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Ana C. Bahia, Marina S. Kubota, Jayme A. Souza-Neto, Leonardo B. Koerich, Ana Beatriz Barletta, Helena R. C. Araújo, Caroline M. Gonçalves, Cláudia M. Ríos-Velásquez, Paulo F. P. Pimenta, Yara M. Traub-Csekö
An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteria
www.plosntds.org
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5.76 0 Td
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[()30()6()14(!)13()-20()]TJ
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1.1792 0 Td
[()]TJ
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[( )-13()15( )3(\n)]TJ
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(infection)Tj
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(that)Tj
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(the)Tj
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6.2022 0 Td
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10.3974 0 Td
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5.2044 0 Td
(that)Tj
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3.4073 0 Td
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1.4683 0 Td
[()]TJ
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3.8437 0 Td
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3.6227 0 Td
(response)Tj
3.736 0 Td
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3.0274 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[( )-13()15( )3(\n)]TJ
/F10 1 Tf
2.3074 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.474 0 Td
(first)Tj
1.8028 0 Td
(evidence)Tj
3.7134 0 Td
(of)Tj
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1.6781 0 Td
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Q
q
1 j
1 J
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11.9999 0 0 11.9999 200.0125 486.7086 cm
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/F3 1 Tf
1 TL
-0.0033 Tc
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/F17 1 Tf
0 -1.5023 Td
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4.2189 0 Td
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3.2976 0 Td
(and)Tj
1.7953 0 Td
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Q
q
1 j
1 J
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BT
/F15 1 Tf
1 TL
-0.004 Tc
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[()]TJ
/F10 1 Tf
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1.0941 0 Td
[()6()14()4()-3()11()12()-4()]TJ
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3.8438 0 Td
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3.1748 0 Td
(1932\))Tj
2.4661 0 Td
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(a)Tj
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4.0705 0 Td
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(].)Tj
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2.0012 0 Td
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/F15 1 Tf
4.6148 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[( )-13()15( )3(\n)]TJ
/F10 1 Tf
2.3074 0 Td
(malaria)Tj
-33.3635 -1.2983 Td
(patients)Tj
3.3675 0 Td
(were)Tj
2.1203 0 Td
(conducted)Tj
4.4277 0 Td
(in)Tj
1.0375 0 Td
(Manaus,)Tj
3.6453 0 Td
(a)Tj
0.6519 0 Td
(Brazilian)Tj
3.7814 0 Td
(endemic)Tj
3.651 0 Td
(area)Tj
1.8822 0 Td
(for)Tj
1.389 0 Td
(malaria,)Tj
3.4526 0 Td
(as)Tj
1.0148 0 Td
(described)Tj
4.0535 0 Td
(in)Tj
1.0318 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(],)Tj
-35.5065 -1.2982 Td
(in)Tj
1.0318 0 Td
(accordance)Tj
4.7395 0 Td
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1.9955 0 Td
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3.4243 0 Td
(guidelines)Tj
4.2746 0 Td
(and)Tj
1.7291 0 Td
(regulations.)Tj
4.8982 0 Td
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4.0422 0 Td
(consent)Tj
3.3335 0 Td
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1.7008 0 Td
(obtained)Tj
3.7304 0 Td
(from)Tj
-34.8999 -1.304 Td
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1.1451 0 Td
(subjects.)Tj
3.6057 0 Td
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/F15 1 Tf
3.0727 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9978 0 Td
[( )-13()15( )3(\n)]TJ
/F10 1 Tf
2.6362 0 Td
(malaria)Tj
3.2258 0 Td
(patients)Tj
3.3676 0 Td
(were)Tj
2.1203 0 Td
(diagnosed)Tj
4.2802 0 Td
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(microscopic)Tj
5.108 0 Td
(examination)Tj
5.2327 0 Td
(of)Tj
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6.4516 0 Td
(blood)Tj
2.5114 0 Td
(smears.)Tj
3.2145 0 Td
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1.7858 0 Td
(mosquitoes)Tj
4.7962 0 Td
