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Luis Jose Rene Arredondo-Hernandez, Carlos Diaz-Avalos, Yolanda Lopez-Vidal, Gonzalo Castillo-Rojas, Marisa Mazari-Hiriart
FRNA Bacteriophages as Viral Indicators of Faecal Contamination in Mexican Tropical Aquatic Systems
www.plosone.org
www.plosone.org
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3.5659 0 Td
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4.9153 0 Td
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1.7518 0 Td
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(real-time)Tj
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1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 517.5496 cm
BT
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1 TL
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Q
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-33.4712 -1.3039 Td
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3.2995 0 Td
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3.2088 0 Td
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1.7292 0 Td
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1.4741 0 Td
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2.0579 0 Td
(faecal)Tj
2.4718 0 Td
(contamination)Tj
6.1171 0 Td
(and)Tj
1.7348 0 Td
(conditions)Tj
4.473 0 Td
(that)Tj
1.7802 0 Td
(facilitate)Tj
3.6 0 Td
(the)Tj
1.4683 0 Td
(establishment)Tj
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(of)Tj
1.0148 0 Td
(microbial)Tj
4.0592 0 Td
(niches.)Tj
-34.5144 -1.2982 Td
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1.4626 0 Td
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3.1918 0 Td
(permissions)Tj
5.023 0 Td
(were)Tj
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(required)Tj
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1.0262 0 Td
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2.2847 0 Td
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2.2563 0 Td
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3.8098 0 Td
(because)Tj
3.3222 0 Td
(they)Tj
1.9218 0 Td
(are)Tj
1.4514 0 Td
(natural)Tj
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-34.5881 -1.2983 Td
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1.0147 0 Td
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2.4322 0 Td
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1.9955 0 Td
(open)Tj
2.2167 0 Td
(access.)Tj
2.8687 0 Td
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2.2166 0 Td
(studies)Tj
2.9821 0 Td
(did)Tj
1.542 0 Td
(not)Tj
1.5761 0 Td
(involve)Tj
3.1237 0 Td
(endangered)Tj
4.9096 0 Td
(or)Tj
1.0998 0 Td
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4.0139 0 Td
(species)Tj
2.9933 0 Td
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-34.9849 -1.2983 Td
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4.6261 0 Td
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2.5795 0 Td
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(a)Tj
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4.5354 0 Td
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2.9424 0 Td
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1.0375 0 Td
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1.4683 0 Td
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1.8822 0 Td
(surrounding)Tj
5.2441 0 Td
(Mexico)Tj
3.1974 0 Td
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-34.7468 -1.3039 Td
(flows)Tj
2.3073 0 Td
(through)Tj
3.4413 0 Td
(a)Tj
0.6576 0 Td
(forested)Tj
3.4186 0 Td
(area)Tj
1.8822 0 Td
(and)Tj
1.7291 0 Td
(is)Tj
0.8504 0 Td
(used)Tj
2.0579 0 Td
(as)Tj
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(a)Tj
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(source)Tj
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(for)Tj
1.389 0 Td
(drinking)Tj
3.7133 0 Td
(water)Tj
2.4265 0 Td
(production.)Tj
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-33.3182 -1.2983 Td
(entering)Tj
3.5659 0 Td
(the)Tj
1.4683 0 Td
(urban)Tj
2.6079 0 Td
(area,)Tj
2.109 0 Td
(the)Tj
1.4683 0 Td
(river)Tj
2.109 0 Td
(receives)Tj
3.3675 0 Td
(municipal)Tj
4.2746 0 Td
(wastewater)Tj
4.6318 0 Td
(discharges)Tj
4.3823 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
2.075 0 Td
(In)Tj
1.1055 0 Td
(contrast,)Tj
-33.1651 -1.2982 Td
(the)Tj
1.4683 0 Td
(Cuitzmala)Tj
4.3256 0 Td
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2.3584 0 Td
(water)Tj
2.4265 0 Td
(system)Tj
2.9423 0 Td
(flows)Tj
2.3017 0 Td
(through)Tj
3.4469 0 Td
(a)Tj
0.652 0 Td
(tropical)Tj
3.2882 0 Td
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1.8822 0 Td
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1.9956 0 Td
(a)Tj
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(small)Tj
2.3244 0 Td
