348 0 Td
(emission)Tj
3.77 0 Td
(at)Tj
-35.3193 -1.2982 Td
(590)Tj
1.644 0 Td
(nm;)Tj
1.8142 0 Td
(Molecular)Tj
4.2916 0 Td
(Devices,)Tj
3.5377 0 Td
(Sunnyvale,)Tj
4.558 0 Td
(CA\))Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 164.9763 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Intracellular)Tj
5.315 0 Td
(calcium)Tj
3.5716 0 Td
(measurements)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 148.0251 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(To)Tj
1.3322 0 Td
(elucidate)Tj
3.7871 0 Td
(the)Tj
1.4683 0 Td
(underlying)Tj
4.5921 0 Td
(mechanism)Tj
4.8416 0 Td
(of)Tj
1.0091 0 Td
(Ca)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 381.3165 152.4472 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 391.3511 148.0251 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(chelator)Tj
3.4469 0 Td
(mediated)Tj
3.9401 0 Td
(inhibition)Tj
4.2179 0 Td
(in)Tj
1.0318 0 Td
(H)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 525.4299 146.3244 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 529.0015 148.0251 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 536.4283 146.3244 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 540 148.0251 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(-induced)Tj
-33.9985 -1.3039 Td
(cardiomyocyte)Tj
6.1285 0 Td
(death,)Tj
2.6589 0 Td
(intracellular)Tj
5.0569 0 Td
(calcium)Tj
3.3619 0 Td
(levels)Tj
2.3754 0 Td
(were)Tj
2.126 0 Td
(monitored)Tj
4.4901 0 Td
(as)Tj
1.0148 0 Td
(previously)Tj
4.3483 0 Td
(reported)Tj
3.6622 0 Td
(with)Tj
-35.223 -1.2982 Td
(modifications)Tj
5.7373 0 Td
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-0.004 Tc
0 0 Td
(To)Tj
1.3379 0 Td
(examine)Tj
3.6 0 Td
(the)Tj
1.4683 0 Td
(direct)Tj
2.5342 0 Td
(interaction)Tj
4.6091 0 Td
(between)Tj
3.5262 0 Td
(2-APB)Tj
2.8687 0 Td
(and)Tj
1.7291 0 Td
(H)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 424.4598 436.8188 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 427.9748 438.4629 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 435.4582 436.8188 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 439.0299 438.4629 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(,)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 443.4519 442.885 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 447.0236 438.4629 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(H-NMR)Tj
3.5886 0 Td
(measurement)Tj
5.6692 0 Td
(and)Tj
-33.9587 -1.2982 Td
(thin-layer)Tj
4.1386 0 Td
(chromatography)Tj
6.8824 0 Td
(were)Tj
2.1203 0 Td
(performed)Tj
4.4674 0 Td
([)Tj
0.83 0.64 0.02 0 k
(28)Tj
0 g
(])Tj
1.8539 0 Td
([)Tj
0.83 0.64 0.02 0 k
(29)Tj
0 g
(].)Tj
2.0749 0 Td
(Five)Tj
1.8935 0 Td
(milligrams)Tj
4.5183 0 Td
(of)Tj
1.0148 0 Td
(2-APB)Tj
2.8687 0 Td
(\(0.0222)Tj
-31.8328 -1.3039 Td
(mmol\))Tj
2.9537 0 Td
(were)Tj
2.1203 0 Td
(dissolved)Tj
3.8948 0 Td
(in)Tj
1.0374 0 Td
(methanol-)Tj
/F14 1 Tf
[()]TJ
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 346.5637 410.7968 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(4)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 352.3464 412.4409 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\(0.6)Tj
1.7347 0 Td
(mL\))Tj
1.9219 0 Td
(in)Tj
1.0318 0 Td
(an)Tj
1.2019 0 Td
(NMR)Tj
