Cryopreservation of rat hippocampal slices by vitrification.
@article{Pichugin2006CryopreservationOR, title={Cryopreservation of rat hippocampal slices by vitrification.}, author={Yu. P. Pichugin and Gregory M Fahy and Robert B. Morin}, journal={Cryobiology}, year={2006}, volume={52 2}, pages={ 228-40 }, url={https://api.semanticscholar.org/CorpusID:13007686} }
47 Citations
Cryopreservation of precision-cut tissue slices
- 2013
Medicine
Cryopreservation of precision-cut tissue slices (PCTS) would have many advantages for drug development and would encourage more extensive use of the PCTS preparation, but more attention is needed to the effects of Cryobiological principles on specific cell types within PCTs and to the general integrity and viability of cryopreserved P CTS.
Scientific Justification of Cryonics Practice
- 2008
Medicine
Under ideal conditions, the time interval between onset of clinical death and beginning of cryonics procedures can be reduced to less than 1 min, but much longer delays could also be compatible with ultimate survival, with life-saving results for anyone with medical needs that exceed current capabilities.
Critical factors in vitrification-based cryopreservation of precision-cut liver slices
Medicine
The empirical literature on the cryopreservation of PCTS has not taken sufficient account of cryobiological principles, and may, for example, include the effects of easily preventable osmotic injury.
Critical factors in vitrification-based cryopreservation of precision-cut liver slices
Medicine
The empirical literature on the cryopreservation of PCTS has not taken sufficient account of cryobiological principles, and may, for example, include the effects of easily preventable osmotic injury.
Critical factors in vitrification-based cryopreservation of precision-cut liver slices
Medicine
The empirical literature on the cryopreservation of PCTS has not taken sufficient account of cryobiological principles, and may, for example, include the effects of easily preventable osmotic injury.
Freezing and Thawing Characteristics and Post-Thaw Viability of Differentiated PC12 Cells Adhered to Substrate
- 2013
Biology, Materials Science
The freezing of adherent neuron-like cells with neurites, which are necessary to form neuronal network, in physiological saline were investigated to understand their basic characteristics on the freezing and thawing.
Chapter 2 Principles of Cryopreservation by Vitrification
- 2019
Medicine, Biology
This chapter describes the basic principles of vitrification and indicates the broad potential biological relevance of vitrine and its applications in cryopreservation.
Principles of Ice-Free Cryopreservation by Vitrification.
- 2021
Medicine
Vitrification has the potential to enable the superior and convenient cryopreservation of a wide range of biological systems, and it is also increasingly recognized as a successful strategy for surviving harsh environmental conditions in nature.
69 References
Quantitative Estimation of Cryopreservation Viability in Rat Fetal Hippocampal Cells
- 1994
Biology, Medicine
Though cryopreservation of fetal neural tissue for transplantation offers several practical advantages over immediate use, significant additional tissue may be required in comparison to fresh tissue to achieve a suitable quantity of live cells for grafting.
Cryopreservation of precision-cut tissue slices for application in drug metabolism research.
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Medicine
Freezing of neural tissues and their transplantation in the brain of rats: technical details and histological observations
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Medicine, Biology
Long-term morphofunctional survival of guinea pig hippocampal slices after brief treatment with cyclooxygenase inhibitors
- 2006
Biology, Medicine
The aim of the present work was to compare the structural (light microscopy) and functional (PS recording) characteristics of hippocampus slices in standard conditions and after t reatment with the CO inhibitors indomethacin and aspirin during long-term maintenance of slices using hypothermic storage during the night.
Cryopreservation of fetal rat brain tissue later used for intracerebral transplantation.
- 1987
Biology, Medicine
Intracephalic transplants of freeze‐Stored rat hippocampal tissue
- 1986
Biology, Medicine
The survival and cellular and connective organization of intracephalic transplants of developing, freeze‐stored rat hippocampal tissue were examined and their interaction with the host brains were analyzed.
Intraocular grafts of fresh and freeze‐stored rat hippocampal tissue: A comparison of survivability and histological and connective organization
- 1984
Biology, Medicine
Intraocular grafts of rat hippocampal tissue, grafted either directly from the immature donor brains (fresh) or after storage in liquid nitrogen at –196°C (freeze‐stored), were compared with regard…