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4.5921 0 Td
(and)Tj
1.7291 0 Td
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-36.3909 -1.3039 Td
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3.0784 0 Td
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1.2755 0 Td
(the)Tj
1.4684 0 Td
(coast)Tj
2.245 0 Td
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1.0375 0 Td
(Jalisco)Tj
2.7892 0 Td
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2.1487 0 Td
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1.0318 0 Td
(Western)Tj
3.617 0 Td
(Mexico)Tj
3.1974 0 Td
([)Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(].)Tj
2.075 0 Td
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1.7801 0 Td
(Xochimilco)Tj
4.8643 0 Td
(wetland)Tj
3.3845 0 Td
(is)Tj
0.8447 0 Td
(remi-)Tj
-34.8375 -1.2983 Td
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3.0897 0 Td
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1.0148 0 Td
(the)Tj
1.4683 0 Td
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3.1975 0 Td
(basin)Tj
2.3414 0 Td
(lacustrine)Tj
4.1215 0 Td
(system)Tj
2.9367 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(];)Tj
2.075 0 Td
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0.788 0 Td
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0.8447 0 Td
(a)Tj
0.6576 0 Td
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3.8721 0 Td
(aquatic)Tj
3.1125 0 Td
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1.9955 0 Td
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2.9991 0 Td
(wastewater)Tj
4.6317 0 Td
(and)Tj
1.7292 0 Td
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3.3675 0 Td
(storm)Tj
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(rainy)Tj
2.296 0 Td
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3.0784 0 Td
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-34.1402 -1.3039 Td
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2.1089 0 Td
(agriculture)Tj
4.5751 0 Td
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0.8447 0 Td
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3.9061 0 Td
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1.0375 0 Td
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(known)Tj
3.0047 0 Td
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1.0148 0 Td
(the)Tj
1.4683 0 Td
(Mezquital)Tj
4.2293 0 Td
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2.7212 0 Td
(\(Hidalgo)Tj
3.7757 0 Td
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-32.1446 -1.2982 Td
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3.7643 0 Td
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2.1884 0 Td
(a)Tj
0.6576 0 Td
(significant)Tj
4.388 0 Td
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4.6318 0 Td
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1.0148 0 Td
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1.4683 0 Td
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4.269 0 Td
(wastewater)Tj
4.6318 0 Td
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2.211 0 Td
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1.9049 0 Td
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0.8447 0 Td
(dis-)Tj
-35.172 -1.2983 Td
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3.3902 0 Td
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3.3335 0 Td
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4.1329 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(].)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 242.9858 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
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ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 225.9779 cm
BT
/F5 1 Tf
1 TL
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4.439 0 Td
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1.0204 0 Td
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1.474 0 Td
(faecal)Tj
2.4662 0 Td
(enterococci)Tj
4.8359 0 Td
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-35.6596 -1.2982 Td
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1.0941 0 Td
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2.0636 0 Td
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1.0375 0 Td
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3.7644 0 Td
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1.0375 0 Td
(autoclaved)Tj
4.4957 0 Td
(polypropylene)Tj
5.9584 0 Td
(bottles,)Tj
3.084 0 Td
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-34.2082 -1.2983 Td
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2.1203 0 Td
(then)Tj
2.0182 0 Td
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1.0262 0 Td
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1.4683 0 Td