2.4718 0 Td
(tube)Tj
1.9786 0 Td
(at)Tj
0.9524 0 Td
(room)Tj
2.4208 0 Td
(temperature,)Tj
5.3574 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
-36.0336 -1.2982 Td
(mixture)Tj
3.3902 0 Td
(was)Tj
1.7008 0 Td
(subjected)Tj
3.9684 0 Td
(to)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 300.8692 403.937 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 304.4409 399.4582 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(H-NMR)Tj
3.5886 0 Td
(analysis)Tj
3.3222 0 Td
(to)Tj
1.0261 0 Td
(obtain)Tj
2.7836 0 Td
(an)Tj
1.2019 0 Td
(authentic)Tj
3.9685 0 Td
(spectrum)Tj
3.9628 0 Td
(\(spectrum)Tj
4.3029 0 Td
(A\).)Tj
-34.5993 -1.2982 Td
(H)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 207.6661 384.7748 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 211.2377 386.4755 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 218.7212 384.7748 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 224.4472 386.4755 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\(31%)Tj
2.2677 0 Td
(w/v,)Tj
1.9162 0 Td
(25)Tj
/F12 1 Tf
1.1678 0 Td
[()]TJ
/F8 1 Tf
(L,)Tj
1.491 0 Td
(0.222)Tj
2.3528 0 Td
(mmol\))Tj
2.948 0 Td
(was)Tj
1.7008 0 Td
(then)Tj
2.0182 0 Td
(added)Tj
2.6589 0 Td
(to)Tj
1.0261 0 Td
(this)Tj
1.6725 0 Td
(mixture.)Tj
3.6226 0 Td
(After)Tj
2.2847 0 Td
(shaking)Tj
3.3166 0 Td
(for)Tj
1.3889 0 Td
(a)Tj
0.652 0 Td
(few)Tj
-34.9282 -1.2982 Td
(seconds,)Tj
3.5942 0 Td
(the)Tj
1.474 0 Td
(mixture)Tj
3.3902 0 Td
(was)Tj
1.7008 0 Td
(subjected)Tj
3.9685 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 366.2362 377.9149 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 369.8078 373.4929 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(H-NMR)Tj
3.5886 0 Td
(analysis)Tj
3.3222 0 Td
(and)Tj
1.7348 0 Td
(the)Tj
1.4683 0 Td
(second)Tj
3.0104 0 Td
(spectrum)Tj
3.9628 0 Td
(was)Tj
-34.0665 -1.3039 Td
(recorded)Tj
3.7928 0 Td
(in)Tj
1.0374 0 Td
(5)Tj
0.6974 0 Td
(min)Tj
1.8538 0 Td
(\(spectrum)Tj
4.303 0 Td
(B\).)Tj
1.3663 0 Td
(After)Tj
2.2847 0 Td
(60)Tj
1.1678 0 Td
(min,)Tj
2.0805 0 Td
(the)Tj
1.4741 0 Td
(mixture)Tj
3.3902 0 Td
(was)Tj
1.7007 0 Td
(analyzed)Tj
3.7021 0 Td
(to)Tj
1.0261 0 Td
(obtain)Tj
2.7779 0 Td
(the)Tj
1.4684 0 Td
(third)Tj
-34.1232 -1.2982 Td
(spectrum)Tj
3.9628 0 Td
(\(spectrum)Tj
4.303 0 Td
(C\).)Tj
1.4457 0 Td
(All)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 311.1307 351.8929 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 314.6456 347.4708 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(H-NMR)Tj
3.5886 0 Td
(spectra)Tj
3.05 0 Td
(were)Tj
2.1203 0 Td
(recorded)Tj
3.7985 0 Td
(on)Tj
1.2755 0 Td
(a)Tj
0.652 0 Td
(300)Tj
1.6498 0 Td
(MHz)Tj
2.2903 0 Td
(JEOL)Tj
2.4038 0 Td
(AL-300)Tj
3.2201 0 Td
(spec-)Tj
-35.5121 -1.2982 Td
(trometer)Tj
3.7304 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(deuterated)Tj
4.4787 0 Td
(solvent,)Tj
3.2824 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4684 0 Td
(chemical)Tj
3.787 0 Td
(shifts)Tj
2.3244 0 Td
(were)Tj
2.1203 0 Td
(reported)Tj
3.6624 0 Td
(with)Tj
1.9955 0 Td
(a)Tj
0.6577 0 Td
(residual)Tj
3.3732 0 Td
(peak)Tj
-35.1153 -1.3039 Td
(of)Tj
1.0148 0 Td
(CD)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 224.1637 319.8047 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 227.6787 321.4488 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(HOD)Tj
