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Dan Ting Kho, Rebecca Johnson, Laverne Robilliard, Elyce du Mez, Julie McIntosh, Simon J. O’Carroll, Catherine E. Angel, E. Scott Graham
ECIS technology reveals that monocytes isolated by CD14+ve selection mediate greater loss of BBB integrity than untouched monocytes, which occurs to a greater extent with IL-1β activated endothelium in comparison to TNFα
www.plosone.org
www.plosone.org
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(hypothesised)Tj
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(that)Tj
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(this)Tj
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(would)Tj
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q
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(\(PFA\))Tj
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1.3832 0 Td
(2)Tj
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(10min.)Tj
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4.0762 0 Td
(and)Tj
1.7348 0 Td
(the)Tj
-36.2208 -1.3039 Td
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4.592 0 Td
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1.9956 0 Td
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1.9956 0 Td
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2.1543 0 Td
(Triton-X)Tj
3.8268 0 Td
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(PBS)Tj
1.8312 0 Td
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1.3832 0 Td
(10)Tj
1.1736 0 Td
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2.5682 0 Td
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1.9275 0 Td
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(5%)Tj
1.4513 0 Td
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-35.2627 -1.2982 Td
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2.4945 0 Td
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(5)Tj
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3.1804 0 Td
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1.9956 0 Td
(PBS)Tj
1.8369 0 Td
(thrice)Tj
2.5284 0 Td
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(1%)Tj
1.457 0 Td
(BSA)Tj
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(in)Tj
-35.7673 -1.304 Td
(PBS)Tj
1.8311 0 Td
(for)Tj
1.389 0 Td
(45)Tj
1.1679 0 Td
(min.)Tj
2.0806 0 Td
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3.1748 0 Td
(three)Tj
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2.0012 0 Td
(PBST)Tj
2.4435 0 Td
(and)Tj
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4.1782 0 Td
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-32.0936 -1.2982 Td
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(555444,)Tj
3.2995 0 Td
(BD)Tj
1.5364 0 Td
(Biosciences,)Tj
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(1:100\),)Tj
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(\(cat#)Tj
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(sc6458,)Tj
3.1351 0 Td
(Santa)Tj
2.4094 0 Td
(Cruz,)Tj
2.4208 0 Td
(1:100\),)Tj
2.914 0 Td
(anti-ZO-1)Tj
-32.9837 -1.2983 Td
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(339100,)Tj
3.2995 0 Td
(Invitrogen,)Tj
4.6715 0 Td
(1:100\))Tj
2.6872 0 Td
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1.0998 0 Td
(actin)Tj
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(\(cat#)Tj
2.194 0 Td
(R37110,)Tj
3.4469 0 Td
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4.2916 0 Td
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3.1748 0 Td
(1:50\))Tj
2.211 0 Td
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1.3833 0 Td
(1)Tj
0.6973 0 Td
(h)Tj
0.7483 0 Td
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-35.2344 -1.2983 Td
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5.3517 0 Td
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2.16 0 Td
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2.126 0 Td
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3.1748 0 Td
(three)Tj
2.2677 0 Td
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1.9956 0 Td
(PBST)Tj
2.4491 0 Td
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1.7291 0 Td
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2.1203 0 Td
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4.1783 0 Td
(in)Tj
1.0375 0 Td
(corresponding)Tj
-30.9768 -1.3039 Td
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2.4717 0 Td
(Fluor)Tj
2.3981 0 Td
(488)Tj
1.6498 0 Td
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4.6318 0 Td
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(antibody)Tj
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(\(Goat)Tj
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(anti-Mouse,)Tj
5.0569 0 Td
(cat#)Tj
1.8482 0 Td
(A-11001,)Tj
3.8608 0 Td
(Invitrogen,)Tj
-32.4678 -1.2982 Td
(1:400;)Tj
2.5738 0 Td
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2.1713 0 Td
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4.9776 0 Td
(cat#)Tj
1.8539 0 Td
(A-11008,)Tj
3.8607 0 Td
(Invitrogen,)Tj
4.6658 0 Td
(1:400\))Tj
2.6873 0 Td
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1.7347 0 Td
(nuclei)Tj
2.6533 0 Td
(stained)Tj
3.0784 0 Td
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(Hoescht)Tj
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(\(R37165,)Tj
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(1:10,000\))Tj
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2.4264 0 Td
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(PBST,)Tj
2.6702 0 Td
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1.7292 0 Td
(widefield)Tj
3.8777 0 Td
(image)Tj
2.6306 0 Td
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2.4207 0 Td
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1.2756 0 Td
(EVOS)Tj
2.7099 0 Td
(FL)Tj
1.2813 0 Td
(Auto)Tj
2.2393 0 Td
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1.7972 0 Td
(Imaging)Tj
3.5603 0 Td
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-30.3078 -1.2983 Td
(\(Invitrogen\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 151.9937 cm
BT
/F0 1 Tf
1 TL
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(FITC-dextran)Tj
6.0614 0 Td
(permeability)Tj
5.5371 0 Td
(assay)Tj
ET
Q
q
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10 0 0 10 200.0125 134.9858 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
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1.7801 0 Td
(hCMVECS)Tj
4.7055 0 Td
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2.1259 0 Td
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2.9084 0 Td
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1.8368 0 Td
(collagen)Tj
3.515 0 Td
(I)Tj
0.5612 0 Td
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(0.4)Tj
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[()]TJ
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(pore)Tj
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(polycarbonate)Tj
5.8904 0 Td
(mem-)Tj
-35.0643 -1.2982 Td
(brane)Tj
2.5058 0 Td
(inserts)Tj
2.8459 0 Td
(\(cat#)Tj
2.194 0 Td
(3413,)Tj
2.3471 0 Td
(Costar\))Tj
3.1975 0 Td
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0.9524 0 Td
(the)Tj
1.4683 0 Td
(density)Tj
3.1011 0 Td
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1.0148 0 Td
(20,000)Tj
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1.9219 0 Td
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1.5307 0 Td
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(M199)Tj
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(growth)Tj
3.0727 0 Td
(media)Tj
-34.3726 -1.2983 Td
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2.1203 0 Td
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1.3833 0 Td
(48)Tj
1.1735 0 Td
(h.)Tj
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(70)Tj
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4.8472 0 Td
(isothiocyanate)Tj
5.9697 0 Td
(\(FITC\)dextran)Tj
6.6274 0 Td
(\(cat#)Tj
2.194 0 Td
(46945,)Tj
-32.876 -1.3039 Td
(Sigma)Tj
2.6815 0 Td
(Aldrich\))Tj
3.6057 0 Td
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3.0387 0 Td
(to)Tj
1.0261 0 Td
(10)Tj
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1.1735 0 Td
[()]TJ
/F7 1 Tf
(g/mL)Tj
2.8744 0 Td
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1.0374 0 Td
(M199)Tj
2.5399 0 Td
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3.0727 0 Td
(media)Tj
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(added)Tj
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