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Katarzyna Kryściak, Joanna Majerczak, Jakub Kryściak, Dawid Łochyński, Dominik Kaczmarek, Hanna Drzymała-Celichowska, Piotr Krutki, Anna Gawedzka, Magdalena Guzik, Michał Korostynski, Zbigniew Szkutnik, Elżbieta Pyza, Wiesława Jarmuszkiewicz, Jerzy A. Zoladz, Jan Celichowski
Adaptation of motor unit contractile properties in rat medial gastrocnemius to treadmill endurance training: Relationship to muscle mitochondrial biogenesis
www.plosone.org
www.plosone.org
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(0.5)Tj
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-36.0961 -1.2983 Td
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2.1203 0 Td
(measured)Tj
4.1102 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(muscle)Tj
-33.4032 -1.2982 Td
(samples.)Tj
3.6056 0 Td
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3.0104 0 Td
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(homogenized)Tj
5.6522 0 Td
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1.0318 0 Td
(1)Tj
0.6973 0 Td
(ml)Tj
1.287 0 Td
(Trizol)Tj
2.6362 0 Td
(reagent)Tj
3.1804 0 Td
(\(Invitrogen,)Tj
5.0116 0 Td
(Carls-)Tj
-34.0778 -1.2983 Td
(bad,)Tj
1.9105 0 Td
(CA,)Tj
1.7971 0 Td
(USA\),)Tj
2.6816 0 Td
(and)Tj
1.7291 0 Td
(total)Tj
2.0013 0 Td
(RNA)Tj
2.262 0 Td
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(extracted)Tj
3.8834 0 Td
(according)Tj
4.1896 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(manufacturer's)Tj
6.2645 0 Td
(protocol.)Tj
3.8211 0 Td
(The)Tj
-34.7355 -1.2983 Td
(RNA)Tj
2.262 0 Td
(concentration)Tj
5.8166 0 Td
(was)Tj
1.7008 0 Td
(measured)Tj
4.1102 0 Td
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(a)Tj
0.6519 0 Td
(ND-1000)Tj
3.9458 0 Td
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5.5729 0 Td
(\(NanoDrop)Tj
4.9209 0 Td
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-31.3679 -1.3039 Td
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1.9842 0 Td
(Montchanin,)Tj
5.4368 0 Td
(DE,)Tj
1.7518 0 Td
(USA\).)Tj
2.6816 0 Td
(The)Tj
1.7858 0 Td
(quality)Tj
2.9537 0 Td
(of)Tj
1.0148 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.7008 0 Td
(determined)Tj
4.8472 0 Td
(using)Tj
2.3924 0 Td
(an)Tj
1.2019 0 Td
(RNA)Tj
2.262 0 Td
(6000)Tj
2.1203 0 Td
(Nano)Tj
-34.3953 -1.2982 Td
(LabChip)Tj
3.6736 0 Td
(Kit)Tj
1.457 0 Td
(\(Agilent,)Tj
3.7191 0 Td
(Palo)Tj
1.9672 0 Td
(Alto,)Tj
2.1827 0 Td
(CA,)Tj
1.7914 0 Td
(USA\))Tj
2.4548 0 Td
(and)Tj
1.7291 0 Td
(measured)Tj
4.1103 0 Td
(using)Tj
2.3924 0 Td
(an)Tj
1.2019 0 Td
(Agilent)Tj
3.1521 0 Td
(Bioanalyzer)Tj
-29.8316 -1.2983 Td
(2100)Tj
2.1203 0 Td
(\(Agilent,)Tj
3.719 0 Td
(Palo)Tj
1.9672 0 Td
(Alto,)Tj
2.1827 0 Td
(CA,)Tj
1.7915 0 Td
(USA\).)Tj
2.6815 0 Td
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3.2995 0 Td
(transcription)Tj
5.4312 0 Td
(was)Tj
1.7008 0 Td
(performed)Tj
4.4673 0 Td
(with)Tj
1.9956 0 Td
(Omniscript)Tj
-31.3566 -1.3039 Td
(reverse)Tj
3.05 0 Td
(transcriptase)Tj
5.3291 0 Td
(enzyme)Tj
3.2995 0 Td
(\(Qiagen)Tj
3.4469 0 Td
(Inc.,)Tj
1.9843 0 Td
(Valencia,)Tj
3.9174 0 Td
(CA,)Tj
1.7915 0 Td
(USA\))Tj
2.4548 0 Td
(at)Tj
0.9524 0 Td
(37C)Tj
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(for)Tj
1.3833 0 Td
(60)Tj
1.1736 0 Td
(minutes.)Tj
-31.0108 -1.2983 Td
(The)Tj
1.7801 0 Td
(qPCR)Tj
2.5738 0 Td
(reactions)Tj
3.8495 0 Td
(were)Tj
2.1259 0 Td
(performed)Tj
4.4674 0 Td
(using)Tj
2.3924 0 Td
(Assay-On-Demand)Tj
7.9994 0 Td
(TaqMan)Tj
3.6453 0 Td
(probes)Tj
2.9083 0 Td
(\()Tj
/F13 1 Tf
[()47(\r)3()]TJ
/F9 1 Tf
(,)Tj
-31.7421 -1.2983 Td
(Rn01488777_g1;)Tj
/F13 1 Tf
6.8541 0 Td
[()40()2( )]TJ
/F9 1 Tf
(,)Tj
3.2938 0 Td
(Rn00568762_m1;)Tj
/F13 1 Tf
7.2113 0 Td
[()-11()3()11()14()]TJ
/F9 1 Tf
(,)Tj
2.6249 0 Td
(Rn00574541_m1;)Tj
/F13 1 Tf
7.2113 0 Td
[(\n)-6()4()]TJ
/F9 1 Tf
(,)Tj
2.5681 0 Td
(Rn00592200_m1;)Tj
/F13 1 Tf
-29.7635 -1.3039 Td
[()-8()]TJ
/F9 1 Tf
(,)Tj
2.5738 0 Td
(Rn00594496_m1)Tj
6.9902 0 Td
(and)Tj
/F13 1 Tf
1.7291 0 Td
[()-8( )]TJ
/F9 1 Tf
(,)Tj
2.5795 0 Td