2.4717 0 Td
(as)Tj
1.0148 0 Td
(an)Tj
1.2019 0 Td
(internal)Tj
3.3619 0 Td
(standard.)Tj
3.9458 0 Td
(The)Tj
1.7801 0 Td
(reaction)Tj
3.498 0 Td
(products)Tj
3.753 0 Td
(were)Tj
2.1203 0 Td
(further)Tj
3.0274 0 Td
(examined)Tj
4.1329 0 Td
(by)Tj
1.1792 0 Td
(thin-)Tj
-34.2535 -1.2982 Td
(layer)Tj
2.1486 0 Td
(chromatography)Tj
6.8767 0 Td
(\(TLC\))Tj
2.7156 0 Td
(using)Tj
2.3867 0 Td
(TLC)Tj
2.0353 0 Td
(plates)Tj
2.5001 0 Td
(\(TLC)Tj
2.3698 0 Td
(silica)Tj
2.2223 0 Td
(gel)Tj
1.355 0 Td
(60)Tj
1.1679 0 Td
(F)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 463.0677 306.822 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(254)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 473.7826 308.4661 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(;)Tj
0.4422 0 Td
(Merck)Tj
2.8289 0 Td
(Millipore,)Tj
4.1839 0 Td
(Biller-)Tj
-34.8318 -1.2982 Td
(ica,)Tj
1.5704 0 Td
(MA\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 269.0078 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(I/R)Tj
1.4882 0 Td
(model)Tj
2.9102 0 Td
(and)Tj
1.8662 0 Td
(2-APB)Tj
3.085 0 Td
(treatment)Tj
4.4032 0 Td
(in)Tj
0.9874 0 Td
(mice)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 252 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Murine)Tj
3.2484 0 Td
(I/R)Tj
1.5137 0 Td
(model)Tj
2.7439 0 Td
(was)Tj
1.7008 0 Td
(generated)Tj
4.1329 0 Td
(following)Tj
3.9798 0 Td
(the)Tj
1.4684 0 Td
(protocol)Tj
3.5999 0 Td
(which)Tj
2.6476 0 Td
(we)Tj
1.3266 0 Td
(have)Tj
2.0636 0 Td
(established)Tj
4.5978 0 Td
(previously)Tj
-33.0234 -1.2982 Td
([)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(].)Tj
2.0749 0 Td
(Briefly,)Tj
3.0727 0 Td
(C57BL/6)Tj
3.7588 0 Td
(male)Tj
2.1429 0 Td
(mice)Tj
2.1543 0 Td
(\(79)Tj
2.024 0 Td
(weeks)Tj
2.6078 0 Td
(old;)Tj
1.7292 0 Td
(Japan)Tj
2.4774 0 Td
(SLC\))Tj
2.2394 0 Td
(were)Tj
2.1203 0 Td
(anesthetized)Tj
5.1647 0 Td
(using)Tj
2.3924 0 Td
(1.5%)Tj
2.1486 0 Td
(iso-)Tj
-36.1074 -1.3039 Td
(flurane)Tj
3.0557 0 Td
(\(Pfizer,)Tj
3.1124 0 Td
(New)Tj
2.0636 0 Td
(York)Tj
2.2224 0 Td
(City,)Tj
2.1316 0 Td
(NY\))Tj
1.9275 0 Td
(carried)Tj
3.0388 0 Td
(in)Tj
1.0374 0 Td
(100%)Tj
2.4038 0 Td
(oxygen)Tj
3.0841 0 Td
(followed)Tj
3.6453 0 Td
(by)Tj
1.1849 0 Td
(the)Tj
1.4683 0 Td
(intubation,)Tj
4.6488 0 Td
(and)Tj
-35.0246 -1.2983 Td
(they)Tj
1.9218 0 Td
(were)Tj
2.126 0 Td
(ventilated)Tj
4.1386 0 Td
(with)Tj
2.0012 0 Td
(100%)Tj
2.4038 0 Td
(oxygen)Tj
3.084 0 Td
(containing)Tj
4.5241 0 Td
(1.5%)Tj
2.1543 0 Td
(isoflurane.)Tj
4.422 0 Td
(After)Tj
2.2848 0 Td
(left-side)Tj
3.4072 0 Td
(thoracot-)Tj
-32.4678 -1.2983 Td
(omy,)Tj
2.228 0 Td
(70)Tj
1.6837 0 Td
(silk)Tj
1.5931 0 Td
(suture)Tj
2.7269 0 Td
(was)Tj
1.7008 0 Td
(tied)Tj
1.7348 0 Td
(around)Tj
3.1407 0 Td
(the)Tj
1.4684 0 Td
(left)Tj
1.474 0 Td
(coronary)Tj
3.8381 0 Td
(artery)Tj
2.5738 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
0.6576 0 Td
(slipknot,)Tj
3.685 0 Td
(which)Tj
2.6533 0 Td
(can)Tj
1.6214 0 Td
(be)Tj
-34.7752 -1.3039 Td
(released)Tj
3.4242 0 Td
(by)Tj
1.1792 0 Td
(pulling)Tj
3.033 0 Td
(one)Tj
1.6951 0 Td
(end)Tj
1.7122 0 Td
(coming)Tj
3.2484 0 Td
(out)Tj
1.5478 0 Td
(from)Tj
2.1996 0 Td
(the)Tj
1.4684 0 Td
