Poly(A)- and Primer-Independent RNA Polymerase of Norovirus

@article{Fukushi2004PolyAAP,
  title={Poly(A)- and Primer-Independent RNA Polymerase of Norovirus},
  author={Shuetsu Fukushi and Shigeyuki Kojima and Reiko Takai and Fuminori B. Hoshino and Tomoichiro Oka and Naokazu Takeda and Kazuhiko Katayama and Tsutomu Kageyama},
  journal={Journal of Virology},
  year={2004},
  volume={78},
  pages={3889 - 3896},
  url={https://api.semanticscholar.org/CorpusID:35083680}
}
The active recombinant NV RdRp will be of benefit to studies of NV replication and will facilitate the development of specific inhibitors of NV proliferation.
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53 Citations

Characterization of norovirus 3Dpol RNA-dependent RNA polymerase activity and initiation of RNA synthesis.

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Protein-Primed and De Novo Initiation of RNA Synthesis by Norovirus 3Dpol

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Structure(s), function(s), and inhibition of the RNA-dependent RNA polymerase of noroviruses

Characterization of Norovirus RNA replicase for in vitro amplification of RNA

NV3Dpol can be used as an RNA replicase in in vitro RNA + protein evolution with the RNA of special terminal sequences and shows a weak activity of elongation-reaction from a long primer.

Structural and Functional Characterization of Sapovirus RNA-Dependent RNA Polymerase

The first extensive characterization of the key enzyme of replication, the RNA-dependent RNA polymerase (RdRp) associated with the 3Dpol-like protein is reported here on, suggesting structural and functional homologies in the RNAs of human pathogenic calicivirus infection in humans.

The 3′ end of the human norovirus antigenomic sequence is required for its genomic RNA synthesis by RNA-dependent RNA polymerase

It is concluded that the predicted stem-loop structure in the 31 nt and region close to the antisense viral genomic stem sequences are important for initiating the positive-sense human norovirus genomic RNA synthesis by its RdRp.

Murine norovirus-1 3Dpol exhibits RNA-dependent RNA polymerase activity and nucleotidylylates on Tyr of the VPg.

In vitro-transcribed (-) subgenomic (SG) and (+)SG RNA enhanced VPg guanylylation by 9.2 and 3.2 times, respectively, suggesting that the MNV-1 ORF3 region of negative-strand RNA contains a cis-acting element that stimulates 3D(pol)-mediatedVPg guanlylation.

Calicivirus RNA-Dependent RNA Polymerases: Evolution, Structure, Protein Dynamics, and Function

The RdRps of RHDV and related lagoviruses possess the ability to expose a hydrophobic motif, to rearrange Golgi membranes, and to copy RNA at unusually high temperatures, and this review is focused on the structural dynamics, biochemical properties, kinetics, and putative interaction partners of these RdRPS.

Identification of amino acids within norovirus polymerase involved in RNA binding and viral replication.

To identify amino acids involved in RNA synthesis by the viral RNA-dependent RNA polymerase (NS7), NS7 mutants were constructed in which basic amino acids surrounding the catalytic site were substituted with alanine and Electrophoretic mobility shift assay revealed that these residues are important for RNA binding.
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29 References

De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus

It is suggested that HCV NS5B is able to initiate RNA synthesis de novo, and modification of the RNA template by the addition of the chain terminator cordycepin at the 3′ end did not affect synthesis ofThe RNA monomer but eliminated synthesis of the self-priming hairpin dimer RNA.

Expression of hepatitis C virus NS5B protein: Characterization of its RNA polymerase activity and RNA binding

Deletion mutant analysis indicated that the N‐terminal region of NS5B protein was critical for the RNA binding, and this data provide not only important clues for understanding the mechanism of HCV replication, but also a new target of antiviral therapy.

The RNA-dependent RNA polymerases of different members of the family Flaviviridae exhibit similar properties in vitro.

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ABSTRACT The rabbit hemorrhagic disease virus (RHDV) (isolate AST/89) RNA-dependent RNA-polymerase (3Dpol) coding region was expressed in Escherichia coli by using a glutathioneS-transferase-based…

Terminal Nucleotidyl Transferase Activity of RecombinantFlaviviridae RNA-Dependent RNA Polymerases: Implication for Viral RNA Synthesis

Evidence is presented that TNTase activity is an inherent function of HCV and bovine viral diarrhea virus RdRps highly purified from both prokaryotic and eukaryotic cells.

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Chimeric molecules composed of DNA and RNA demonstrated that a DNA molecule containing a 3′-terminal ribocytidylate was able to direct RNA synthesis as efficiently as a sequence composed entirely of RNA.

Gliotoxin: inhibitor of poliovirus RNA synthesis that blocks the viral RNA polymerase 3Dpol

Results indicate that this toxin acts on the poliovirus polymerase 3Dpol, providing the first description of an inhibitor of this viral enzyme.

Identification and properties of the RNA‐dependent RNA polymerase of hepatitis C virus.

Experimental evidence that an RNA‐dependent RNA polymerase is encoded by HCV and that this enzymatic activity is the function of the 65 kDa non‐structural protein 5B (NS5B) is presented, representing a first important step towards a better understanding of the life cycle of the HCV.

Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase

Biochemical analysis of Pro-Pol showed that this enzyme has properties expected of a replicative polymerase, suggesting that pro-Pol is an active form of the FCV RdRP.