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Julia Uebele, Christoph Stein, Minh-Thu Nguyen, Anja Schneider, Franziska Kleinert, Olga Tichá, Gabriele Bierbaum, Friedrich Götz, Isabelle Bekeredjian-Ding
Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus
www.plospathogens.org
www.plospathogens.org
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(27)Tj
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(28)Tj
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[()10()4()]TJ
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(29)Tj
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(],)Tj
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(and)Tj
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0.9241 0 Td
[()6(\r)7( )]TJ
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(\(PBP2a\),)Tj
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(30)Tj
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[()6(\r)7( )]TJ
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1.0998 0 Td
[()10()14()]TJ
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(CD8)Tj
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q
1 j
1 J
0 w
7.4999 0 0 6.6659 333.1842 633.8267 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 339.7606 629.4047 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
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(or)Tj
1.0941 0 Td
(CD4)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 369.4677 633.8267 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 376.044 629.4047 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
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(T)Tj
0.8277 0 Td
(cells.)Tj
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(\r)Tj
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(cells)Tj
1.9219 0 Td
(were)Tj
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4.0818 0 Td
(incubation)Tj
4.5524 0 Td
(\()Tj
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(S1A)Tj
2.194 0 Td
(Fig)Tj
0 g
(\).)Tj
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(pT7CFE1-cMyc)Tj
6.6047 0 Td
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(subse-)Tj
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3.2258 0 Td
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3.4186 0 Td
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1.0261 0 Td
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5.0286 0 Td
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3.1238 0 Td
(\(non-coding,)Tj
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(NC\))Tj
1.9616 0 Td
(\()Tj
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(S1A)Tj
2.194 0 Td
(Fig)Tj
0 g
(\).)Tj
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4.8018 0 Td
(\(LF\))Tj
1.9673 0 Td
(alone)Tj
-35.2854 -1.2983 Td
(did)Tj
1.542 0 Td
(not)Tj
1.5704 0 Td
(induce)Tj
2.9424 0 Td
(IFN)Tj
/F10 1 Tf
(\r)Tj
/F9 1 Tf
2.279 0 Td
(production)Tj
4.7112 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(S1A)Tj
2.1996 0 Td
(Fig)Tj
0 g
(\).)Tj
2.0466 0 Td
(As)Tj
1.2642 0 Td
(expected)Tj
3.719 0 Td
(due)Tj
1.7008 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(method)Tj
3.3221 0 Td
(inherent)Tj
3.6284 0 Td
(predomi-)Tj
-33.4202 -1.3039 Td
(nance)Tj
2.5965 0 Td
(of)Tj
1.0148 0 Td
(MHCI-restricted)Tj
7.0016 0 Td
(peptide)Tj
3.1917 0 Td
(presentation)Tj
5.2213 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(])Tj
1.8482 0 Td
(the)Tj
1.474 0 Td
(number)Tj
3.4129 0 Td
(of)Tj
1.0148 0 Td
(IFN)Tj
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(\r)Tj
/F9 1 Tf
(-secreting)Tj
6.2135 0 Td
(cells)Tj
1.9219 0 Td
(was)Tj
-34.9112 -1.2982 Td
(higher)Tj
2.8006 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(CD8)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 271.8425 555.8173 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 278.3622 551.3952 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(than)Tj
2.0295 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4684 0 Td
(CD4)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 342.4818 555.8173 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 349.0015 551.3952 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
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(T)Tj
0.8333 0 Td
(cell)Tj
1.5591 0 Td
(fraction)Tj
3.3562 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(S1A)Tj
2.194 0 Td
(Fig)Tj
0 g
(\).)Tj
2.0466 0 Td
(Furthermore,)Tj
5.6125 0 Td
(the)Tj
1.474 0 Td
(number)Tj
3.4129 0 Td
(of)Tj
-35.3874 -1.2982 Td
(IFN)Tj
/F10 1 Tf
(\r)Tj
/F9 1 Tf
(-secreting)Tj
6.2079 0 Td
(T)Tj
0.8334 0 Td
(cells)Tj
1.9218 0 Td
(could)Tj
2.4492 0 Td
(be)Tj
1.1508 0 Td
(enriched)Tj
3.736 0 Td
(by)Tj
1.1792 0 Td
(isolating)Tj
3.6169 0 Td
(CD45RO)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 448.1574 542.8346 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 454.6771 538.4125 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(T)Tj
0.8333 0 Td
(cells,)Tj
2.143 0 Td
(indicating)Tj
4.269 0 Td
(that)Tj
1.7744 0 Td
(IFN)Tj
/F10 1 Tf
(\r)Tj
/F9 1 Tf
2.2791 0 Td
(is)Tj
-36.765 -1.3039 Td
(released)Tj
3.4299 0 Td
(from)Tj
2.2053 0 Td
(pre-existent)Tj
4.9663 0 Td
(memory)Tj
3.6169 0 Td
(T)Tj
0.8334 0 Td
(cells)Tj
1.9219 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(S1B)Tj
2.0863 0 Td
(Fig)Tj
0 g
(\).)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 490.6771 cm
BT
/F0 1 Tf
1 TL
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0 0 Td
(mRNA-encoded)Tj
7.2567 0 Td
(antigen)Tj
3.4536 0 Td
(supersedes)Tj
5.3291 0 Td
(protein)Tj
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(antigen)Tj
3.4536 0 Td
(in)Tj
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(the)Tj
1.5922 0 Td
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[()]TJ
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2.3197 0 Td