(body.)Tj
2.4321 0 Td
(Infarction)Tj
4.2462 0 Td
(was)Tj
1.6895 0 Td
(confirmed)Tj
4.3937 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.4626 0 Td
(discol-)Tj
-34.9112 -1.2982 Td
(oration)Tj
3.1521 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(ventricle.)Tj
3.9061 0 Td
(The)Tj
1.7802 0 Td
(chest)Tj
2.2563 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4684 0 Td
(skin)Tj
1.8935 0 Td
(were)Tj
2.1203 0 Td
(closed)Tj
2.7099 0 Td
(with)Tj
1.9956 0 Td
(50)Tj
1.6894 0 Td
(silk)Tj
1.5931 0 Td
(sutures.)Tj
3.3165 0 Td
(Sham)Tj
2.4775 0 Td
(opera-)Tj
-34.5711 -1.2983 Td
(tion)Tj
1.8425 0 Td
(was)Tj
1.6951 0 Td
(performed)Tj
4.473 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.652 0 Td
(control.)Tj
3.3448 0 Td
(The)Tj
1.7858 0 Td
(mice)Tj
2.1544 0 Td
(were)Tj
2.1203 0 Td
(revived)Tj
3.1407 0 Td
(during)Tj
2.931 0 Td
(the)Tj
1.4684 0 Td
(30-min)Tj
3.1577 0 Td
(ischemic)Tj
3.7304 0 Td
(period,)Tj
-33.5109 -1.2983 Td
(and)Tj
1.7291 0 Td
(anesthetized)Tj
5.1647 0 Td
(shortly)Tj
2.9877 0 Td
(at)Tj
0.9524 0 Td
(the)Tj
1.4683 0 Td
(end)Tj
1.7235 0 Td
(of)Tj
1.0148 0 Td
(ischemia)Tj
3.7417 0 Td
(to)Tj
1.0261 0 Td
(release)Tj
2.9027 0 Td
(the)Tj
1.4683 0 Td
(slipknots,)Tj
4.0479 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.474 0 Td
(heart)Tj
2.2734 0 Td
(was)Tj
-33.7037 -1.3039 Td
(allowed)Tj
3.2881 0 Td
(to)Tj
1.0262 0 Td
(reperfuse)Tj
3.9231 0 Td
(for)Tj
1.3889 0 Td
(24)Tj
1.1736 0 Td
(h.)Tj
0.9694 0 Td
(Using)Tj
2.5965 0 Td
(this)Tj
1.6781 0 Td
(experimental)Tj
5.4595 0 Td
(protocol,)Tj
3.8211 0 Td
(27)Tj
1.1679 0 Td
(out)Tj
1.5534 0 Td
(of)Tj
1.0148 0 Td
(32)Tj
1.1735 0 Td
(mice)Tj
2.1543 0 Td
(survived)Tj
-32.3884 -1.2983 Td
(after)Tj
2.0352 0 Td
(the)Tj
1.4683 0 Td
(surgical)Tj
3.3166 0 Td
(procedure)Tj
4.3143 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(mortality)Tj
3.9175 0 Td
(was)Tj
1.7008 0 Td
(minimized)Tj
4.5694 0 Td
(to)Tj
1.0261 0 Td
(less)Tj
1.6101 0 Td
(than)Tj
2.0296 0 Td
(20%.)Tj
2.1543 0 Td
(Twenty-four)Tj
-31.3396 -1.2982 Td
(hours)Tj
2.5114 0 Td
(after)Tj
2.0353 0 Td
(the)Tj
1.474 0 Td
(reperfusion,)Tj
5.0513 0 Td
(the)Tj
1.4683 0 Td
(mice)Tj
2.1544 0 Td
(were)Tj
2.1203 0 Td
(euthanized)Tj
4.6091 0 Td
(and)Tj
1.7348 0 Td
(the)Tj
1.4683 0 Td
(slipknot)Tj
3.4639 0 Td
(was)Tj
1.6951 0 Td
(retied.)Tj
2.7609 0 Td
(Evans)Tj
2.5852 0 Td
(blue)Tj
-35.1323 -1.3039 Td
(\(5%;)Tj
2.0182 0 Td
(Wako)Tj
2.6192 0 Td
(Pure)Tj
2.1033 0 Td
(Chemical)Tj
4.0252 0 Td
(Industries,)Tj
4.473 0 Td
(Japan\))Tj
2.8233 0 Td
(was)Tj
1.7008 0 Td
(injected)Tj
3.3845 0 Td
(into)Tj
1.8425 0 Td
(the)Tj
1.4684 0 Td
(left)Tj
1.4796 0 Td
(ventricle)Tj
3.6737 0 Td
(\(LV\),)Tj
2.3584 0 Td
(and)Tj
1.7292 0 Td
(the)Tj
-35.6993 -1.2983 Td
(hearts)Tj
2.6362 0 Td
(were)Tj
2.1203 0 Td
(removed.)Tj
3.9741 0 Td
(Isolated)Tj
3.3562 0 Td
(hearts)Tj
2.6362 0 Td
(were)Tj
2.1203 0 Td
(sectioned,)Tj
4.2293 0 Td
(and)Tj
1.7291 0 Td
(viable)Tj
2.5512 0 Td
(myocardium)Tj
5.3744 0 Td
(was)Tj
1.7008 0 Td
(stained)Tj
3.0784 0 Td
(with)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
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f*
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7.4999 0 0 6.6659 218.7212 570.3874 cm
BT
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1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
