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Ludmila A. Alekseeva, Nadezhda L. Mironova, Evgenyi V. Brenner, Alexander M. Kurilshikov, Olga A. Patutina, Marina A. Zenkova
Alteration of the exDNA profile in blood serum of LLC-bearing mice under the decrease of tumour invasion potential by bovine pancreatic DNase I treatment
www.plosone.org
www.plosone.org
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1.1792 0 Td
(libitum.)Tj
3.3789 0 Td
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1.4003 0 Td
(animal)Tj
2.965 0 Td
(procedures)Tj
-32.6435 -1.3039 Td
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(carried)Tj
3.0387 0 Td
(out)Tj
1.5533 0 Td
(in)Tj
1.0375 0 Td
(strict)Tj
2.2394 0 Td
(accordance)Tj
4.7338 0 Td
(with)Tj
2.0013 0 Td
(the)Tj
1.4683 0 Td
(approved)Tj
3.9741 0 Td
(protocol)Tj
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(and)Tj
1.7292 0 Td
(recommendations)Tj
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2.9253 0 Td
(use)Tj
1.5307 0 Td
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1.7348 0 Td
(care)Tj
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(of)Tj
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(laboratory)Tj
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(animals)Tj
3.3336 0 Td
(\(ECC)Tj
2.4548 0 Td
(Directive)Tj
3.8834 0 Td
(2010/63/EU\).)Tj
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1.7801 0 Td
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1.1793 0 Td
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(on)Tj
1.2755 0 Td
(the)Tj
1.4684 0 Td
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1.4683 0 Td
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(the)Tj
1.474 0 Td
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(narcosis)Tj
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1.7008 0 Td
(20.2)Tj
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1.8708 0 Td
[()]TJ
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0.788 0 Td
(1.5)Tj
1.4004 0 Td
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0.907 0 Td
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1.338 0 Td
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1.491 0 Td
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3.3335 0 Td
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1.9956 0 Td
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1.4683 0 Td
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4.6091 0 Td
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4.388 0 Td
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Q
q
1 j
1 J
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11.9999 0 0 11.9999 200.0125 420.0377 cm
BT
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1 TL
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(transplantation)Tj
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Q
q
1 j
1 J
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BT
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1 TL
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1.0261 0 Td
(14-week-old)Tj
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(male)Tj
2.143 0 Td
(C57Bl/6)Tj
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(\(10)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 232.1007 394.4692 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 237.8834 390.0472 cm
BT
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-0.004 Tc
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(cells/mouse\))Tj
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(suspended)Tj
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(0.1)Tj
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(buffer)Tj
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(the)Tj
1.4683 0 Td
(right)Tj
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(thighs.)Tj
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(4)Tj
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(LLC)Tj
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(10\):)Tj
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(1,)Tj
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(received)Tj
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(i.m.)Tj
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(buffer)Tj
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(i.m.)Tj
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(bovine)Tj
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(pancreatic)Tj
4.3597 0 Td
(DNase)Tj
2.914 0 Td
(I)Tj
0.5612 0 Td
(\(Sigma,)Tj
-34.3272 -1.2983 Td
(USA\))Tj
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(a)Tj
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(dose)Tj
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(0.12)Tj
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(mg/kg)Tj
2.7949 0 Td
(\(0.1)Tj
1.7405 0 Td
(ml\).)Tj
1.8481 0 Td
(DNase)Tj
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(I)Tj
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(daily)Tj
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(except)Tj
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(weekends.)Tj
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(total)Tj
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(of)Tj
1.0148 0 Td
(injections)Tj
4.1216 0 Td
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(ten.)Tj
1.7177 0 Td
(Tumor)Tj
3.0558 0 Td
(size)Tj
1.6837 0 Td
(was)Tj
1.7008 0 Td
(determined)Tj
4.8472 0 Td
(every)Tj
2.3528 0 Td
(other)Tj
2.347 0 Td
(day)Tj
1.6328 0 Td
(using)Tj
-33.3748 -1.3039 Td
(caliper)Tj
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(measurements)Tj
6.032 0 Td
(in)Tj
1.0318 0 Td
(three)Tj
2.2677 0 Td
(perpendicular)Tj
5.8167 0 Td
(dimensions.)Tj
5.0797 0 Td
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3.0557 0 Td
(volumes)Tj
3.5603 0 Td
(were)Tj
2.1203 0 Td
(calculated)Tj
4.2066 0 Td
(as)Tj
-36.0734 -1.2982 Td