(Rn00500120_m1\))Tj
7.3247 0 Td
(according)Tj
4.1896 0 Td
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1.0261 0 Td
(the)Tj
1.4683 0 Td
(manufacturer's)Tj
6.2646 0 Td
(proto-)Tj
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1.6214 0 Td
(\(Thermo)Tj
3.821 0 Td
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4.1272 0 Td
(Paisley,)Tj
3.1918 0 Td
(UK\))Tj
1.9672 0 Td
(and)Tj
1.7292 0 Td
(were)Tj
2.1259 0 Td
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1.6668 0 Td
(on)Tj
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(a)Tj
0.6519 0 Td
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3.0161 0 Td
(Real-Time)Tj
4.4163 0 Td
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(\(Bio-Rad,)Tj
4.0705 0 Td
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2.7099 0 Td
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2.1316 0 Td
(CA,)Tj
1.7915 0 Td
(USA\).)Tj
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6.2248 0 Td
(50)Tj
1.1735 0 Td
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1.0148 0 Td
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2.2054 0 Td
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(an)Tj
1.2018 0 Td
(individual)Tj
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(animal\))Tj
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1.6952 0 Td
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(reaction.)Tj
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1.0148 0 Td
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(gene)Tj
2.0806 0 Td
(hypoxanthine)Tj
5.7373 0 Td
(phosphoribosyltransfera)Tj
9.7114 0 Td
(se)Tj
1.0035 0 Td
(1)Tj
0.6973 0 Td
(\()Tj
/F13 1 Tf
[()33()11()-19()]TJ
/F9 1 Tf
(,)Tj
3.1181 0 Td
(Rn01527840_m1\))Tj
7.3304 0 Td
(was)Tj
-33.596 -1.2982 Td
(quantified)Tj
4.3086 0 Td
(to)Tj
1.0261 0 Td
(control)Tj
3.1181 0 Td
(for)Tj
1.389 0 Td
(variation)Tj
3.7984 0 Td
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(cDNA)Tj
2.8006 0 Td
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3.9401 0 Td
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1.7858 0 Td
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1.0148 0 Td
(RNA)Tj
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-32.7739 -1.2983 Td
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1.0147 0 Td
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 214.9228 490.8472 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
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(-\(threshold)Tj
4.7547 0 Td
(cycle\))Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 267.874 486.4251 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 457.6818 cm
BT
/F16 1 Tf
1 TL
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6.274 0 Td
(DNA)Tj
2.4189 0 Td
(\(mtDNA\))Tj
4.2946 0 Td
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ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 440.674 cm
BT
/F9 1 Tf
1 TL
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(was)Tj
1.7007 0 Td
(isolated)Tj
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(\(MGF)Tj
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(MGS,)Tj
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(separately\))Tj
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(Mini)Tj
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(USA\))Tj
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(according)Tj
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1.4683 0 Td
(manufacturer's)Tj
6.2645 0 Td
(protocol,)Tj
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(and)Tj
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(quantified)Tj
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(by)Tj
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(using)Tj
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(a)Tj
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(ND-1000)Tj
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(Spectrometer)Tj
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(\(NanoDrop)Tj