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1 TL
-0.004 Tc
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(unit.)Tj
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1 j
1 J
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11.9999 0 0 11.9999 200.0125 516.5291 cm
BT
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1 TL
-0.0033 Tc
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4.4504 0 Td
(analysis)Tj
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[()]TJ
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0 Tc
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-0.004 Tc
(0.05.)Tj
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1 j
1 J
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11.9999 0 0 11.9999 200.0125 430.9795 cm
BT
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1 TL
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0 -1.4976 Td
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1 j
1 J
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8.9997 0 0 7.999 278.2488 404.3338 cm
BT
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1 TL
-0.0044 Tc
0 0 Td
(2+)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 291.0047 399.0047 cm
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1 TL
-0.0033 Tc
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(increase)Tj
3.9496 0 Td
(in)Tj
0.9827 0 Td
(NRCMs)Tj
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Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 381.9968 cm
BT
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1 TL
-0.004 Tc
0 0 Td
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2.2677 0 Td
(we)Tj
1.3322 0 Td
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(with)Tj
1.9956 0 Td
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q
1 j
1 J
0 w
7.4999 0 0 6.6659 276.7181 367.3133 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 280.2897 369.0141 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 287.7732 367.3133 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
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10 0 0 10 293.4992 369.0141 cm
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1 TL
-0.004 Tc
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(induced)Tj
3.4695 0 Td
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(Fig)Tj
1.8198 0 Td
(1A)Tj
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(pretreatment)Tj
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(2-APB)Tj
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(1)Tj
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(h)Tj
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1.0205 0 Td
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 372.7559 328.3086 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 376.3275 330.0094 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 383.7543 328.3086 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 389.537 330.0094 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(stimulation)Tj
4.7961 0 Td
(attenuated)Tj
4.4334 0 Td
(H)Tj
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Q
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1 j
1 J
0 w