(were)Tj
2.126 0 Td
(artificially)Tj
4.2179 0 Td
(fed)Tj
1.457 0 Td
(on)Tj
1.2756 0 Td
(infected)Tj
3.4186 0 Td
(or)Tj
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(blood)Tj
2.5114 0 Td
(at)Tj
0.9525 0 Td
(37C)Tj
2.228 0 Td
(constant)Tj
3.6396 0 Td
(temperature,)Tj
5.3518 0 Td
(maintained)Tj
4.7905 0 Td
(using)Tj
2.3925 0 Td
(a)Tj
0.6519 0 Td
(water)Tj
2.4321 0 Td
(circulation)Tj
4.5354 0 Td
(system,)Tj
3.1635 0 Td
(to)Tj
1.0205 0 Td
(prevent)Tj
-33.6697 -1.3039 Td
(exflagellation)Tj
5.5105 0 Td
(of)Tj
1.0148 0 Td
(microgametocytes.)Tj
7.7442 0 Td
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2.2847 0 Td
(the)Tj
1.4683 0 Td
(artificial)Tj
3.515 0 Td
(feeding,)Tj
3.3902 0 Td
(the)Tj
1.4683 0 Td
(infected)Tj
3.4242 0 Td
(and)Tj
1.7292 0 Td
(non-infected)Tj
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(mosquitoes)Tj
4.7961 0 Td
(were)Tj
2.126 0 Td
(kept)Tj
1.9559 0 Td
(in)Tj
1.0318 0 Td
(cages)Tj
2.3301 0 Td
(and)Tj
1.7291 0 Td
(given)Tj
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(10%)Tj
1.9275 0 Td
(sucrose)Tj
/F15 1 Tf
3.1918 0 Td
[()6(\r)]TJ
1.2019 0 Td
[()12()14()11()14()7()12()]TJ
/F10 1 Tf
(.)Tj
3.2995 0 Td
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1.3946 0 Td
(experimental)Tj
5.4595 0 Td
(protocols)Tj
-32.8249 -1.2982 Td
(were)Tj
2.1203 0 Td
(approved)Tj
3.9741 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.474 0 Td
(Brazilian)Tj
3.7814 0 Td
(Ministry)Tj
3.6794 0 Td
(of)Tj
1.0147 0 Td
(Health,)Tj
3.1408 0 Td
(National)Tj
3.6907 0 Td
(Council)Tj
3.4072 0 Td
(of)Tj
1.0148 0 Td
(Health,)Tj
3.1408 0 Td
(and)Tj
1.7291 0 Td
(National)Tj
-33.3465 -1.304 Td
(Committee)Tj
4.7394 0 Td
(of)Tj
1.0148 0 Td
(Ethics)Tj
2.6646 0 Td
(in)Tj
1.0375 0 Td
(Research)Tj
3.804 0 Td
(\(CONEP\),)Tj
4.4051 0 Td
(written)Tj
3.1067 0 Td
(approval)Tj
3.702 0 Td
(number)Tj
3.4186 0 Td
(3726.)Tj
-26.6965 -1.2982 Td
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1.3379 0 Td
(infect)Tj
2.4718 0 Td
(mosquitoes)Tj
4.7962 0 Td
(with)Tj
2.0012 0 Td
(the)Tj
1.4684 0 Td
(Gram-positive)Tj
/F15 1 Tf
6.0094 0 Td
[(!)10()11()12()10()24(")3()4()4()7()4()7()1()-4()]TJ
6.0094 0 Td
[()16()12()20()2()-4()]TJ
/F10 1 Tf
2.846 0 Td
(or)Tj
1.0998 0 Td
(Gram-negative)Tj
/F15 1 Tf
-29.2363 -1.2983 Td
[(#)16()22()11()2()20()13()4()6()-4()11()2()]TJ
5.1987 0 Td
[()11()12()-9()4()6()]TJ
/F10 1 Tf
(,)Tj
3.77 0 Td
(one)Tj
1.6951 0 Td
(OD)Tj
1.7065 0 Td
(600)Tj
1.644 0 Td
(of)Tj
1.0148 0 Td
(bacterial)Tj
3.6284 0 Td
(culture)Tj
3.0387 0 Td
(growth)Tj
3.0727 0 Td
(overnight)Tj
4.0819 0 Td
(\(approximately)Tj
6.3155 0 Td
(10)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 561.2031 300.1322 cm
BT
/F10 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(9)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 282.7275 cm
BT
/F10 1 Tf
1 TL
-0.004 Tc
0 0 Td
(bacteria)Tj
3.3788 0 Td
(ml)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 244.4598 287.1496 cm
BT
/F10 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F10 1 Tf
1 TL
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(\))Tj
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1.7008 0 Td
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3.1805 0 Td
(twice)Tj
2.313 0 Td
(in)Tj
1.0375 0 Td
(phosphate-buffered)Tj
8.039 0 Td
(saline)Tj
2.5001 0 Td
(\(PBS\))Tj
2.5172 0 Td
(and)Tj
1.7291 0 Td
(resuspended)Tj
5.2327 0 Td
(in)Tj
1.0375 0 Td
(1)Tj
0.6973 0 Td
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-35.6028 -1.3039 Td
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1.0148 0 Td
(bicarbonate-buffered)Tj
8.6455 0 Td
(saline-agarose)Tj
5.845 0 Td
(\(BBSA\))Tj
3.2202 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(].)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 231.0236 cm
BT
/F17 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(RACE)Tj
2.8299 0 Td
(and)Tj
1.7906 0 Td
(searching)Tj
4.2094 0 Td
(for)Tj
1.4693 0 Td
(GATA)Tj
2.9953 0 Td
(sequences)Tj
4.3418 0 Td
(in)Tj
1.0677 0 Td
(the)Tj
/F18 1 Tf
1.5402 0 Td
[()]TJ
/F17 1 Tf
(.)Tj
/F18 1 Tf
1.1291 0 Td
[()5()11()-1()-7()-7()9()5()-13()]TJ
/F17 1 Tf
4.0111 0 Td
(partial)Tj
-25.3844 -1.1669 Td
(genome)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 200.0125 cm
BT
/F10 1 Tf
1 TL
-0.004 Tc
0 0 Td
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1.3322 0 Td
(obtain)Tj
2.778 0 Td
(the)Tj
1.4626 0 Td
(5')Tj
0.9071 0 Td
(and)Tj
1.7235 0 Td
(3')Tj
0.907 0 Td
(ends)Tj
2.075 0 Td
(of)Tj
1.0091 0 Td
(the)Tj
1.4627 0 Td
(GATA)Tj
2.914 0 Td
(cDNA,)Tj
3.016 0 Td
(the)Tj
1.4684 0 Td
(SMART)Tj
3.4979 0 Td
(cDNA)Tj
2.7949 0 Td