4.9209 0 Td
(Technologies,)Tj
-29.9053 -1.2982 Td
(Inc.\).)Tj
2.3243 0 Td
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1.7802 0 Td
(mtDNA)Tj
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(content)Tj
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(was)Tj
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(measured)Tj
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(using)Tj
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(qPCR)Tj
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(with)Tj
1.9956 0 Td
(a)Tj
0.6576 0 Td
(CFX96)Tj
3.0161 0 Td
(Real-Time)Tj
4.4163 0 Td
(system)Tj
2.9367 0 Td
(\(Bio-)Tj
-34.6561 -1.304 Td
(Rad\))Tj
2.1429 0 Td
([)Tj
0.83 0.64 0.02 0 k
(54)Tj
0 g
(].)Tj
2.075 0 Td
(TaqMan)Tj
3.6453 0 Td
(assay)Tj
2.2677 0 Td
(\(Thermo)Tj
3.8211 0 Td
(Fisher)Tj
2.6986 0 Td
(Scientific\))Tj
4.2406 0 Td
(targeting)Tj
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3.7927 0 Td
[()40()2()]TJ
/F9 1 Tf
2.109 0 Td
(mitochondrial)Tj
5.9697 0 Td
(gene)Tj
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(was)Tj
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(used)Tj
2.0579 0 Td
(to)Tj
1.0261 0 Td
(quantify)Tj
3.5433 0 Td
(the)Tj
1.474 0 Td
(mtDNA.)Tj
3.719 0 Td
(The)Tj
1.7802 0 Td
(primers)Tj
3.3505 0 Td
(and)Tj
1.7291 0 Td
(probe)Tj
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(for)Tj
1.3833 0 Td
(qPCR)Tj
2.5739 0 Td
(analysis)Tj
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(of)Tj
1.0148 0 Td
(mtDNA)Tj
3.498 0 Td
(were)Tj
-33.0234 -1.2983 Td
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3.7643 0 Td
(using)Tj
2.3868 0 Td
(File)Tj
1.6838 0 Td
(Builder)Tj
3.1634 0 Td
(3.1,)Tj
1.6271 0 Td
(and)Tj
1.7291 0 Td
(ordered)Tj
3.3732 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.652 0 Td
(custom)Tj
3.1577 0 Td
(TaqMan)Tj
3.6454 0 Td
(assay)Tj
2.2677 0 Td
(\(Thermo)Tj
3.8154 0 Td
(Fisher)Tj
2.6986 0 Td
(Scien-)Tj
-34.9793 -1.3039 Td
(tific\).)Tj
2.33 0 Td
(The)Tj
1.7802 0 Td
(TaqMan)Tj
3.6453 0 Td
(probe)Tj
2.5455 0 Td
(\(assay)Tj
2.6078 0 Td
(ID)Tj
1.2983 0 Td
(CCX00SS,)Tj
4.3256 0 Td
(MT-ATP6\))Tj
4.7452 0 Td
(was)Tj
1.6951 0 Td
(labeled)Tj
3.0331 0 Td
(with)Tj
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(the)Tj
1.4683 0 Td
(FAM)Tj
2.3244 0 Td
(fluores-)Tj
-33.7944 -1.2983 Td
(cent)Tj
1.9105 0 Td
(reporter.)Tj
3.7304 0 Td
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1.7858 0 Td
(quantity)Tj
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(mtDNA)Tj
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(corrected)Tj
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(by)Tj
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(simultaneous)Tj
5.5445 0 Td
(measurement)Tj
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(of)Tj
1.0148 0 Td
(the)Tj
-34.5881 -1.2982 Td
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(nuclear)Tj
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3.1918 0 Td
[()97()2()-19()]TJ
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1.8652 0 Td
(gene.)Tj
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(custom)Tj
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(TaqMan)Tj
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(assay)Tj
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(was)Tj
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(quantify)Tj
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(the)Tj
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(nuclear)Tj
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(DNA)Tj
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(\(nDNA\))Tj
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(\(Custom)Tj
3.736 0 Td
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Q
q
1 j
1 J
0 w
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BT
/F17 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
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BT
/F9 1 Tf
1 TL
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(rat_Tert_CCLJI3S;)Tj