7.4999 0 0 6.6659 489.4866 328.3086 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 493.0582 330.0094 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 500.5417 328.3086 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 504.0566 330.0094 cm
BT
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1 TL
-0.004 Tc
0 0 Td
(-induced)Tj
3.8154 0 Td
(cell)Tj
-34.2196 -1.2982 Td
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2.4321 0 Td
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1.0318 0 Td
(a)Tj
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(dose-dependent)Tj
6.6273 0 Td
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(showed)Tj
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(almost)Tj
2.8856 0 Td
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(inhibition)Tj
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(cell)Tj
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(death)Tj
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(at)Tj
-35.0926 -1.3039 Td
(100)Tj
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1.6441 0 Td
[()]TJ
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(M)Tj
1.6214 0 Td
(\(cell)Tj
1.8992 0 Td
(death,)Tj
2.6589 0 Td
(10.8)Tj
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[()]TJ
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(4.4%)Tj
4.371 0 Td
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1.0375 0 Td
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4.3086 0 Td
[()2()]TJ
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1.2076 0 Td
(89.2)Tj
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[()]TJ
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(16.7%)Tj
4.8528 0 Td
(in)Tj
1.0375 0 Td
(non-pretreated)Tj
6.2644 0 Td
(samples;)Tj
0.83 0.64 0.02 0 k
3.6057 0 Td
(Fig)Tj
-34.5087 -1.2983 Td
(1A)Tj
0 g
(\).)Tj
1.9446 0 Td
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(previous)Tj
3.651 0 Td
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3.0671 0 Td
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(that)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 376.4409 289.3606 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 379.9559 291.0047 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 387.4393 289.3606 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 391.011 291.0047 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(-induced)Tj
3.8154 0 Td
(cell)Tj
1.559 0 Td
(death)Tj
2.4321 0 Td
(is)Tj
0.8504 0 Td
(associated)Tj
4.2519 0 Td
(with)Tj
2.0013 0 Td
(the)Tj
-34.0098 -1.2982 Td
(impaired)Tj
3.8438 0 Td
(Ca)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 249.4488 282.444 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 259.5401 278.022 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(homeostasis)Tj
5.0853 0 Td
(in)Tj
1.0374 0 Td
(other)Tj
2.3471 0 Td
(cell)Tj
1.5591 0 Td
(types)Tj
2.279 0 Td
([)Tj
0.83 0.64 0.02 0 k
(32)Tj
0 g
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1.3266 0 Td
(assessed)Tj
3.4752 0 Td
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5.057 0 Td
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q
1 j
1 J
0 w
7.4999 0 0 6.6659 512.9007 282.444 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 522.9921 278.022 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(concen-)Tj