(RACE)Tj
2.7553 0 Td
(\(Becton)Tj
3.3335 0 Td
(Dickinson)Tj
-33.4372 -1.3039 Td
(Clontech\))Tj
4.1952 0 Td
(amplification)Tj
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(technique)Tj
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1.7007 0 Td
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(amplicons)Tj
4.3483 0 Td
(generated)Tj
4.1329 0 Td
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1.1792 0 Td
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(RACE)Tj
2.7553 0 Td
(PCR)Tj
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(reac-)Tj
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3.8778 0 Td
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(\(Promega\))Tj
4.4844 0 Td
(and)Tj
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(to)Tj
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(transform)Tj
4.2179 0 Td
(competent)Tj
/F15 1 Tf
-32.5358 -1.2983 Td
[(#)-4()-3()13()6()2()-9()4()13()18()4()]TJ
4.6261 0 Td
[()7()10()12()]TJ
/F10 1 Tf
1.559 0 Td
(DH5)Tj
/F16 1 Tf
()Tj
/F10 1 Tf
(.)Tj
2.9934 0 Td
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3.7984 0 Td
(were)Tj
2.1203 0 Td
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(in)Tj
1.0375 0 Td
(an)Tj
1.2018 0 Td
(ABI)Tj
1.8199 0 Td
(3700)Tj
2.1259 0 Td
(sequencer)Tj
4.2293 0 Td
(\(Applied)Tj
3.7417 0 Td
(Biosys-)Tj
-33.6413 -1.2983 Td
(tems\))Tj
2.4604 0 Td
(at)Tj
0.9524 0 Td
(the)Tj
1.4684 0 Td
(PDTIS/FIOCRUZ)Tj
7.5117 0 Td
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4.8302 0 Td
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3.9685 0 Td
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1.7802 0 Td
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4.2179 0 Td
(obtained)Tj
3.736 0 Td
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(to)Tj
-35.1097 -1.3039 Td
(mount)Tj
2.9196 0 Td
(the)Tj
1.4684 0 Td
(cDNAs)Tj
3.1634 0 Td
(of)Tj
/F15 1 Tf
1.0148 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
1.0998 0 Td
[()6()14()4()-3()11()12()-4()]TJ
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3.8438 0 Td
(GATA)Tj
2.914 0 Td
(with)Tj
1.9956 0 Td
(the)Tj
1.4683 0 Td
(CAP3)Tj
2.6022 0 Td
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3.9741 0 Td
(Assembly)Tj
4.0762 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(http://pbil.univ-)Tj
-30.5402 -1.2982 Td
(lyon1.fr/cap3.php)Tj
0 g
7.302 0 Td
(\))Tj
0.5612 0 Td
(and)Tj
1.7291 0 Td
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2.9991 0 Td
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(\()Tj
0.83 0.64 0.02 0 k
(http://www.ebi.ac.uk/Tools/clus)Tj
13.1243 0 Td
(talw2/)Tj
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(\).)Tj
3.2485 0 Td
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1.8028 0 Td
(GATA)Tj
2.914 0 Td
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3.8607 0 Td
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1.7008 0 Td
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2.058 0 Td
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1.0261 0 Td
(search)Tj
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(for)Tj
1.3833 0 Td
(GATA)Tj
2.9197 0 Td
(sequences)Tj
4.2179 0 Td
(in)Tj
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(the)Tj
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1.474 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
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1.0942 0 Td
[()6()14()4()-3()11()12()-4()]TJ
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3.8437 0 Td
(genome.)Tj
3.634 0 Td
(This)Tj
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2.8913 0 Td
(Genome)Tj
3.6566 0 Td
(Shotgun)Tj
3.566 0 Td
(project)Tj
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1.6951 0 Td
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4.0762 0 Td
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0.9525 0 Td
(DDBJ/ENA/GenBank)Tj
8.9971 0 Td
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2.6192 0 Td
(the)Tj
1.4683 0 Td
(accession)Tj
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(number)Tj
3.4185 0 Td
(NJHH00000000.)Tj
6.8485 0 Td
(Version)Tj
3.3959 0 Td
(NJHH01000000)Tj
6.6216 0 Td
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1.7008 0 Td
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2.058 0 Td
(in)Tj
1.0374 0 Td
(this)Tj
1.6725 0 Td
(paper.)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 183.2882 23.6976 re
W* n
q
183.2315 0 0 23.6409 36 741.3165 cm
q
/I1 Do
Q
Q
Q
q
0 0 612 792 re
W* n
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1 J
0 w
7.9999 0 0 7.9999 343.7858 745.7952 cm