(laboratory)Tj
4.3483 0 Td
(at)Tj
0.9525 0 Td
(4C)Tj
1.7518 0 Td
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1.3833 0 Td
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2.2053 0 Td
(concentration)Tj
5.8167 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(S2)Tj
1.5136 0 Td
(Table)Tj
0 g
(\).)Tj
3.0048 0 Td
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2.3697 0 Td
(the)Tj
-34.8602 -1.3039 Td
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3.3788 0 Td
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2.126 0 Td
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4.1329 0 Td
(following)Tj
3.9798 0 Td
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3.7247 0 Td
(procedures)Tj
4.6715 0 Td
(within)Tj
2.8119 0 Td
(6)Tj
0.6973 0 Td
(h)Tj
0.7484 0 Td
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1.0148 0 Td
(collection)Tj
4.1102 0 Td
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1.0148 0 Td
(described)Tj
4.0535 0 Td
(by)Tj
-36.4646 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
2.0749 0 Td
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1.7801 0 Td
(20-L)Tj
2.0637 0 Td
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2.4321 0 Td
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3.3788 0 Td
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2.126 0 Td
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1.1792 0 Td
(ultrafiltration)Tj
5.6239 0 Td
(with)Tj
1.9956 0 Td
(polycarbonate)Tj
5.896 0 Td
(ultra-fil-)Tj
-33.9985 -1.2982 Td
(ters)Tj
1.6724 0 Td
(\(Hemoflow)Tj
4.8018 0 Td
(F80A,)Tj
2.6079 0 Td
(Fresenius)Tj
4.0308 0 Td
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3.4356 0 Td
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2.3414 0 Td
(Waltham,)Tj
4.1669 0 Td
(MA,)Tj
2.0239 0 Td
(USA\),)Tj
2.6816 0 Td
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2.3924 0 Td
(a)Tj
0.652 0 Td
(perfusion)Tj
4.0308 0 Td
(rate)Tj
1.7462 0 Td
(of)Tj
-36.5837 -1.2983 Td
(1700)Tj
2.1203 0 Td
(mL/min,)Tj
3.77 0 Td
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1.0148 0 Td
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4.0535 0 Td
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1.1792 0 Td
(Hill)Tj
1.7462 0 Td
(et)Tj
0.9411 0 Td
(al.)Tj
1.1281 0 Td
([)Tj
0.83 0.64 0.02 0 k
(28)Tj
0 g
(])Tj
1.8482 0 Td
(while)Tj
2.3698 0 Td
(adenovirus)Tj
4.6431 0 Td
(detection)Tj
3.9401 0 Td
(required)Tj
3.6397 0 Td
(further)Tj
3.0274 0 Td
(con-)Tj
-35.4215 -1.3039 Td
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4.3369 0 Td
(\(10,000)Tj
3.1635 0 Td
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1.44 0 Td
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2.3924 0 Td
(Amicon)Tj
3.4696 0 Td
(centrifugal)Tj
4.5127 0 Td
(units)Tj
2.2224 0 Td
(\(30,000)Tj
3.1634 0 Td
(MWCO;)Tj
3.7134 0 Td
(Merck)Tj
2.8289 0 Td
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4.1839 0 Td
(Bil-)Tj
-35.4271 -1.2983 Td
(lerica,)Tj
2.6078 0 Td
(MA,)Tj
2.0239 0 Td
(USA\).)Tj
2.6816 0 Td
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2.5568 0 Td
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1.4684 0 Td
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5.4482 0 Td
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1.0261 0 Td
(a)Tj
0.652 0 Td
(200-mL)Tj
3.3562 0 Td
(final)Tj
2.0069 0 Td
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3.4242 0 Td
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1.7291 0 Td
(stored)Tj
-34.486 -1.2982 Td
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1.0318 0 Td
(40-mL)Tj
2.8799 0 Td
(aliquots.)Tj
3.5887 0 Td
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4.8869 0 Td
(RNA)Tj
2.262 0 Td
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1.6951 0 Td
(extracted)Tj
3.8835 0 Td
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2.2053 0 Td
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1.4683 0 Td
(100X)Tj
2.2961 0 Td
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1.0261 0 Td
(eval-)Tj
-35.6879 -1.304 Td
(uate)Tj
1.9048 0 Td
(the)Tj
1.4684 0 Td
(FRNA)Tj
2.7892 0 Td
(bacteriophage)Tj
5.8167 0 Td
(genotypic)Tj
4.1272 0 Td
(groups)Tj
2.9707 0 Td
(present)Tj
3.1634 0 Td
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2.3868 0 Td
(RT-qPCR.)Tj
4.3823 0 Td
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4.8926 0 Td
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2.1203 0 Td
(fro-)Tj
-36.0224 -1.2982 Td
(zen)Tj
1.6043 0 Td
(and)Tj
1.7292 0 Td
(stored)Tj
2.7099 0 Td
(at)Tj
/F10 1 Tf
0.9524 0 Td
[()]TJ
/F5 1 Tf
(70C)Tj
2.795 0 Td
([)Tj
0.83 0.64 0.02 0 k
(29)Tj
0 g
(],)Tj
2.0749 0 Td
(after)Tj
2.0353 0 Td
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