-32.2977 -1.3039 Td
(trations)Tj
3.3052 0 Td
(during)Tj
2.9253 0 Td
(H)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 270.0283 263.3385 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 273.5433 264.9826 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 281.0267 263.3385 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 286.8094 264.9826 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(treatment)Tj
4.1328 0 Td
(with)Tj
1.9956 0 Td
(or)Tj
1.0999 0 Td
(without)Tj
3.3335 0 Td
(2-APB)Tj
2.8686 0 Td
(pretreatment)Tj
5.4482 0 Td
(in)Tj
1.0318 0 Td
(NRCMs.)Tj
3.7247 0 Td
(A)Tj
0.9014 0 Td
(consi-)Tj
-33.2161 -1.2982 Td
(derable)Tj
3.1521 0 Td
(increase)Tj
3.4696 0 Td
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(intracellular)Tj
5.0569 0 Td
(Ca)Tj
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Q
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1 j
1 J
0 w
7.4999 0 0 6.6659 338.1732 256.422 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 348.2645 252 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(concentration)Tj
5.8166 0 Td
(was)Tj
1.7008 0 Td
(observed)Tj
3.7984 0 Td
(within)Tj
2.8119 0 Td
(10)Tj
1.1679 0 Td
(min)Tj
1.8595 0 Td
(after)Tj
2.0353 0 Td
(H)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 547.8236 250.3559 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 551.3952 252 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 558.822 250.3559 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 239.0173 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(treatment,)Tj
4.3596 0 Td
(which)Tj
2.6475 0 Td
(was)Tj
1.7008 0 Td
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4.5808 0 Td
(abolished)Tj
4.0195 0 Td
(by)Tj
1.1849 0 Td
(2-APB)Tj
2.8686 0 Td
(pretreatment)Tj
5.4482 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1B)Tj
0 g
(\).)Tj
1.8425 0 Td
(To)Tj
1.3379 0 Td
(elucidate)Tj
-31.8101 -1.3039 Td
(the)Tj
1.4683 0 Td
(relationship)Tj
4.9889 0 Td
(between)Tj
3.5263 0 Td
(the)Tj
1.474 0 Td
(2-APB-mediated)Tj
6.9392 0 Td
(cell)Tj
1.559 0 Td
(death)Tj
2.4321 0 Td
(inhibition)Tj
4.2236 0 Td
(and)Tj
1.7292 0 Td
(the)Tj
1.474 0 Td
(prevention)Tj
4.5807 0 Td
(of)Tj
1.0148 0 Td
(an)Tj
-35.4101 -1.2982 Td
(increase)Tj
3.4695 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(intracellular)Tj
5.057 0 Td
(Ca)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 321.3354 217.4173 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 331.4267 212.9952 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(levels,)Tj
2.6021 0 Td
(we)Tj
1.3266 0 Td
(used)Tj
2.058 0 Td
(BAPTA-AM,)Tj
5.4935 0 Td
(an)Tj
1.2019 0 Td
(intracellular)Tj
5.057 0 Td
(Ca)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 519.8173 217.4173 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 529.9086 212.9952 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(chelator,)Tj
3.6736 0 Td
(to)Tj
-36.6629 -1.2982 Td
(analyze)Tj
3.1747 0 Td
(whether)Tj
3.4753 0 Td
(the)Tj
1.4683 0 Td
(reduction)Tj
4.1159 0 Td
(of)Tj
1.0148 0 Td
(Ca)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 343.4456 204.4346 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2+)Tj
ET
Q
q
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