BT
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q
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[()]TJ
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-31.3849 -1.2983 Td
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1.7121 0 Td
(granulocytes\).)Tj
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1.7631 0 Td
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3.8438 0 Td
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3.4355 0 Td
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(GraphPad)Tj
4.2633 0 Td
(Prism)Tj
2.5682 0 Td
(5)Tj
0.6859 0 Td
(\(GraphPad,)Tj
4.8132 0 Td
(La)Tj
1.1792 0 Td
(Jolla,)Tj
-35.6085 -1.3039 Td
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(USA\))Tj
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2.1826 0 Td
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2.2337 0 Td
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5.2101 0 Td
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4.0308 0 Td
(replicates.)Tj
4.1442 0 Td
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4.2917 0 Td
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3.736 0 Td
(and)Tj
1.7121 0 Td
(propor-)Tj
-34.3783 -1.2983 Td
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2.1713 0 Td
(analyses)Tj
3.4355 0 Td
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3.8438 0 Td
(t-test)Tj
2.211 0 Td
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1.7121 0 Td
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4.8642 0 Td
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3.4242 0 Td
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0 Tc
(<)Tj
/F10 1 Tf
-0.01 Tc
(0.05.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 209.0267 cm
BT
/F3 1 Tf
1 TL
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0 0 Td
(Results)Tj
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0 -1.5023 Td
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1.4693 0 Td
[()]TJ
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(.)Tj
/F18 1 Tf
1.1292 0 Td
[()5()11()-1()-7()-7()9()5()-13()]TJ
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4.0063 0 Td
(GATA)Tj
2.9953 0 Td
(TFs)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 173.9905 cm
BT
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1 TL
-0.004 Tc
0 0 Td
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2.5738 0 Td
(GATA)Tj
2.9197 0 Td
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4.6374 0 Td
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3.5262 0 Td
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[()]TJ
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(.)Tj
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1.0999 0 Td
[()6()14()4()-3()11()12()-4()]TJ
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3.8437 0 Td
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4.4957 0 Td
(and)Tj
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-35.5915 -1.2983 Td
[()]TJ
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(.)Tj
/F15 1 Tf
0.9921 0 Td
[( )-13()15( )3(\n)]TJ
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2.3074 0 Td
(infected)Tj
3.4185 0 Td
(blood)Tj
2.5172 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
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1.7858 0 Td
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3.5036 0 Td
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[()6()14()4()-3()11()12()-4()]TJ
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1.7348 0 Td
(AGAP002235-PA\))Tj
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ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 183.2882 23.6976 re
W* n
q
183.2315 0 0 23.6409 36 741.3165 cm
q
/I1 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 343.7858 745.7952 cm
BT
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0.978 0 Td
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4.8615 0 Td
(aquasal)Tj
3.4299 0 Td
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0.9284 0 Td
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ET
Q
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36 48.0002 m
576 48.0002 l
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36 47.5002 l
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0.83 0.64 0.02 0 k
163.5591 34.9228 m
317.7071 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
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4.6417 0 Td
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3.7135 0 Td
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