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0.9127 0 Td
(=)Tj
0.7994 0 Td
(\()Tj
/F12 1 Tf
[()]TJ
/F8 1 Tf
(/6)Tj
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1.8822 0 Td
[()]TJ
/F8 1 Tf
0.7936 0 Td
(length)Tj
/F12 1 Tf
2.7269 0 Td
[()]TJ
/F8 1 Tf
0.788 0 Td
(width)Tj
/F12 1 Tf
2.5285 0 Td
[()]TJ
/F8 1 Tf
0.788 0 Td
(height\).)Tj
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(day)Tj
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(15)Tj
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(retro-orbital)Tj
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(60)Tj
1.1735 0 Td
(min)Tj
1.8538 0 Td
(after)Tj
2.0353 0 Td
(the)Tj
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(DNase)Tj
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(I)Tj
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(injection.)Tj
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(mice)Tj
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(and)Tj
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(the)Tj
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(and)Tj
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(4%)Tj
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(formaldehyde)Tj
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(metastases)Tj
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(microscope.)Tj
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(ml\))Tj
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(collected)Tj
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(the)Tj
1.4683 0 Td
(retro-orbital)Tj
5.1704 0 Td
(sinus)Tj
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(two)Tj
1.7121 0 Td
(times)Tj
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(an)Tj
1.2019 0 Td
(inter-)Tj
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(7)Tj
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(days.)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 208.0062 cm
BT
/F0 1 Tf
1 TL
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(serum)Tj
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(preparation)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F8 1 Tf
1 TL
-0.004 Tc
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(Blood)Tj
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(serum)Tj
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(LLC)Tj
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(saline)Tj
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(I)Tj
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(by)Tj
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(clot)Tj
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(formation)Tj
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(37C)Tj
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(30)Tj
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(4C)Tj
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(discard)Tj
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(and)Tj
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(centrifugation)Tj
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(\(4000)Tj
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(rpm,)Tj
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(4C,)Tj
1.9729 0 Td
(20)Tj
1.1735 0 Td
(min\))Tj
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(remove)Tj
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(cell)Tj
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(stored)Tj
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[()]TJ
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(70C)Tj
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(until)Tj
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Q
q
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1 J
0 w
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BT
/F0 1 Tf
1 TL
-0.0033 Tc
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(Analysis)Tj
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(of)Tj
1.0441 0 Td
(the)Tj
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(area)Tj
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(of)Tj
1.0441 0 Td
(metastases)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F8 1 Tf
1 TL
-0.004 Tc
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(morphometric)Tj
6.0718 0 Td
(analysis,)Tj
3.549 0 Td
(lungs)Tj
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(were)Tj
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(fixed)Tj
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(in)Tj
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(10%)Tj
1.9275 0 Td
(neutral-buffered)Tj
6.7748 0 Td
(formalin)Tj
3.702 0 Td
(\(pH)Tj
1.8482 0 Td
(7.0\),)Tj
1.9616 0 Td
(rou-)Tj
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(tinely)Tj
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(processed)Tj
4.1386 0 Td
(and)Tj
1.7291 0 Td
(embedded)Tj
4.3994 0 Td
(in)Tj
1.0374 0 Td
(paraffin.)Tj
3.5943 0 Td
(Paraffin)Tj
3.4073 0 Td
(sections)Tj
3.4129 0 Td
(\(5)Tj
/F12 1 Tf
1.0318 0 Td
[()]TJ
/F8 1 Tf
(m\))Tj
1.8878 0 Td
(were)Tj
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(stained)Tj
3.0784 0 Td
(with)Tj
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(haema-)Tj
-34.2933 -1.2982 Td
(toxylin)Tj
3.0047 0 Td
(and)Tj
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(eosin,)Tj
2.5568 0 Td
(microscopically)Tj
6.4856 0 Td
(examined)Tj
4.1329 0 Td
(and)Tj
1.7292 0 Td
(scanned.)Tj
3.7133 0 Td
(Paraffin)Tj
3.4072 0 Td
(sections)Tj
3.4073 0 Td
(\(5)Tj
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1.0374 0 Td
[()]TJ
/F8 1 Tf
(m\))Tj
1.8879 0 Td
(were)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 269.6881 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(The)Tj
1.9134 0 Td
(antimetastatic)Tj
6.2858 0 Td
(action)Tj
2.8418 0 Td
(of)Tj
1.0417 0 Td
(DNase)Tj
3.2245 0 Td
(I)Tj
0.496 0 Td
(is)Tj
0.9284 0 Td
(mediated)Tj
4.2591 0 Td
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