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(Scien-)Tj
-34.3556 -1.2982 Td
(tific\).)Tj
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(20-)Tj
/F15 1 Tf
[()]TJ
/F9 1 Tf
(l)Tj
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(PCR)Tj
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(reaction)Tj
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(contained)Tj
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(10)Tj
/F15 1 Tf
1.1735 0 Td
[()]TJ
/F9 1 Tf
(l)Tj
0.9694 0 Td
(TaqMan)Tj
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(Universal)Tj
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(PCR)Tj
2.0636 0 Td
(Master)Tj
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(\(Thermo)Tj
-32.9043 -1.2983 Td
(Fisher)Tj
2.6986 0 Td
(Scientific\),)Tj
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(1)Tj
/F15 1 Tf
0.6973 0 Td
[()]TJ
/F9 1 Tf
(l)Tj
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(of)Tj
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(TaqMan)Tj
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(primers)Tj
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(and)Tj
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(probe)Tj
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(\(20X\))Tj
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(mix,)Tj
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(and)Tj
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(510)Tj
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(conditions)Tj
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(min)Tj
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(at)Tj
0.9525 0 Td
(50C)Tj
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(then)Tj
2.0126 0 Td
(10)Tj
1.1735 0 Td
(min)Tj
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(followed)Tj
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(40)Tj
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(cycles)Tj
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(con-)Tj
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(of)Tj
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(15)Tj
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(s)Tj
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(of)Tj
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(denaturation)Tj
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(at)Tj
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(95C)Tj
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(and)Tj
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(60)Tj
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(annealing/extension)Tj
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(60C.)Tj
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(and)Tj
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(nDNA)Tj
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(copy)Tj
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(which)Tj
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(based)Tj
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(on)Tj
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(the)Tj
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(lin-)Tj
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1.4457 0 Td
(relationship)Tj
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(between)Tj
3.5319 0 Td
(the)Tj
1.4684 0 Td
(crossing)Tj
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(point)Tj
2.3584 0 Td
(cycle)Tj
2.1883 0 Td
(values)Tj
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(and)Tj
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(the)Tj
1.4684 0 Td
(logarithm)Tj
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(of)Tj
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(the)Tj
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(starting)Tj
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(copy)Tj
-35.2797 -1.3039 Td
(number.)Tj
ET
Q
q
1 j
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