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Chien-Jung Huang, Yi-Chen Sun, Karen Christopher, Amy Shih-I Pai, Chia-Ju Lu, Fung-Rong Hu, Szu-Yuan Lin, Wei-Li Chen
Comparison of corneal epitheliotrophic capacities among human platelet lysates and other blood derivatives
www.plosone.org
www.plosone.org
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(and)Tj
1.7291 0 Td
(10%\))Tj
2.2676 0 Td
(of)Tj
1.0148 0 Td
(blood)Tj
2.5172 0 Td
(derivatives)Tj
4.5014 0 Td
(\(HPLs,)Tj
3.0103 0 Td
(HPS,)Tj
2.2507 0 Td
(FBS\))Tj
2.143 0 Td
(for)Tj
1.3833 0 Td
(3)Tj
0.6973 0 Td
(days.)Tj
2.2224 0 Td
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2.2847 0 Td
(incubating)Tj
4.507 0 Td
(for)Tj
1.3833 0 Td
(24,)Tj
-35.6084 -1.2982 Td
(48,)Tj
1.3946 0 Td
(and)Tj
1.7291 0 Td
(72)Tj
1.1735 0 Td
(hours,)Tj
2.7382 0 Td
(the)Tj
1.4683 0 Td
(numbers)Tj
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(of)Tj
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(assay)Tj
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(\(Promega)Tj
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2.4888 0 Td
(Madison,)Tj
3.9853 0 Td
(WI\).)Tj
2.0863 0 Td
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4.4391 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(manufacturer's)Tj
6.2646 0 Td
(instructions,)Tj
5.227 0 Td
(the)Tj
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(system)Tj
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(reduction)Tj
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(a)Tj
0.6519 0 Td
(3-\(4,5-dimethylthiazol-2-)Tj
10.131 0 Td
(yl\)-5-\(3-carboxy-methoxyphen)Tj
12.41 0 Td
(yl\)-2-\(4-sulfophe-)Tj
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(\(MTS\))Tj
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(formazan)Tj
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1.4684 0 Td
(mitochondria)Tj
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(cells.)Tj
2.143 0 Td
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(the)Tj
1.474 0 Td
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1.0148 0 Td
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1.4683 0 Td
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1.0148 0 Td
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1.0148 0 Td
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1.4684 0 Td
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(proliferative)Tj
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(ability.)Tj
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1.6497 0 Td
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1.5873 0 Td
(\(test)Tj
1.9389 0 Td
(wavelength\))Tj
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(and)Tj
1.7292 0 Td
(650nm)Tj
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4.2576 0 Td
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1.2018 0 Td
(ELISA)Tj
2.829 0 Td
(microplate)Tj
4.524 0 Td
(reader)Tj
2.7722 0 Td
(\(Model)Tj
3.1578 0 Td
(ELx)Tj
1.7915 0 Td
(800;)Tj
1.8765 0 Td
(Bio-TEK)Tj
3.7757 0 Td
(instruments.)Tj
5.2724 0 Td
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1.9786 0 Td
(Winooski,)Tj
4.3653 0 Td
(VT\).)Tj
-33.5448 -1.2983 Td
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4.5296 0 Td
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(experiments)Tj
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([)Tj
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(29)Tj
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(])Tj
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1 j
1 J
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11.9999 0 0 11.9999 200.0125 426.2173 cm
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(differentiation-morphology)Tj
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1 TL
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(x)Tj
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BT
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1 TL
-0.0053 Tc
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(5)Tj
ET
Q
q
1 j
1 J
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BT
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1 TL
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(24-well)Tj
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(plastic)Tj
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(cell)Tj
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(FBS\).)Tj
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(washing)Tj
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4.3937 0 Td
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(7.4\))Tj
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(120)Tj
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(tetroxide)Tj
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(60)Tj
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3.4469 0 Td
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(sputter)Tj
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(\(JSM6510LV;)Tj
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(JEOL,)Tj
2.6306 0 Td
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(Tokyo,)Tj
3.0218 0 Td
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(independent)Tj
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(6)Tj
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Q
q
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1 J
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11.9999 0 0 11.9999 200.0125 276.0944 cm
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(differentiation-func)Tj
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Q
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(A)Tj
0.9014 0 Td
(total)Tj
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(1)Tj
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[()]TJ
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(10)Tj
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Q
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1 J
0 w
7.4999 0 0 6.6659 263.5653 249.5622 cm
BT
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1 TL
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(5)Tj
ET
Q
q
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BT
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(incubated)Tj
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(\(HPLs,)Tj
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(and)Tj
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(FBS\))Tj
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(\(Corning)Tj
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3.0841 0 Td
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(MA\))Tj
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(0.4)Tj
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(MA\))Tj
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(0\))Tj
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(3)Tj
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(\(day)Tj
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(3\))Tj
1.0375 0 Td
(when)Tj
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(the)Tj
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[()]TJ
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(supporting)Tj
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(membrane)Tj
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(filter)Tj
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(consis-)Tj
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(29)Tj
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(].)Tj
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1 J
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BT
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(model)Tj
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4.1008 0 Td
(wound)Tj
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(animals)Tj
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(guidelines)Tj
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(ARVO)Tj
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(Vision)Tj
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(Research.)Tj
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1.7858 0 Td
(protocol)Tj
3.5943 0 Td
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1.7008 0 Td
(approved)Tj
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(the)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
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