(production)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F9 1 Tf
1 TL
-0.004 Tc
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1.3379 0 Td
(exclude)Tj
3.2541 0 Td
(immune)Tj
3.6284 0 Td
(effects)Tj
2.7269 0 Td
(owing)Tj
2.6929 0 Td
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1.0204 0 Td
(the)Tj
1.4741 0 Td
(ivT)Tj
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(mRNA)Tj
3.0784 0 Td
(technique)Tj
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(we,)Tj
1.5533 0 Td
(next,)Tj
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4.2463 0 Td
(the)Tj
1.4683 0 Td
(T)Tj
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(cell)Tj
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4.8075 0 Td
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3.4696 0 Td
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1.0148 0 Td
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7.1432 0 Td
(antigens)Tj
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1.0261 0 Td
(that)Tj
1.7802 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
-35.6596 -1.2982 Td
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3.1521 0 Td
(antigens.)Tj
3.7927 0 Td
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(this)Tj
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(end,)Tj
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1.3266 0 Td
(delivered)Tj
3.8834 0 Td
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(to)Tj
1.0261 0 Td
(MoDC,)Tj
3.2428 0 Td
(co-incubated)Tj
-30.6479 -1.2983 Td
(these)Tj
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(with)Tj
2.0013 0 Td
(CD4)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 261.2409 425.1401 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F9 1 Tf
1 TL
-0.004 Tc
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(or)Tj
1.0998 0 Td
(CD8)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 297.5244 425.1401 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
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BT
/F9 1 Tf
1 TL
-0.004 Tc
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(T)Tj
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(cell)Tj
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3.719 0 Td
(and)Tj
1.7292 0 Td
(quantified)Tj
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(IFN)Tj
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(\r)Tj
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2.279 0 Td
(secretion)Tj
3.8438 0 Td
(by)Tj
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-34.1458 -1.3039 Td
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(\(TT\))Tj
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(pool)Tj
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(from)Tj
2.2053 0 Td
(the)Tj
1.4683 0 Td
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3.9912 0 Td
(H1N1)Tj
2.6759 0 Td
(matrix)Tj
2.8743 0 Td
(protein)Tj
3.1521 0 Td
(1)Tj
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(\(MP1\))Tj
2.829 0 Td
(were)Tj
2.1203 0 Td
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-36.5666 -1.2982 Td
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(antigen)Tj
3.2031 0 Td
(controls)Tj
3.4866 0 Td
(for)Tj
1.3832 0 Td
(CD4)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 323.1496 399.1181 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(and)Tj
1.7291 0 Td
(CD8)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 365.7259 399.1181 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
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(+)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F9 1 Tf
1 TL
-0.004 Tc
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(T)Tj
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(cell)Tj
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(of)Tj
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(the)Tj
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(IFN)Tj
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(\r)Tj
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2.279 0 Td
(secretion)Tj
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4.1838 0 Td
(higher)Tj
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(CD8)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 434.3811 386.1354 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
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BT
/F9 1 Tf
1 TL
-0.004 Tc
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(T)Tj
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(cells)Tj
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Q
q
1 j
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7.4999 0 0 6.6659 517.833 386.1354 cm
BT
/F9 1 Tf
1 TL
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(+)Tj
ET
Q
q
1 j
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BT
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1 TL
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(T)Tj
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(cells)Tj
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(Fig)Tj
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(1)Tj
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(\).)Tj
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(antigens)Tj
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(mRNA,)Tj
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1.9672 0 Td
[()10()4()]TJ
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(\r)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 454.337 360.17 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(and)Tj
1.7291 0 Td
(CD8)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 496.97 360.17 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 503.4897 355.6913 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
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